Crystal Structure of SARS-CoV papain-like protease C112S mutant in complex with ubiquitin. Determined by X-ray diffraction at 1.4 Å resolution. Released 12 Feb 2014.
Explore 4M0W in 3D Show helices and sheets RCSB PDB PDBe
4M0W contains 14 α-helices and 30 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 5-11 | 7 | 1 |
| β-strand | 18-23 | 6 | 1 |
| α-helix | 28-31 | 4 | |
| β-strand | 35-37 | 3 | 1 |
| β-strand | 40-41 | 2 | 1 |
| α-helix | 49-51 | 3 | |
| β-strand | 55-58 | 4 | 1 |
| α-helix | 63-73 | 11 | |
| α-helix | 80-91 | 12 | |
| β-strand | 98-99 | 2 | 2 |
| β-strand | 102-103 | 2 | 2 |
| α-helix | 112-121 | 10 | |
| β-strand | 128 | 1 | 3 |
| α-helix | 131-141 | 11 | |
| α-helix | 146-156 | 11 | |
| β-strand | 164 | 1 | 4 |
| α-helix | 166-174 | 9 | |
| β-strand | 177 | 1 | 3 |
| β-strand | 183-190 | 8 | 5 |
| β-strand | 194-201 | 8 | 5 |
| α-helix | 202-205 | 4 | |
| β-strand | 207-209 | 3 | 4 |
| α-helix | 214-219 | 6 | |
| β-strand | 221-224 | 4 | 5 |
| β-strand | 230-239 | 10 | 5 |
| β-strand | 242-255 | 14 | 4 |
| β-strand | 261-267 | 7 | 4 |
| β-strand | 272-279 | 8 | 4 |
| β-strand | 283-287 | 5 | 4 |
| β-strand | 290-294 | 5 | 4 |
| β-strand | 296-307 | 12 | 4 |
| β-strand | 310-312 | 3 | 5 |
| α-helix | 315-318 | 4 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2-6 | 5 | 6 |
| β-strand | 12-16 | 5 | 6 |
| β-strand | 22 | 1 | 7 |
| α-helix | 23-34 | 12 | |
| β-strand | 41-45 | 5 | 6 |
| β-strand | 48-49 | 2 | 6 |
| α-helix | 50 | 1 | |
| β-strand | 55 | 1 | 7 |
| α-helix | 57-59 | 3 | |
| β-strand | 66-71 | 6 | 6 |
| β-strand | 75 | 1 | 4 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Replicase polyprotein 1a | A | protein | 327 | SARS coronavirus | P0C6U8 |
| Ubiquitin | B | protein | 76 | Bos taurus | P62992 (AlphaFold model) |
>4M0W_1 Replicase polyprotein 1a (chains A) MEVKTIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLP SDDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNSYLSSVLLA LQQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLE SAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQES SFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGP VTDVFYKETSYTTTIKPVSLEHHHHHH
>4M0W_2 Ubiquitin (chains B) MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG
Water and common crystallization additives (GOL, NA) are not listed.
Structural basis for catalysis and ubiquitin recognition by the severe acute respiratory syndrome coronavirus papain-like protease. Chou, C.-Y., Lai, H.-Y., Chen, H.-Y. et al. Acta Crystallogr D Biol Crystallogr (2014) 70:572-581. DOI 10.1107/S1399004713031040 · PubMed
Other PDB entries of the same protein (UniProt P0C6U8), best resolution first:
MolViewer shows 4M0W directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.