4KKA: H/Cl exchange transporter ClcA

Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide. Determined by X-ray diffraction at 3.0 Å resolution. Released 21 Aug 2013.

Method: X-ray diffraction
Resolution: 3.0 Å
Organisms: Escherichia coli, Mus musculus
Chains: 6
Atoms: 13,206
Mol. weight: 188.94 kDa
Released: 21 Aug 2013

Explore 4KKA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4KKA contains 76 α-helices and 95 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 26 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	18-26	9
α-helix	33-70	38
α-helix	75-100	26
α-helix	102-104	3
β-strand	106	1	1
α-helix	109-116	8
α-helix	124-138	15
β-strand	146	1	2
α-helix	148-165	18
α-helix	171-190	20
α-helix	193-199	7
α-helix	200-204	5
α-helix	215-233	19
α-helix	249-251	3
α-helix	253-284	32
α-helix	288-308	21
α-helix	310-312	3
α-helix	319-324	6
α-helix	330-348	19
β-strand	353	1	1
β-strand	355	1	2
α-helix	359-378	20
α-helix	380-382	3
α-helix	386-394	9
α-helix	396-397	2
α-helix	398-402	5
α-helix	405-416	12
α-helix	419-421	3
α-helix	422-438	17
α-helix	444-458	15

Chain B: 26 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	19-25	7
α-helix	33-70	38
α-helix	75-100	26
α-helix	102-104	3
β-strand	106	1	3
α-helix	109-116	8
α-helix	124-138	15
β-strand	146	1	4
α-helix	148-165	18
α-helix	171-190	20
α-helix	193-199	7
α-helix	200-204	5
α-helix	215-233	19
α-helix	249-251	3
α-helix	253-284	32
α-helix	288-308	21
α-helix	310-312	3
α-helix	319-324	6
α-helix	330-348	19
β-strand	353	1	3
β-strand	355	1	4
α-helix	359-378	20
α-helix	380-382	3
α-helix	386-394	9
α-helix	396-397	2
α-helix	398-402	5
α-helix	405-416	12
α-helix	419-421	3
α-helix	422-438	17
α-helix	444-454	11

Chain C: 7 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	5
β-strand	10-12	3	6
β-strand	16-25	10	5
β-strand	34-39	6	6
β-strand	45-51	7	6
β-strand	58-60	3	6
β-strand	68-73	6	5
β-strand	78-86	9	5
α-helix	88-90	3
β-strand	92-100	9	6
β-strand	107-111	5	6
β-strand	115-119	5	6
α-helix	122-124	3
β-strand	125	1	7
α-helix	126-127	2
β-strand	128-131	4	8
α-helix	133-135	3
β-strand	143-153	11	8
β-strand	154	1	7
β-strand	159-162	4	9
α-helix	163-165	3
β-strand	171-173	3	8
α-helix	174-176	3
β-strand	177-179	3	8
β-strand	182-192	11	8
β-strand	201-207	7	9
α-helix	208-210	3
β-strand	212-218	7	9

Chain D: 7 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	10
β-strand	10-13	4	11
β-strand	19-25	7	10
β-strand	33-37	5	11
β-strand	44-48	5	11
α-helix	49-51	3
β-strand	52-53	2	11
α-helix	54	1
β-strand	61-66	6	10
β-strand	69-74	6	10
α-helix	79-81	3
β-strand	83-89	7	11
β-strand	96-97	2	11
β-strand	102-105	4	11
β-strand	110	1	12
β-strand	113-117	5	13
α-helix	118-120	3
α-helix	121-124	4
β-strand	128-138	11	13
β-strand	139	1	12
β-strand	144-149	6	14
β-strand	152-154	3	14
β-strand	158-162	5	13
α-helix	163-166	4
β-strand	172-181	10	13
α-helix	182-186	5
β-strand	190-196	7	14
β-strand	204-209	6	14

Chain E: 5 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	15
β-strand	10-12	3	16
β-strand	17-25	9	15
β-strand	34-39	6	16
β-strand	45-51	7	16
β-strand	58-60	3	16
β-strand	68-73	6	15
β-strand	78-84	7	15
β-strand	92-101	10	16
β-strand	106-111	6	16
β-strand	115-119	5	16
β-strand	125	1	17
α-helix	126-127	2
β-strand	128-132	5	18
β-strand	143-153	11	18
β-strand	154	1	17
β-strand	159-162	4	19
α-helix	163-165	3
β-strand	167	1	19
β-strand	171-173	3	18
α-helix	174-176	3
β-strand	177-179	3	18
β-strand	182-192	11	18
α-helix	193-195	3
β-strand	202-207	6	19
α-helix	208-210	3
β-strand	212-217	6	19

Chain F: 5 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	20
β-strand	10-13	4	21
β-strand	15	1	22
β-strand	17	1	22
β-strand	18-25	8	20
β-strand	33-37	5	21
β-strand	44-48	5	21
β-strand	52-53	2	21
α-helix	54	1
β-strand	61-66	6	20
β-strand	69-75	7	20
α-helix	79-81	3
β-strand	84-89	6	21
β-strand	96-97	2	21
β-strand	101-105	5	21
β-strand	110	1	23
β-strand	114-117	4	24
α-helix	121-124	4
β-strand	128-138	11	24
β-strand	139	1	23
β-strand	144-149	6	25
β-strand	152-154	3	25
β-strand	158-162	5	24
α-helix	163-166	4
β-strand	172-181	10	24
α-helix	182-186	5
β-strand	190-197	8	25
β-strand	200-209	10	25

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
H(+)/Cl(-) exchange transporter ClcA	A, B	protein	446	Escherichia coli	P37019 (AlphaFold model)
Fab, heavy chain	C, E	protein	222	Mus musculus
Fab, light chain	D, F	protein	211	Mus musculus

Sequence of entity 1 (A, B), FASTA

>4KKA_1 H(+)/Cl(-) exchange transporter ClcA (chains A, B)
MRRRQLIRQLLERDKTPLAILFMAAVVGTLVGLAAVAFDKGVAWLQNQRMGALVHTADNY
PLLLTVAFLCSAVLAMFGYFLVRKYAPEAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGG
LGTLGGGMVLGRAGPTVQIGGNIGRMVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLA
GILFIIEEMRPQFRYTLISIKAVFIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLY
LILGIIFGIFGPIFNKWVLGMQDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGG
GFNLIPIATAGNFSMGMLVFIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVA
VELFPQYHLEAGTFAIAGMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLL
AQFTGGKPLYSAILARTLAKQEAEQK

Sequence of entity 2 (C, E), FASTA

>4KKA_2 Fab, heavy chain (chains C, E)
EVRLLESGGGLVQPGGSLKLSCAASGFDYSRYWMSWVRQAPGKGLKWIGEINPVSSTINY
TPSLKDKFIISRDNAKDTLYLQISKVRSEDTALYYCARLYYGYGYWYFDVWGAGTTVTVS
SAKTTPPSVYPLAPGSAAAAASMVTLGCLVKGYFPEPVTVTWNSGSLAAGVHTFPAVLQA
ALYTLSSSVTVPSSSWPSETVTCNVAHPASSTKVDKKIVPRA

Sequence of entity 3 (D, F), FASTA

>4KKA_3 Fab, light chain (chains D, F)
DIVLTQSPAIMSAAPGDKVTMTCSASSSVSYIHWYQQKSGTSPKRWIYDTSKLTSGVPVR
FSGSGSGTSYSLTINTMEAEDAATYYCQQWSSHPQTFGGGTKLEILRADAAPTVSIFPPS
SEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTL
TKDEYERHNSYTCEATHKTSTSPIVKSFNRA

Primary citation

Fluoride-dependent interruption of the transport cycle of a CLC Cl(-)/H(+) antiporter. Lim, H.H., Stockbridge, R.B., Miller, C. Nat Chem Biol (2013) 9:721-725. DOI 10.1038/nchembio.1336 · PubMed

Other PDB entries of the same protein (UniProt P37019 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4ENE 2.4 Å, Structure of the N- and C-terminal trimmed ClC-ec1 Cl-/H+ antiporter and Fab Complex
1OTS 2.51 Å, Structure of the Escherichia coli ClC Chloride channel and Fab Complex
8GA1 2.6 Å, CLC-ec1 R230C/L249C/C85A at pH 4.5 100mM Cl Swap
8GA5 2.6 Å, CLC-ec1 L25C/A450C/C85A at pH 4.5 100mM Cl Intermediate
6V2J 2.62 Å, Crystal structure of ClC-ec1 triple mutant (E113Q, E148Q, E203Q)
6ADB 2.69 Å, Crystal structure of the E148N mutant CLC-ec1 in 20mM bromide
3DET 2.8 Å, Structure of the E148A, Y445A doubly ungated mutant of E.coli CLC_Ec1, Cl-/H+ antiporter
4KKL 2.85 Å, Structure of the E148A mutant of CLC-ec1 delta NC construct in 100mM fluoride
4KK8 2.86 Å, Structure of the E148Q mutant of CLC-ec1 deltaNC construct in 100mM fluoride
4KJQ 2.88 Å, Structure of the CLC-ec1 deltaNC construct in 100mM fluoride
3EJZ 2.9 Å, Structure of E203V mutant E.coli Cl-/H+ exchanger, CLC-ec1
7CVT 2.9 Å, Crystal structure of the C85A/L194A/H234C mutant CLC-ec1 with Fab fragment

Browse structure collections

About this viewer

MolViewer shows 4KKA directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.