4ZLK: Mouse myosin-5a

Crystal structure of mouse myosin-5a in complex with calcium-bound calmodulin. Determined by X-ray diffraction at 2.5 Å resolution. Released 4 May 2016.

Method: X-ray diffraction
Resolution: 2.5 Å
Organism: Mus musculus
Chains: 2
Atoms: 7,289
Mol. weight: 113.84 kDa
Ligands: CA
Released: 4 May 2016

Explore 4ZLK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4ZLK contains 44 α-helices and 31 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 36 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	12-17	6	1
β-strand	21-27	7	1
α-helix	30-32	3
β-strand	37-41	5	1
β-strand	47-51	5	1
α-helix	59-60	2
β-strand	61-62	2	1
α-helix	63-65	3
β-strand	73	1	2
α-helix	74-76	3
α-helix	82-94	13
β-strand	100-103	4	2
β-strand	106-110	5	2
α-helix	121-127	7
α-helix	130-131	2
α-helix	139-153	15
β-strand	157-162	6	2
α-helix	169-183	15
α-helix	192-207	16
β-strand	208-209	2	3
β-strand	217-218	2	3
β-strand	221-228	8	2
β-strand	234-242	9	2
α-helix	246-249	4
β-strand	259	1	3
α-helix	260-266	7
α-helix	272-277	6
α-helix	282-284	3
α-helix	286-289	4
α-helix	301-314	14
α-helix	319-335	17
α-helix	338-340	3
β-strand	341-342	2	4
β-strand	348-349	2	4
α-helix	355-363	9
α-helix	368-376	9
β-strand	377-380	4	5
β-strand	387-390	4	5
α-helix	393-421	29
β-strand	431-438	8	2
β-strand	447	1	6
α-helix	449-479	31
α-helix	493-500	8
α-helix	505-513	9
α-helix	520-531	12
β-strand	538-539	2	6
β-strand	547-551	5	6
β-strand	556-560	5	6
α-helix	564-568	5
α-helix	574-581	8
α-helix	587-591	5
α-helix	635-651	17
β-strand	654-661	8	2
α-helix	674-683	10
α-helix	686-693	8
β-strand	699-702	4	7
α-helix	703-710	8
α-helix	711-713	3
α-helix	723-734	12
α-helix	738-740	3
β-strand	741-743	3	7
β-strand	747-750	4	7
α-helix	754-786	33

Chain B: 8 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	8-19	12
β-strand	26-27	2	8
α-helix	29-38	10
α-helix	45-54	10
β-strand	63-64	2	8
α-helix	65-72	8
α-helix	82-92	11
β-strand	99-100	2	9
α-helix	102-111	10
α-helix	118-128	11
β-strand	136-137	2	9
α-helix	138-145	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Unconventional myosin-Va	A	protein	839	Mus musculus	Q99104 (AlphaFold model)
Calmodulin	B	protein	149	Mus musculus	P0DP26 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4ZLK_1 Unconventional myosin-Va (chains A)
MSYYHHHHHHDYKDDDDKNIPTTENLYFQGAMGIRNSKAYVDELTSPAMAASELYTKFAR
VWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGEN
DLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNM
GDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANV
EEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQ
AEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQAC
TLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMC
HWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSF
IGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDN
QPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKH
FADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPL
TRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTF
DEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLE
KLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQR

Sequence of entity 2 (B), FASTA

>4ZLK_2 Calmodulin (chains B)
MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE
EVDEMIREADIDGDGQVNYEEFVQMMTAK

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	4

Primary citation

Structural basis for calcium regulation of myosin 5 motor function. Shen, M., Zheng, S., Zhang, W. et al. To be published.

Other PDB entries of the same protein (UniProt Q99104 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6KU0 1.6 Å, Crystal structure of MyoVa-GTD in complex with MICAL1-GTBM
4KP3 2.4 Å, Crystal Structure of MyoVa-GTD in Complex with Two Cargos
3WB8 2.5 Å, Crystal Structure of MyoVa-GTD
2IX7 2.5 Å, Structure of apo-calmodulin bound to unconventional myosin V
8RBF 4.2 Å, CryoEM structure of the post-powerstroke actomyosin-5a complex
8R9V 4.4 Å, CryoEM structure of the primed actomyosin-5a complex
7YV9 4.78 Å, Cryo-EM structure of full-length Myosin Va in the autoinhibited state
8RBG 4.9 Å, CryoEM structure of primed myosin-5a (ADP-Pi state)
8OF8 7.5 Å, Cryo-EM structure of actomyosin-5a-S1 with the full-length lever (nucleotide free)

Browse structure collections

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