4XWJ: Regulator of sigma D

Histidine-containing phosphocarrier protein (HPr) and antisigma factor Rsd complex. Determined by X-ray diffraction at 2.1 Å resolution. Released 21 Oct 2015.

Method: X-ray diffraction
Resolution: 2.1 Å
Organism: Escherichia coli (strain K12)
Chains: 2
Atoms: 1,879
Mol. weight: 28.66 kDa
Released: 21 Oct 2015

Explore 4XWJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4XWJ contains 14 α-helices and 4 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	0-12	13
α-helix	17-37	21
α-helix	39-41	3
α-helix	46-49	4
α-helix	51-67	17
α-helix	68-72	5
α-helix	73-79	7
α-helix	83-106	24
α-helix	107-111	5
α-helix	112-117	6
α-helix	120-150	31

Chain B: 3 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	2-7	6	1
α-helix	16-27	12
β-strand	32-37	6	1
β-strand	40-43	4	1
α-helix	47-50	4
β-strand	60-66	7	1
α-helix	70-80	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Regulator of sigma D	A	protein	167	Escherichia coli (strain K12)	P0AFX4 (AlphaFold model)
Phosphocarrier protein HPr	B	protein	85	Escherichia coli (strain K12)	P0AA04 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4XWJ_1 Regulator of sigma D (chains A)
MSYYHHHHHHDYDITSMLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQIM
DYYDSSLETAIDHDNYLEFQQVLSDIGESLEARFVLEDKLILLVLDA

Sequence of entity 2 (B), FASTA

>4XWJ_2 Phosphocarrier protein HPr (chains B)
MSQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASAKSLFKLQTLGLTQGTV
VTISAEGEDEQKAVEHLVKLMAELE

Primary citation

Structural basis for the sequestration of the anti-sigma (70) factor Rsd from sigma (70) by the histidine-containing phosphocarrier protein HPr. Park, Y.H., Um, S.H., Song, S. et al. Acta Crystallogr D Biol Crystallogr (2015) 71:1998-2008. DOI 10.1107/S1399004715013759 · PubMed

Other PDB entries of the same protein (UniProt P0AFX4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2P7V 2.6 Å, Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with…

Browse structure collections

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