4PL7: Actin,Thymosin beta-4

Structure of Komagataella pastoris actin-thymosin beta4 hybrid. Determined by X-ray diffraction at 2.3 Å resolution. Released 22 Oct 2014.

Method
X-ray diffraction
Resolution
2.3 Å
Organisms
Komagataella pastoris, Homo sapiens
Chains
2
Atoms
6,267
Mol. weight
98.06 kDa
Ligands
ATP, CA
Released
22 Oct 2014

Explore 4PL7 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4PL7 contains 57 α-helices and 44 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 28 helices, 22 β-strands

ElementResiduesLengthSheet
β-strand8-1251
β-strand16-2161
β-strand2412
β-strand29-3241
β-strand3513
β-strand5413
α-helix56-594
β-strand6813
β-strand71-7224
β-strand75-7624
α-helix79-879
α-helix88-947
α-helix98-1003
β-strand103-10751
α-helix113-1219
α-helix122-1265
β-strand131-13661
α-helix137-1448
β-strand150-15565
β-strand160-16675
β-strand169-17025
α-helix172-1743
β-strand176-17835
α-helix182-19514
α-helix203-21614
α-helix223-2308
β-strand238-24146
β-strand247-25046
α-helix253-2564
α-helix258-2614
α-helix264-2674
α-helix274-2829
α-helix287-2948
β-strand297-30045
α-helix302-3054
α-helix309-32012
β-strand329-33025
α-helix335-3373
α-helix338-34710
α-helix350-3556
α-helix3561
β-strand357-35821
α-helix359-3646
α-helix395-4006
α-helix403-4053
β-strand40712
α-helix4081
β-strand412-41321
α-helix416-4194
α-helix420-4278
Chain B: 29 helices, 22 β-strands
ElementResiduesLengthSheet
β-strand8-1257
β-strand16-2167
β-strand2418
β-strand29-3247
β-strand3519
β-strand5419
α-helix56-594
β-strand6819
β-strand71-72210
β-strand75-76210
α-helix79-879
α-helix88-947
α-helix98-1003
β-strand103-10757
α-helix113-1219
α-helix122-1265
β-strand131-13667
α-helix137-1448
β-strand150-155611
β-strand160-166711
β-strand169-170211
α-helix172-1743
β-strand176-178311
α-helix182-19413
α-helix203-21614
α-helix223-2308
β-strand238-241412
β-strand247-250412
α-helix253-2564
α-helix258-2614
α-helix264-2674
α-helix274-2829
α-helix287-2948
β-strand297-300411
α-helix302-3054
α-helix309-32012
α-helix326-3272
β-strand329-330211
α-helix335-3373
α-helix338-34710
α-helix353-3553
α-helix3561
β-strand357-35827
α-helix359-3635
α-helix395-4006
α-helix403-4053
β-strand40718
α-helix4081
β-strand412-41327
α-helix416-4194
α-helix420-4278

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Actin,Thymosin beta-4A, Bprotein440Komagataella pastoris, Homo sapiensP62328 (AlphaFold model), Q9P4D1 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>4PL7_1 Actin,Thymosin beta-4 (chains A, B)
DGEDVAALVIDNGSGMCKAGYAGDDAPHTVFPSVVGRPRHQGVMVGMGQKDSFVGDEAQS
KRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRLAPEEHPVLLTEAPMNPKSNREKMT
QIMFETFNVPAFYVSIQAVLSLYASGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDL
AGRDLTDYLMKILSERGYTFSTSAEREIVRDIKEKLCYVALDFDQELQTSSQSSSIEKSY
ELPDGQVITIGNERFRAPEALFHPSVLGLEASGIDQTTYNSIMKCDVDVRKELYSNIVMS
GGTTMFPGIAERMQKELTALAPSSMKVKISAPPERKYSVWIGGSILASLGTFQQMWISKQ
EYDESGPSIVHLKCFASRGGSGGSSGGSASDKPDMAEIEKFDKSKLKKTETQEKNPLPSK
ETIEQEKQAGESGTLEVLFQ

Ligands and cofactors

IDNameFormulaCopies
ATPAdenosine-5'-triphosphateC10 H16 N5 O13 P32
CACalcium ionCa2

Primary citation

Structural basis of thymosin-beta 4/profilin exchange leading to actin filament polymerization. Xue, B., Leyrat, C., Grimes, J.M. et al. Proc Natl Acad Sci U S A (2014) 111:E4596-E4605. DOI 10.1073/pnas.1412271111 · PubMed

Other PDB entries of the same protein (UniProt P62328 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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