4MQW: Follicle-stimulating hormone

Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor (P31). Determined by X-ray diffraction at 2.9 Å resolution. Released 9 Apr 2014.

Method: X-ray diffraction
Resolution: 2.9 Å
Organism: Homo sapiens
Chains: 9
Atoms: 11,798
Mol. weight: 199.82 kDa
Ligands: JEF, NAG
Released: 9 Apr 2014

Explore 4MQW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4MQW contains 18 α-helices and 122 β-strands across 9 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	9	1	1
β-strand	11-14	4	1
β-strand	20	1	2
β-strand	23	1	2
β-strand	26-38	13	1
α-helix	39-40	2
α-helix	41-44	4
β-strand	53-56	4	1
β-strand	59-70	12	3
β-strand	74-85	12	3
β-strand	91	1	4

Chain B: 1 helix, 6 β-strands

Element	Residues	Length	Sheet
β-strand	4-14	11	1
α-helix	15-17	3
β-strand	19-35	17	1
β-strand	50-63	14	5
β-strand	72-85	14	5
β-strand	87	1	4
β-strand	92-95	4	1

Chain D: 2 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	9	1	12
β-strand	11-14	4	12
β-strand	20	1	13
β-strand	23	1	13
β-strand	26-38	13	12
α-helix	39-40	2
α-helix	41-44	4
β-strand	53-57	5	12
β-strand	58-70	13	14
β-strand	74-86	13	14
β-strand	91	1	15

Chain E: 2 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	3-14	12	12
α-helix	15-17	3
β-strand	19-35	17	12
β-strand	50-63	14	16
α-helix	64	1
β-strand	66	1	17
β-strand	69	1	17
β-strand	72-85	14	16
β-strand	87	1	15
β-strand	92-95	4	12

Chain G: 3 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	9	1	24
β-strand	11-14	4	24
β-strand	20	1	25
β-strand	23	1	25
α-helix	24-25	2
β-strand	26-38	13	24
α-helix	39-40	2
α-helix	41-44	4
β-strand	53-57	5	24
β-strand	59-70	12	26
β-strand	74-85	12	26
β-strand	91	1	27

Chain H: 1 helix, 9 β-strands

Element	Residues	Length	Sheet
β-strand	3-14	12	24
α-helix	15-17	3
β-strand	19-35	17	24
β-strand	39	1	26
β-strand	50-63	14	28
β-strand	66	1	29
β-strand	69	1	29
β-strand	72-85	14	28
β-strand	87	1	27
β-strand	92-95	4	24

Chain X: 1 helix, 24 β-strands

Element	Residues	Length	Sheet
β-strand	24-25	2	6
β-strand	29-33	5	6
β-strand	50-54	5	6
β-strand	60-61	2	7
β-strand	74-78	5	6
β-strand	85-86	2	7
β-strand	91	1	8
β-strand	99-105	7	6
β-strand	110-111	2	7
β-strand	116	1	8
β-strand	124-130	7	6
β-strand	143	1	9
β-strand	147-152	6	6
β-strand	159-160	2	10
β-strand	169	1	9
β-strand	173-176	4	6
β-strand	184-185	2	10
β-strand	193-194	2	11
β-strand	195-199	5	6
β-strand	217-218	2	11
β-strand	222-224	3	6
β-strand	243-245	3	6
β-strand	266-268	3	6
α-helix	272-278	7
β-strand	345-347	3	6

Chain Y: 3 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	24-25	2	18
β-strand	29-33	5	18
β-strand	50-54	5	18
β-strand	60-61	2	19
β-strand	74-78	5	18
β-strand	85-86	2	19
β-strand	91	1	20
β-strand	99-105	7	18
β-strand	110-111	2	19
β-strand	116	1	20
β-strand	124-130	7	18
β-strand	143	1	21
β-strand	147-152	6	18
β-strand	159-160	2	22
β-strand	169	1	21
β-strand	173-176	4	18
β-strand	184-185	2	22
β-strand	193-194	2	23
β-strand	195-199	5	18
α-helix	211-213	3
β-strand	217-218	2	23
β-strand	222-224	3	18
α-helix	238-240	3
β-strand	243-245	3	18
β-strand	266-268	3	18
α-helix	273-279	7
β-strand	345-347	3	18

1 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Glycoprotein hormones, alpha polypeptide	A, D, G	protein	102	Homo sapiens	P01215 (AlphaFold model)
Follitropin subunit beta	B, E, H	protein	111	Homo sapiens	P01225 (AlphaFold model)
Follicle-stimulating hormone receptor	X, Y, Z	protein	361	Homo sapiens	P23945 (AlphaFold model)

Sequence of entity 1 (A, D, G), FASTA

>4MQW_1 Glycoprotein hormones, alpha polypeptide (chains A, D, G)
APDVQDCPECTLQENPLFSQPGAPILQCMGCCFSRAYPTPLRSKKTMLVQKNVTSESTCC
VAKSYNRVTVMGGFKVENHTACHCSTCYYHKSAAAHHHHHHH

Sequence of entity 2 (B, E, H), FASTA

>4MQW_2 Follitropin subunit beta (chains B, E, H)
NSCELTNITIAIEKEECRFCISINTTWCAGYCYTRDLVYKDPARPKIQKTCTFKELVYET
VRVPGCAHHADSLYTYPVATQCHCGKCDSDSTDCTVRGLGPSYCSFGEMKE

Sequence of entity 3 (X, Y, Z), FASTA

>4MQW_3 Follicle-stimulating hormone receptor (chains X, Y, Z)
SGCHHRICHCSNRVFLCQESKVTEIPSDLPRNAIELRFVLTKLRVIQKGAFSGFGDLEKI
EISQNDVLEVIEADVFSNLPKLHEIRIEKANNLLYINPEAFQNLPNLQYLLISNTGIKHL
PDVHKIHSLQKVLLDIQDNINIHTIERNSFVGLSFESVILWLNKNGIQEIHNSAFNGTQL
DELNLSDNNNLEELPNDVFHGASGPVILDISRTRIHSLPSYGLENLKKLRARSTYNLKKL
PTLEKLVALMEASLTYPSHCCAFANWRRQISELHPICNKSILRQEVDYMTQARGQRSSLA
EDNESSYSRGFDMTYTEFDYDLCNEVVDVTCSPKPDAFNPCEDIMGYNILRAAAHHHHHH
H

Ligands and cofactors

ID	Name	Formula	Copies
JEF	O-(O-(2-aminopropyl)-O'-(2-methoxyethyl)polypropylene glycol 500)	C30 H63 N O10	1
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	15

Water and common crystallization additives (EDO) are not listed.

Primary citation

Evidence for Follicle-stimulating Hormone Receptor as a Functional Trimer. Jiang, X., Fischer, D., Chen, X. et al. J Biol Chem (2014) 289:14273-14282. DOI 10.1074/jbc.M114.549592 · PubMed

Other PDB entries of the same protein (UniProt P01215 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9YXD 2.29 Å, Crystal structure of recombinant human follicle stimulating hormone in complex with an…
7UTZ 2.4 Å, Human thyrotropin analog TR1402 bound to human Thyrotropin receptor in complex with…
4AY9 2.5 Å, Structure of follicle-stimulating hormone in complex with the entire ectodomain of its…
1HCN 2.6 Å, Structure of human chorionic gonadotropin at 2.6 Å resolution from mad analysis of the…
8I2G 2.8 Å, FSHR-Follicle stimulating hormone-compound 716340-Gs complex
7T9I 2.9 Å, Native human TSH bound to human Thyrotropin receptor in complex with miniGs399…
1XWD 2.92 Å, Crystal Structure of Human Follicle Stimulating Hormone Complexed with its Receptor
7XW5 2.96 Å, TSHR-thyroid stimulating hormone-Gs-ML109 complex
1FL7 3.0 Å, Human follicle stimulating hormone
1HRP 3.0 Å, Crystal structure of human chorionic gonadotropin
7FIH 3.2 Å, luteinizing hormone/choriogonadotropin receptor(S277I)-chorionic…
1QFW 3.5 Å, Ternary complex of human chorionic gonadotropin with fv anti alpha subunit and fv anti…

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