4UE5: SRP14

Name: PDB 4UE5: Structural basis for targeting and elongation arrest of Bacillus signal recognition particle
Published: 2015-09-09
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: translation

Structural basis for targeting and elongation arrest of Bacillus signal recognition particle. Determined by electron microscopy at 9.0 Å resolution. Released 9 Sept 2015.

Method: Electron microscopy
Resolution: 9.0 Å
Organism: CANIS LUPUS FAMILIARIS
Chains: 7
Atoms: 9,948
Mol. weight: 199.91 kDa
Released: 9 Sept 2015

Explore 4UE5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4UE5 contains 42 α-helices and 26 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 2 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	6-19	14
β-strand	21-33	13	1
β-strand	55-61	7	1
β-strand	66-72	7	1
α-helix	76-90	15

Chain C: 7 helices, 0 β-strands

Element	Residues	Length
α-helix	54-64	11
α-helix	72-90	19
α-helix	114-136	23
α-helix	141-164	24
α-helix	172-192	21
α-helix	196-215	20
α-helix	219-240	22

Chain D: 24 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	3-18	16
α-helix	25-41	17
α-helix	46-57	12
α-helix	58-62	5
α-helix	71-86	16
α-helix	93-96	4
β-strand	103-108	6	2
β-strand	109	1	3
β-strand	110	1	4
β-strand	112	1	4
α-helix	114-128	15
β-strand	132-136	5	2
α-helix	142-154	13
β-strand	159-160	2	2
α-helix	168-181	14
β-strand	186-190	5	2
β-strand	194	1	3
α-helix	201-209	9
β-strand	216-222	7	2
α-helix	223-225	3
α-helix	228-239	12
β-strand	243-248	6	2
α-helix	256-266	11
β-strand	270-274	5	2
β-strand	282-283	2	2
α-helix	287-295	9
α-helix	301-307	7
α-helix	317-324	8
α-helix	329-338	10
α-helix	346-349	4
α-helix	365-368	4
α-helix	376-381	6
α-helix	384-388	5
α-helix	392-398	7
α-helix	401-410	10
α-helix	414-432	19

Chain E: 2 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	3-4	2	1
α-helix	7-19	13
β-strand	26-35	10	1
β-strand	37-43	7	1
β-strand	48-53	6	1
α-helix	57-72	16

Chain F: 6 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	16-18	3	5
α-helix	20-23	4
β-strand	24	1	6
β-strand	41	1	6
α-helix	46-53	8
α-helix	54-56	3
β-strand	59-63	5	5
β-strand	81-85	5	5
β-strand	87	1	7
β-strand	93	1	7
α-helix	102-111	10
α-helix	112-114	3
α-helix	116-119	4

Chain S: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	51-64	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
7S RNA	A	RNA	299	CANIS LUPUS FAMILIARIS
SRP14	B	protein	75	CANIS LUPUS FAMILIARIS	P16255 (AlphaFold model)
Signal recognition particle subunit SRP68	C	protein	195	CANIS LUPUS FAMILIARIS	Q00004 (AlphaFold model)
Signal recognition particle 54 kda protein	D	protein	433	CANIS LUPUS FAMILIARIS	P61010 (AlphaFold model)
SRP9	E	protein	74	CANIS LUPUS FAMILIARIS	P21262 (AlphaFold model)
Signal recognition particle 9 kda protein	F	protein	107	CANIS LUPUS FAMILIARIS	J9PAS6
Signal sequence	S	protein	18	CANIS LUPUS FAMILIARIS	P32308

Sequence of entity 1 (A), FASTA

>4UE5_1 7S RNA (chains A)
GCCGGGCGCGGUGGCGCGCGCCUGUAGUCCCAGCUACUCGGGAGGCUGAGGCAGGAGGAU
CGCUUGAGCCCAGGAGUUCUGGGCUGCAGUGCGCUAUGCCGAUCGGGUGUCCGCACUAAG
UUCGGCAUCAAUAUGGUGACCUCCCGGGAGCGGGGGACCACCAGGUUGCCUAAGGAGGGG
UGAACCGGCCCAGGUCGGAAACGGAGCAGGUCAAAACUCCCGUGCUGAUCAGUAGUGGGA
UCGCGCCUGUGAAUAGCCACUGCACUCCAGCCUGUGCAACAUAGCGAGACCCCGUCUCU

Sequence of entity 2 (B), FASTA

>4UE5_2 SRP14 (chains B)
VLLESEQFLTELTRLFQKCRLSGSVFITLKKYDNKCLLRATDGKKKISTVVSSKEVNKFQ
MAYSNLLRANMDGLK

Sequence of entity 3 (C), FASTA

>4UE5_3 SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68 (chains C)
LSLEILQIIKESQQQHGLRHGDFQRYRGYCSRRQRRLRKTLNFKMGNRHKFTGKKVTEDL
LTDNRYLLLVLMDAERAWSYAMQLKQEANTEPRKRFHLLSRLRKAVKHAEELERLCESNR
VDAKTKLEAQAYTAYLSGMLRFEHQEWKAAIEAFNKCKTIYEKLASAFTEEQAVLYNQRV
EEISPNIRYCAYNIG

Sequence of entity 4 (D), FASTA

>4UE5_4 SIGNAL RECOGNITION PARTICLE 54 KDA PROTEIN (chains D)
MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAI
DLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSK
LAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFK
NENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKV
DVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDI
EGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFM
SKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQEL
LTQYTKFAQMVKK

Sequence of entity 5 (E), FASTA

>4UE5_5 SRP9 (chains E)
AQYQTWEEFSRAAEKLYLADPMKARVVLKYRHSDGSLCIKVTDDLVCLVYRTDQAQDVKK
IEKFHSQLMRLMVA

Sequence of entity 6 (F), FASTA

>4UE5_6 SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN (chains F)
RFICIYPAYLNNKKTIAEGRRIPISKAVENPTATEIQDVCSAVGLNVFLEKNKMYSREWN
RDVQYRGRVRVQLKQEDGSLCLVQFPSRKSVMLYAAEMIPKLKTRTQ

Sequence of entity 7 (S), FASTA

>4UE5_7 SIGNAL SEQUENCE (chains S)
LGFPINFLTLYVTVQHKK

Primary citation

Translational Arrest by a Prokaryotic Signal Recognition Particle is Mediated by RNA Interactions. Beckert, B., Kedrov, A., Sohmen, D. et al. Nat Struct Mol Biol (2015) 22:767. DOI 10.1038/NSMB.3086 · PubMed

Other PDB entries of the same protein (UniProt P16255 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7OBR 2.8 Å, RNC-SRP early complex
6FRK 3.7 Å, Structure of a prehandover mammalian ribosomal SRP and SRP receptor targeting complex
6R6G 3.7 Å, Structure of XBP1u-paused ribosome nascent chain complex with SRP.

Browse structure collections

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