4ZJ7: Karyopherin Kap121p

Crystal structure of the karyopherin Kap121p bound to the extreme C-terminus of the protein phosphatase Cdc14p. Determined by X-ray diffraction at 2.4 Å resolution. Released 3 Jun 2015.

Method: X-ray diffraction
Resolution: 2.4 Å
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Chains: 2
Atoms: 7,947
Mol. weight: 123.74 kDa
Released: 3 Jun 2015

Explore 4ZJ7 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4ZJ7 contains 71 α-helices and 3 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 71 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	6-18	13
α-helix	26-32	7
α-helix	33-37	5
α-helix	44-57	14
α-helix	61-77	17
α-helix	91-93	3
α-helix	96-111	16
α-helix	116-127	12
α-helix	135-136	2
α-helix	138-147	10
α-helix	153-165	13
α-helix	168-172	5
α-helix	175-187	13
α-helix	191-207	17
α-helix	210-212	3
α-helix	217-219	3
α-helix	220-225	6
α-helix	228-232	5
α-helix	236-252	17
α-helix	254-260	7
α-helix	261-273	13
α-helix	279-295	17
α-helix	297-301	5
α-helix	304-317	14
α-helix	343-359	17
α-helix	361-375	15
α-helix	381-394	14
α-helix	399-402	4
α-helix	406-413	8
α-helix	414-418	5
α-helix	422-438	17
α-helix	442-456	15
α-helix	465-479	15
α-helix	484-487	4
α-helix	488-490	3
α-helix	491-501	11
α-helix	507-524	18
α-helix	525-531	7
α-helix	532-544	13
α-helix	551-568	18
α-helix	570-573	4
α-helix	577-588	12
α-helix	597-613	17
α-helix	614-620	7
α-helix	621-631	11
β-strand	638-640	3	1
β-strand	655-659	5	1
β-strand	664-668	5	1
α-helix	669-689	21
α-helix	690-693	4
α-helix	694-700	7
α-helix	701-705	5
α-helix	707-710	4
α-helix	715-735	21
α-helix	741-758	18
α-helix	764-781	18
α-helix	788-809	22
α-helix	829-849	21
α-helix	854-857	4
α-helix	858-860	3
α-helix	861-868	8
α-helix	873-887	15
α-helix	902-909	8
α-helix	914-930	17
α-helix	941-944	4
α-helix	945-950	6
α-helix	961-977	17
α-helix	988-993	6
α-helix	1002-1014	13
α-helix	1029-1041	13
α-helix	1053-1062	10
α-helix	1066-1070	5
α-helix	1071-1073	3
α-helix	1078-1085	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Importin subunit beta-3	A	protein	1078	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P32337 (AlphaFold model)
Tyrosine-protein phosphatase CDC14	B	protein	35	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q00684 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4ZJ7_1 Importin subunit beta-3 (chains A)
MSALPEEVNRTLLQIVQAFASPDNQIRSVAEKALSEEWITENNIEYLLTFLAEQAAFSQD
TTVAALSAVLFRKLALKAPITHIRKEVLAQIRSSLLKGFLSERADSIRHKLSDAIAECVQ
DDLPAWPELLQALIESLKSGNPNFRESSFRILTTVPYLITAVDINSILPIFQSGFTDASD
NVKIAAVTAFVGYFKQLPKSEWSKLGILLPSLLNSLPRFLDDGKDDALASVFESLIELVE
LAPKLFKDMFDQIIQFTDMVIKNKDLEPPARTTALELLTVFSENAPQMCKSNQNYGQTLV
MVTLIMMTEVSIDDDDAAEWIESDDTDDEEEVTYDHARQALDRVALKLGGEYLAAPLFQY
LQQMITSTEWRERFAAMMALSSAAEGCADVLIGEIPKILDMVIPLINDPHPRVQYGCCNV
LGQISTDFSPFIQRTAHDRILPALISKLTSECTSRVQTHAAAALVNFSEFASKDILEPYL
DSLLTNLLVLLQSNKLYVQEQALTTIAFIAEAAKNKFIKYYDTLMPLLLNVLKVNNKDNS
VLKGKCMECATLIGFAVGKEKFHEHSQELISILVALQNSDIDEDDALRSYLEQSWSRICR
ILGDDFVPLLPIVIPPLLITAKATQDVGLIEEEEAANFQQYPDWDVVQVQGKHIAIHTSV
LDDKVSAMELLQSYATLLRGQFAVYVKEVMEEIALPSLDFYLHDGVRAAGATLIPILLSC
LLAATGTQNEELVLLWHKASSKLIGGLMSEPMPEITQVYHNSLVNGIKVMGDNCLSEDQL
AAFTKGVSANLTDTYERMQDRHGDGDEYNENIDEEEDFTDEDLLDEINKSIAAVLKTTNG
HYLKNLENIWPMINTFLLDNEPILVIFALVVIGDLIQYGGEQTASMKNAFIPKVTECLIS
PDARIRQAASYIIGVCAQYAPSTYADVCIPTLDTLVQIVDFPGSKLEENRSSTENASAAI
AKILYAYNSNIPNVDTYTANWFKTLPTITDKEAASFNYQFLSQLIENNSPIVCAQSNISA
VVDSVIQALNERSLTEREGQTVISSVKKLLGFLPSSDAMAIFNRYPADIMEKVHKWFA

Sequence of entity 2 (B), FASTA

>4ZJ7_2 Tyrosine-protein phosphatase CDC14 (chains B)
TILRQLLPKNRRVTSGRRTTSAAGGIRKISGSIKK

Primary citation

Crystal structure of the karyopherin Kap121p bound to the extreme C-terminus of the protein phosphatase Cdc14p. Kobayashi, J., Hirano, H., Matsuura, Y. Biochem Biophys Res Commun (2015) 463:309-314. DOI 10.1016/j.bbrc.2015.05.060 · PubMed

Other PDB entries of the same protein (UniProt P32337 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3W3W 2.2 Å, Crystal structure of Kap121p bound to Ste12p
3W3U 2.6 Å, Crystal structure of Kap121p mutant R349A/Q350A/D353A/E396A/N430K/D438A/N477A
3W3Z 2.7 Å, Crystal structure of Kap121p bound to RanGTP
3W3Y 2.8 Å, Crystal structure of Kap121p bound to Nup53p
5H2V 2.8 Å, Crystal structure of the karyopherin Kap121p bound to the SUMO protease Ulp1p
3W3T 2.9 Å, Crystal structure of Kap121p
3W3X 2.9 Å, Crystal structure of Kap121p bound to Pho4p
3W3V 3.2 Å, Crystal structure of Kap121p mutant D353K/E396K/D438K

Browse structure collections

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