Elongation factor Tu:Ts complex in a near GTP conformation. Determined by X-ray diffraction at 3.6 Å resolution. Released 6 May 2015.
Explore 4PC7 in 3D Show helices and sheets RCSB PDB PDBe
4PC7 contains 20 α-helices and 43 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 11-18 | 8 | 1 |
| α-helix | 24-36 | 13 | |
| β-strand | 67-70 | 4 | 1 |
| β-strand | 75-78 | 4 | 1 |
| α-helix | 89-97 | 9 | |
| β-strand | 100-106 | 7 | 1 |
| α-helix | 113-124 | 12 | |
| β-strand | 129-135 | 7 | 1 |
| α-helix | 137-139 | 3 | |
| α-helix | 143-159 | 17 | |
| α-helix | 164-166 | 3 | |
| β-strand | 169-170 | 2 | 1 |
| α-helix | 175-178 | 4 | |
| α-helix | 182-197 | 16 | |
| β-strand | 211-213 | 3 | 2 |
| β-strand | 217-220 | 4 | 3 |
| β-strand | 224-230 | 7 | 3 |
| β-strand | 233 | 1 | 2 |
| β-strand | 241 | 1 | 4 |
| β-strand | 244 | 1 | 5 |
| β-strand | 245 | 1 | 2 |
| β-strand | 251 | 1 | 5 |
| β-strand | 254 | 1 | 4 |
| β-strand | 255-260 | 6 | 3 |
| β-strand | 263-265 | 3 | 3 |
| β-strand | 273-278 | 6 | 3 |
| β-strand | 291-293 | 3 | 2 |
| β-strand | 299-306 | 8 | 6 |
| β-strand | 322 | 1 | 7 |
| β-strand | 329-332 | 4 | 8 |
| β-strand | 335-337 | 3 | 8 |
| β-strand | 339 | 1 | 9 |
| β-strand | 342 | 1 | 6 |
| β-strand | 345 | 1 | 10 |
| β-strand | 347 | 1 | 10 |
| β-strand | 350 | 1 | 7 |
| β-strand | 358-361 | 4 | 6 |
| β-strand | 362 | 1 | 9 |
| β-strand | 363-368 | 6 | 6 |
| β-strand | 373 | 1 | 11 |
| β-strand | 375-377 | 3 | 8 |
| β-strand | 378 | 1 | 12 |
| β-strand | 381 | 1 | 12 |
| β-strand | 387 | 1 | 11 |
| β-strand | 388-391 | 4 | 6 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 5-13 | 9 | |
| α-helix | 19-28 | 10 | |
| α-helix | 33-42 | 10 | |
| β-strand | 59-64 | 6 | 13 |
| β-strand | 69-76 | 8 | 13 |
| α-helix | 82-84 | 3 | |
| α-helix | 88-102 | 15 | |
| α-helix | 107-113 | 7 | |
| α-helix | 115-125 | 11 | |
| β-strand | 130-138 | 9 | 13 |
| β-strand | 141-147 | 7 | 14 |
| β-strand | 151-157 | 7 | 14 |
| α-helix | 164-174 | 11 | |
| α-helix | 182-184 | 3 | |
| α-helix | 187-198 | 12 | |
| α-helix | 212-226 | 15 | |
| α-helix | 232-233 | 2 | |
| β-strand | 252-259 | 8 | 14 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Elongation factor Tu 1 | A | protein | 394 | Escherichia coli | P0CE47 (AlphaFold model) |
| Elongation factor Ts | C | protein | 282 | Escherichia coli | P0A6P1 (AlphaFold model) |
>4PC7_1 Elongation factor Tu 1 (chains A) MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARG ITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHI LLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALE GDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIK PHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMV VTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG
>4PC7_2 Elongation factor Ts (chains C) AEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKSGAIKAAKKAGNVAADGV IKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVAGKITDVEVLKAQFEEERVA LVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKGADEELVKHIAMHVAASKPEFI KPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEGRMKKFTGEVSLTGQPFVMEPSKT VGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEVAAMSKQS
| ID | Name | Formula | Copies |
|---|---|---|---|
| MG | Magnesium ion | Mg | 1 |
| PUL | (1S,2S,3E,5E,7E,10S,11S,12S)-12-[(2R,4E,6E,8Z,10R,12E,14E,16Z,18S,19Z)-10,18-di… | C47 H66 O13 | 1 |
| GNP | Phosphoaminophosphonic acid-guanylate ester | C10 H17 N6 O13 P3 | 1 |
Structural outline of the detailed mechanism for elongation factor Ts-mediated guanine nucleotide exchange on elongation factor Tu. Thirup, S.S., Van, L.B., Nielsen, T.K. et al. J Struct Biol (2015) 191:10-21. DOI 10.1016/j.jsb.2015.06.011 · PubMed
Other PDB entries of the same protein (UniProt P0CE47 (AlphaFold model), which also has an AlphaFold model), best resolution first:
MolViewer shows 4PC7 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.