8I2G: PDB entry 8I2G

Name: PDB 8I2G: FSHR-Follicle stimulating hormone-compound 716340-Gs complex
Published: 2023-03-29
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: hormone

FSHR-Follicle stimulating hormone-compound 716340-Gs complex. Determined by electron microscopy at 2.8 Å resolution. Released 29 Mar 2023.

Method: Electron microscopy
Resolution: 2.8 Å
Organisms: Cricetulus griseus, Homo sapiens, Lama glama
Chains: 7
Atoms: 12,694
Mol. weight: 196.97 kDa
Ligands: CLR, PLM, MYR, NAG
Released: 29 Mar 2023

Explore 8I2G in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8I2G contains 45 α-helices and 83 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	13-38	26
β-strand	40-47	8	1
α-helix	53-58	6
β-strand	207-213	7	1
β-strand	218-224	7	1
α-helix	234-238	5
β-strand	243-248	6	1
α-helix	265-278	14
β-strand	286-291	6	1
α-helix	294-302	9
α-helix	308-311	4
α-helix	313-315	3
α-helix	326-327	2
α-helix	332-351	20
β-strand	359-363	5	1
α-helix	370-390	21

Chain B: 5 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
α-helix	95	1
β-strand	102-105	4	4
β-strand	111-115	5	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	175-181	7	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	217-222	6	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
α-helix	272	1
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	293-298	6	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain G: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	27-29	3
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain N: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	10-11	2	10
β-strand	17-25	9	9
α-helix	29-31	3
β-strand	34-39	6	10
β-strand	45-51	7	10
β-strand	58-60	3	10
β-strand	69-73	5	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-98	7	10
α-helix	108-111	4
β-strand	118	1	10
β-strand	122-125	4	10

Chain R: 18 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	24-26	3	11
β-strand	29-31	3	11
β-strand	50-54	5	11
β-strand	60-61	2	12
β-strand	74-78	5	11
β-strand	85-86	2	12
β-strand	99-103	5	11
β-strand	105	1	13
β-strand	110-111	2	12
α-helix	113-115	3
β-strand	124-126	3	11
β-strand	130	1	13
β-strand	143	1	14
β-strand	147-150	4	11
β-strand	159-160	2	15
β-strand	169	1	14
β-strand	173-176	4	11
β-strand	184-185	2	15
β-strand	194	1	16
β-strand	195-199	5	11
β-strand	218	1	16
β-strand	222-224	3	11
β-strand	243-245	3	11
β-strand	266-268	3	11
α-helix	272-278	7
α-helix	290-293	4
α-helix	333-335	3
β-strand	345-347	3	11
α-helix	349-351	3
α-helix	363-389	27
α-helix	393-394	2
α-helix	396-424	29
α-helix	429-438	10
α-helix	440-472	33
α-helix	477-479	3
α-helix	483-502	20
α-helix	525-556	32
α-helix	564-596	33
α-helix	604-614	11
α-helix	616-622	7
α-helix	623-627	5
α-helix	631-643	13

Chain X: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	7-8	2
β-strand	9	1	17
β-strand	11-13	3	17
β-strand	27-38	12	17
α-helix	39-40	2
α-helix	41-44	4
β-strand	53-56	4	17
β-strand	59-70	12	18
β-strand	74-85	12	18
β-strand	91	1	19

Chain Y: 1 helix, 7 β-strands

Element	Residues	Length	Sheet
β-strand	2-14	13	17
β-strand	19-35	17	17
α-helix	45-46	2
β-strand	47	1	17
β-strand	50-63	14	20
β-strand	72-85	14	20
β-strand	87	1	19
β-strand	92-95	4	17

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
chimera of Guanine nucleotide-binding protein G(s) subunit alpha and Guanine nucleotide-binding…	A	protein	259	Cricetulus griseus, Homo sapiens	Q5JWF2 (AlphaFold model), Q8R4A8 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nb35	N	protein	128	Lama glama
Follicle-stimulating hormone receptor	R	protein	678	Homo sapiens	P23945
Glycoprotein hormones alpha chain	X	protein	92	Homo sapiens	P01215
Follitropin subunit beta	Y	protein	109	Homo sapiens	P01225

Sequence of entity 1 (A), FASTA

>8I2G_1 chimera of Guanine nucleotide-binding protein G(s) subunit alpha and Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas (chains A)
MNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILH
GGSGGSGGTTGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNM
VIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEF
ARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRV
FNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>8I2G_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>8I2G_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>8I2G_4 Nb35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSS

Sequence of entity 5 (R), FASTA

>8I2G_5 Follicle-stimulating hormone receptor (chains R)
CHHRICHCSNRVFLCQESKVTEIPSDLPRNAIELRFVLTKLRVIQKGAFSGFGDLEKIEI
SQNDVLEVIEADVFSNLPKLHEIRIEKANNLLYINPEAFQNLPNLQYLLISNTGIKHLPD
VHKIHSLQKVLLDIQDNINIHTIERNSFVGLSFESVILWLNKNGIQEIHNCAFNGTQLDE
LNLSDNNNLEELPNDVFHGASGPVILDISRTRIHSLPSYGLENLKKLRARSTYNLKKLPT
LEKLVALMEASLTYPIHCCAFANWRRQISELHPICNKSILRQEVDYMTQARGQRSSLAED
NESSYSRGFDMTYTEFDYDLCNEVVDVTCSPKPDAFNPCEDIMGYNILRVLIWFISILAI
TGNIIVLVILTTSQYKLTVPRFLMCNLAFADLCIGIYLLLIASVDIHTKSQYHNYAIDWQ
TGAGCDAAGFFTVFASELSVYTLTAITLERWHTITHAMQLDCKVQLRHAASVMVMGWIFA
FAAALFPIFGISSYMKVSICLPMDIDSPLSQLYVMSLLVLNVLAFVVICGCYIHIYLTVR
NPNIVSSSSDTRIAKRMAMLIFTDFLCMAPISFFAISASLKVPLITVSKAKILLVLFHPI
NSCANPFLYAIFTKNFRRDFFILLSKCGCYEMQAQIYRTETSSTVHNTHPRNGHCSSAPR
VTNGSTYILVPLSHLAQN

Sequence of entity 6 (X), FASTA

>8I2G_6 Glycoprotein hormones alpha chain (chains X)
APDVQDCPECTLQENPLFSQPGAPILQCMGCCFSRAYPTPLRSKKTMLVQKNVTSESTCC
VAKSYNRVTVMGGFKVENHTACHCSTCYYHKS

Sequence of entity 7 (Y), FASTA

>8I2G_7 Follitropin subunit beta (chains Y)
NSCELTNITIAIEKEECRFCISINTTWCAGYCYTRDLVYKDPARPKIQKTCTFKELVYET
VRVPGCAHHADSLYTYPVATQCHCGKCDSDSTDCTVRGLGPSYCSFGEM

Ligands and cofactors

ID	Name	Formula	Copies
CLR	Cholesterol	C27 H46 O	13
PLM	Palmitic acid	C16 H32 O2	3
MYR	Myristic acid	C14 H28 O2	2
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	4
O6F	N-tert-butyl-N-ethyl-8-methoxy-9-propan-2-yloxy-1-thiophen-2-yl-5,6-dihydroimid…	C26 H33 N3 O3 S	1

Primary citation

Mechanism of hormone and allosteric agonist mediated activation of follicle stimulating hormone receptor. Duan, J., Xu, P., Zhang, H. et al. Nat Commun (2023) 14:519-519. DOI 10.1038/s41467-023-36170-3 · PubMed

Other PDB entries of the same protein (UniProt Q5JWF2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9BKK 2.51 Å, Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
8WW2 2.79 Å, GPR3/Gs complex
8JLQ 2.84 Å, Fenoldopam-bound hTAAR1-Gs protein complex
9DQJ 2.9 Å, CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-3945
9YGZ 3.04 Å, Cryo-EM structure of active mutant human green cone opsin (E129Q) in complex with…
9WNF 3.13 Å, 5HT2AR-miniGq heterotrimer in complex with a selective agonist IHCH-2330
8ZH8 3.19 Å, Human GPR103 -Gq complex bound to QRFP26
8JHI 3.2 Å, FZD3-Gs complex
9WND 3.2 Å, 5HT2AR-miniGq heterotrimer in complex with a psychedelic psilocin
8JLP 3.23 Å, Ralmitaront(RO-6889450)-bound hTAAR1-Gs protein complex
8GY7 3.3 Å, Cryo-EM structure of ACTH-bound melanocortin-2 receptor in complex with MRAP1 and Gs…
8JHB 3.3 Å, FZD6 Gs complex

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