3TU5: Actin, alpha skeletal muscle

Actin complex with Gelsolin Segment 1 fused to Cobl segment. Determined by X-ray diffraction at 3.0 Å resolution. Released 26 Sept 2012.

Method: X-ray diffraction
Resolution: 3.0 Å
Organisms: Oryctolagus cuniculus, Homo sapiens, Mus musculus
Chains: 2
Atoms: 4,057
Mol. weight: 75.41 kDa
Ligands: ATP, CA
Released: 26 Sept 2012

Explore 3TU5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3TU5 contains 32 α-helices and 28 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	8-12	5	1
β-strand	16-21	6	1
β-strand	24	1	2
β-strand	29-32	4	1
β-strand	35-38	4	3
β-strand	53-54	2	3
α-helix	56-60	5
α-helix	62-64	3
β-strand	65-68	4	3
β-strand	71-72	2	4
β-strand	75-76	2	4
α-helix	79-87	9
α-helix	88-94	7
α-helix	98-100	3
β-strand	103-107	5	1
α-helix	113-125	13
β-strand	131-136	6	1
α-helix	137-144	8
β-strand	150-155	6	5
β-strand	160-166	7	5
β-strand	169-170	2	5
α-helix	172-174	3
β-strand	176-178	3	5
α-helix	182-195	14
α-helix	203-216	14
α-helix	223-231	9
β-strand	238-241	4	6
β-strand	247-250	4	6
α-helix	253-259	7
α-helix	264-267	4
α-helix	274-283	10
α-helix	287-289	3
α-helix	290-294	5
β-strand	297-300	4	5
α-helix	302-304	3
α-helix	309-320	12
β-strand	329-330	2	5
α-helix	338-346	9
α-helix	350-355	6
β-strand	357-358	2	1
α-helix	359-365	7
α-helix	367-372	6

Chain B: 10 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	6-10	5
β-strand	16-23	8	7
β-strand	26-29	4	7
α-helix	30-31	2
α-helix	32-34	3
β-strand	37-39	3	8
β-strand	43-51	9	7
β-strand	57-65	9	7
α-helix	71-87	17
α-helix	92	1
β-strand	93-98	6	7
α-helix	104-107	4
β-strand	115-117	3	8
α-helix	121-123	3
α-helix	125	1
β-strand	126	1	2
α-helix	127	1
α-helix	129-131	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin, alpha skeletal muscle	A	protein	377	Oryctolagus cuniculus	P68135 (AlphaFold model)
Gelsolin,Protein cordon-bleu,Thymosin beta-4	B	protein	297	Homo sapiens, Mus musculus	P06396 (AlphaFold model), P62328 (AlphaFold model), Q5NBX1 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>3TU5_1 Actin, alpha skeletal muscle (chains A)
MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
QSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREK
MTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRL
DLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK
SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNV
MSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIT
KQEYDEAGPSIVHRKCF

Sequence of entity 2 (B), FASTA

>3TU5_2 Gelsolin,Protein cordon-bleu,Thymosin beta-4 (chains B)
MNHKVHHHHHHIEGRHMGSVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGD
AYVILKTVQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFE
SATFLGYFKSGLKYKKGGVASKLRKVAEQTSEGRPKKPSYVEAESERSALLAAIRGHSGT
LSLRKVSSLASEELQSFRNAALGAPGLDKPQQEDLGLPPPPALPPPPAPAPQAPSASVTV
SRFSTGTPSNSVNARQALMDAIRSGTGAARLRKTETQEKNPLPSKETIEQEKQAGES

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	1
CA	Calcium ion	Ca	2

Water and common crystallization additives (MPD) are not listed.

Primary citation

Structural States and dynamics of the d-loop in actin. Durer, Z.A., Kudryashov, D.S., Sawaya, M.R. et al. Biophys J (2012) 103:930-939. DOI 10.1016/j.bpj.2012.07.030 · PubMed

Other PDB entries of the same protein (UniProt P68135 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4B1Y 1.29 Å, Structure of the Phactr1 RPEL-3 bound to G-actin
2FXU 1.35 Å, X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution.
4K41 1.4 Å, Crystal structure of actin in complex with marine macrolide kabiramide C
1QZ5 1.45 Å, Structure of rabbit actin in complex with kabiramide C
1WUA 1.45 Å, The structure of Aplyronine A-actin complex
2Q0U 1.45 Å, Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin
2V52 1.45 Å, Structure of MAL-RPEL2 complexed to G-actin
3MN5 1.5 Å, Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
2PBD 1.5 Å, Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*
5ZZA 1.53 Å, OdinProfilin/Rabbit Actin Complex
1J6Z 1.54 Å, Uncomplexed actin
1QZ6 1.6 Å, Structure of rabbit actin in complex with jaspisamide A

Browse structure collections

About this viewer

MolViewer shows 3TU5 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.