5A5T: PDB entry 5A5T
Structure of mammalian eIF3 in the context of the 43S preinitiation complex. Determined by electron microscopy at 6.0 Å resolution. Released 9 Sept 2015.
- Method
- Electron microscopy
- Resolution
- 6.0 Å
- Organism
- ORYCTOLAGUS CUNICULUS
- Chains
- 8
- Atoms
- 25,432
- Mol. weight
- 527.4 kDa
- Released
- 9 Sept 2015
Explore 5A5T in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
5A5T contains 163 α-helices and 39 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 32 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-20 | 13 | |
| α-helix | 24-36 | 13 | |
| α-helix | 47-62 | 16 | |
| α-helix | 67-76 | 10 | |
| α-helix | 82-116 | 35 | |
| α-helix | 119-121 | 3 | |
| α-helix | 123-131 | 9 | |
| α-helix | 139-162 | 24 | |
| α-helix | 167-168 | 2 | |
| α-helix | 169-173 | 5 | |
| α-helix | 174-187 | 14 | |
| α-helix | 190-208 | 19 | |
| α-helix | 216-218 | 3 | |
| α-helix | 221-238 | 18 | |
| α-helix | 242-263 | 22 | |
| α-helix | 267-287 | 21 | |
| α-helix | 289-310 | 22 | |
| α-helix | 315-328 | 14 | |
| α-helix | 333-335 | 3 | |
| α-helix | 338-342 | 5 | |
| α-helix | 345-359 | 15 | |
| α-helix | 364-366 | 3 | |
| α-helix | 367-376 | 10 | |
| α-helix | 379-382 | 4 | |
| α-helix | 385-395 | 11 | |
| α-helix | 403-413 | 11 | |
| α-helix | 422-446 | 25 | |
| β-strand | 447 | 1 | 1 |
| β-strand | 449-451 | 3 | 2 |
| α-helix | 452-458 | 7 | |
| α-helix | 464-477 | 14 | |
| β-strand | 483 | 1 | 2 |
| β-strand | 490-492 | 3 | 2 |
| α-helix | 501-504 | 4 | |
| α-helix | 523-537 | 15 | |
| α-helix | 541-601 | 61 | |
Chain C: 29 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 327-340 | 14 | |
| α-helix | 347-360 | 14 | |
| α-helix | 366-385 | 20 | |
| α-helix | 386-388 | 3 | |
| α-helix | 395-414 | 20 | |
| α-helix | 437-461 | 25 | |
| α-helix | 467-487 | 21 | |
| α-helix | 492-508 | 17 | |
| α-helix | 515-523 | 9 | |
| α-helix | 535-555 | 21 | |
| α-helix | 562-575 | 14 | |
| α-helix | 576-580 | 5 | |
| α-helix | 582-592 | 11 | |
| α-helix | 595-599 | 5 | |
| α-helix | 602-622 | 21 | |
| α-helix | 626-632 | 7 | |
| α-helix | 634-637 | 4 | |
| α-helix | 642-645 | 4 | |
| α-helix | 660-666 | 7 | |
| α-helix | 673-675 | 3 | |
| α-helix | 679-700 | 22 | |
| α-helix | 701-705 | 5 | |
| α-helix | 714-722 | 9 | |
| α-helix | 733-746 | 14 | |
| α-helix | 749-762 | 14 | |
| α-helix | 772-795 | 24 | |
| β-strand | 801-803 | 3 | 3 |
| α-helix | 804-810 | 7 | |
| α-helix | 815-829 | 15 | |
| β-strand | 834-836 | 3 | 3 |
| β-strand | 841-843 | 3 | 3 |
| α-helix | 850-875 | 26 | |
Chain E: 22 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-13 | 9 | |
| α-helix | 33-45 | 13 | |
| α-helix | 52-59 | 8 | |
| α-helix | 65-69 | 5 | |
| α-helix | 77-87 | 11 | |
| α-helix | 96-121 | 26 | |
| α-helix | 124-140 | 17 | |
| α-helix | 145-154 | 10 | |
| α-helix | 161-179 | 19 | |
| α-helix | 182-195 | 14 | |
| α-helix | 204-219 | 16 | |
| α-helix | 225-234 | 10 | |
| α-helix | 253-262 | 10 | |
| α-helix | 270-272 | 3 | |
| α-helix | 273-278 | 6 | |
| α-helix | 281-287 | 7 | |
| α-helix | 291-302 | 12 | |
| α-helix | 308-319 | 12 | |
| α-helix | 329-345 | 17 | |
| β-strand | 348 | 1 | 3 |
| β-strand | 350-352 | 3 | 4 |
| α-helix | 353-360 | 8 | |
| α-helix | 364-376 | 13 | |
| β-strand | 383-384 | 2 | 4 |
| β-strand | 390-392 | 3 | 4 |
| α-helix | 401-419 | 19 | |
Chain F: 9 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 111-114 | 4 | 5 |
| α-helix | 116-127 | 12 | |
| β-strand | 137-145 | 9 | 5 |
| β-strand | 148-156 | 9 | 5 |
| β-strand | 159-162 | 4 | 6 |
| β-strand | 165-168 | 4 | 6 |
| α-helix | 170-183 | 14 | |
| β-strand | 188-195 | 8 | 5 |
| α-helix | 203-211 | 9 | |
| β-strand | 219-222 | 4 | 5 |
| α-helix | 223 | 1 | |
| β-strand | 233-239 | 7 | 5 |
| β-strand | 251-259 | 9 | 5 |
| α-helix | 263-277 | 15 | |
| α-helix | 285-313 | 29 | |
| α-helix | 318-320 | 3 | |
| α-helix | 322-334 | 13 | |
| α-helix | 341-374 | 34 | |
Chain H: 13 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 31 | 1 | 7 |
| β-strand | 37 | 1 | 7 |
| β-strand | 38-41 | 4 | 8 |
| α-helix | 43-51 | 9 | |
| β-strand | 65-71 | 7 | 8 |
| β-strand | 75-83 | 9 | 8 |
| β-strand | 87-88 | 2 | 9 |
| β-strand | 92-93 | 2 | 9 |
| α-helix | 97-110 | 14 | |
| β-strand | 116-121 | 6 | 8 |
| α-helix | 131-143 | 13 | |
| β-strand | 148-152 | 5 | 8 |
| α-helix | 155-157 | 3 | |
| β-strand | 164-167 | 4 | 8 |
| α-helix | 171-175 | 5 | |
| α-helix | 186-189 | 4 | |
| β-strand | 200 | 1 | 8 |
| α-helix | 212-218 | 7 | |
| α-helix | 226-242 | 17 | |
| α-helix | 245-272 | 28 | |
| α-helix | 273-277 | 5 | |
| α-helix | 278-289 | 12 | |
| α-helix | 299-303 | 5 | |
| α-helix | 315-350 | 36 | |
Chain K: 17 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-14 | 12 | |
| α-helix | 19-21 | 3 | |
| α-helix | 23-25 | 3 | |
| α-helix | 26-38 | 13 | |
| α-helix | 44-56 | 13 | |
| α-helix | 58-60 | 3 | |
| α-helix | 63-75 | 13 | |
| α-helix | 81-88 | 8 | |
| α-helix | 91-94 | 4 | |
| α-helix | 99-110 | 12 | |
| α-helix | 114-119 | 6 | |
| α-helix | 126-129 | 4 | |
| α-helix | 134-149 | 16 | |
| β-strand | 150 | 1 | 10 |
| β-strand | 152-153 | 2 | 11 |
| α-helix | 155-162 | 8 | |
| α-helix | 167-174 | 8 | |
| α-helix | 179 | 1 | |
| β-strand | 180-181 | 2 | 11 |
| β-strand | 187-188 | 2 | 11 |
| α-helix | 206-213 | 8 | |
Chain L: 18 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 183-204 | 22 | |
| α-helix | 209-217 | 9 | |
| α-helix | 224-246 | 23 | |
| α-helix | 253-255 | 3 | |
| α-helix | 256-262 | 7 | |
| α-helix | 263-267 | 5 | |
| α-helix | 269-271 | 3 | |
| α-helix | 275-289 | 15 | |
| α-helix | 296-301 | 6 | |
| α-helix | 309-325 | 17 | |
| α-helix | 329-341 | 13 | |
| α-helix | 357-375 | 19 | |
| α-helix | 388-401 | 14 | |
| α-helix | 406-420 | 15 | |
| α-helix | 437-460 | 24 | |
| β-strand | 469-471 | 3 | 10 |
| α-helix | 472-482 | 11 | |
| α-helix | 490-498 | 9 | |
| β-strand | 504-506 | 3 | 10 |
| β-strand | 512-515 | 4 | 10 |
| α-helix | 525-548 | 24 | |
Chain M: 23 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 15-23 | 9 | |
| α-helix | 28-38 | 11 | |
| α-helix | 45-53 | 9 | |
| α-helix | 63-72 | 10 | |
| α-helix | 79-83 | 5 | |
| α-helix | 87-93 | 7 | |
| α-helix | 97-106 | 10 | |
| α-helix | 118-130 | 13 | |
| α-helix | 133-147 | 15 | |
| α-helix | 155-171 | 17 | |
| α-helix | 175-191 | 17 | |
| α-helix | 197-213 | 17 | |
| α-helix | 221-225 | 5 | |
| α-helix | 228-230 | 3 | |
| α-helix | 240-248 | 9 | |
| α-helix | 252-258 | 7 | |
| α-helix | 261-266 | 6 | |
| α-helix | 269-288 | 20 | |
| α-helix | 294-301 | 8 | |
| α-helix | 305-318 | 14 | |
| α-helix | 324 | 1 | |
| β-strand | 325 | 1 | 1 |
| α-helix | 326 | 1 | |
| α-helix | 342-366 | 25 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Eukaryotic translation initiation factor 3 subunit A | A | protein | 1362 | ORYCTOLAGUS CUNICULUS | G1SMZ5 |
| Eukaryotic translation initiation factor 3 subunit C | C | protein | 843 | ORYCTOLAGUS CUNICULUS | G1U971 (AlphaFold model) |
| Eukaryotic translation initiation factor 3 subunit E | E | protein | 445 | ORYCTOLAGUS CUNICULUS | G1SUC8 (AlphaFold model) |
| Eukaryotic translation initiation factor 3 subunit F | F | protein | 364 | ORYCTOLAGUS CUNICULUS | U3KNL5 |
| Eukaryotic translation initiation factor 3 subunit H | H | protein | 352 | ORYCTOLAGUS CUNICULUS | G1ST95 |
| Eukaryotic translation initiation factor 3 subunit K | K | protein | 218 | ORYCTOLAGUS CUNICULUS | G1T3L2 |
| Uncharacterized protein | L | protein | 564 | ORYCTOLAGUS CUNICULUS | G1SED9 |
| Eukaryotic translation initiation factor 3 subunit M | M | protein | 374 | ORYCTOLAGUS CUNICULUS | G1SLW8 |
Sequence of entity 1 (A), FASTA
>5A5T_1 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A (chains A)
MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVD
LRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKLAEEKTEAAKEESQQMVLDIEDLDN
IQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQ
AFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSA
ISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLY
HLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLG
LQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEK
EPELQQYVPQLQSNTILRLLQQVAQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDL
QVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPA
HILQEKEEQHQLAVTAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAE
LQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIED
LEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRIK
DMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFE
ERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEE
LREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGEDPLSRRDSRWGDRDSEGTWR
KGPEADSEWRRGPPEKEWRRGEGRDDERSHRRDEERPRRVGDDEDRESSLRPDDDRTLRR
GMEDDRGPRRGPDEERFSRRGLEDERPSWRSTDDDRPPRRIGEEDRGSWRHAEDDRPPRR
GLDDDRGSWRTADEDRGPRRMMDEDRGPRRGGADDERPSWRSADDDRGPRRGIDDDRGPR
RGLDDDRGPWRNADDDRIPRRGADDDRGPWRNMDDDRMSRRADDDRISRRGEDSRPGPWR
PFVKPGGWREKEKAREESWGPPRESRPPEEREWERERDRDNPDREENDKDPERERDRERD
GDREERFRRPRDEGGWRRGPAEEASSWRDSSRRDDRDRDDRRRERDDRRDLRDRRDLRDD
RDRRGPPLRSERDEVSSWRRADDRKDDRAEERDPPRRVPPPVLSRDRDRDRDRDRDRDRE
REREREREGEKEKTSWRAEKDRESLRRTKNETDEDGWTTVRR
Sequence of entity 2 (C), FASTA
>5A5T_2 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C (chains C)
MKIRDVTKCLEEFELLGKAYGKAKSIVDKEGVPRFYIRILADLEDYLNELWEDKEGKKKM
NKNNAKALSTLRQKIRKYNRDFESHITNYKQNPEQSADEDAEKNEEDSEGSSDEDEDEDG
VSAAAFLKKKAEAPSGESRKFLKKMEDEDEDSEDSEDDEEWDTGSTSSDSDSEEEEGKQT
ALASRFLKKAPTTEEDKKAAEKKREDKAKKKHDRKSKRLDEEEEDNEGGEWERVRGGVPL
VKEKPKMFAKGTEITHAVVIKKLNEILQARGKKGTDRAAQIELLQLLVQIASENNLGEGV
IVKIKFNIIASLYDYNPNLATYMKPEMWQKCLDCINELMDILFANPNIFVGENILEESEN
LQNVDQPLRVRGCILTLVERMDEEFTKIMQNTDPHSQEYVEHLKDEAQVCAIIERVQRYL
EEKGTTEEICRVYLRRILHTYYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMER
LCKYIYAKDRTDRIRTCAILCHIYHHYLHSRWYQARDLMLMSHLQDNIQHADPPVQILYN
RTMVQLGICAFRQGLTKDAHNALLDIQSSGRAKELLGQGLLLRSLQERNQEQEKVERRRQ
VPFHLHINLELLECVYLVSAMLLEIPYMAAHESDARRRMISKQFHHQLRVGERQPLLGPP
ESMREHVVAASKAMKMGDWKTCHSFIINEKMNGKVWDLFPEADKVRTMLVRKIQEESLRT
YLFTYSSVYDSISMETLSDMFELDLPTVHSIISKMIINEELMASLDQPTQTVVMHRTEPT
AQQNLALQLAEKLGSLVENNERVFDHKQGTYGGYFRDQKDGYRKNEGYMRRGGYRQQQSQ
TAY
Sequence of entity 3 (E), FASTA
>5A5T_3 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E (chains E)
MAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDLLSDTNMVDFAMDVYKN
LYSDDIPHALREKRTTVVAQLKQLQAETEPIVKMFEDPETTRQMQSTRDGRMLFDYLADK
HGFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQ
NWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQP
QYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLY
VNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKL
NMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAM
NIEKKLNQNSRSEAPNWATQDSGFY
Sequence of entity 4 (F), FASTA
>5A5T_4 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F (chains F)
MATPAVSASAPPATPAATPAAAAAPASAPAAAPAPTPAPAPAAAPAAGAAPAAAADPAAA
AAAAGTGQTPASAQAPAQTSAPSLPGPALPGPFPGGRVVRLHPVILASIVDSYERRNEGA
ARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWY
ATGHDITEHSVLIHEYYSREAPNPIHLTVDTSLQNGRMSIKAYVSTSMGVPGRTMGVMFT
PLTVKYAYYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDV
LSGKVSADNTVGRFLMSLVNQVPKIVPDDFETMLNSNINDLLMVTYLANLTQSQIALNEK
LVNL
Sequence of entity 5 (H), FASTA
>5A5T_5 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT H (chains H)
MASRKEGTGSGGTSSSSATGVVGKGKGKGGSGDSAVKQVQIDGLVVLKIIKHYQEEGQGT
EVVQGVLLGLVVEDRLEITNCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWY
QSTYYGSFVTRALLDSQFSYQHAIEESVVLIYDPIKTAQGSLSLKAYRLTPKLMEVCKEK
DFSPEALKKANITFEHMFEEVPIVIKNSHLINVLMWELEKKSAVADKHELLSLASSNHLG
KNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEED
LSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGKLFMAQALQEYNN
Sequence of entity 6 (K), FASTA
>5A5T_6 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT K (chains K)
MAMFEQMRANVGKLLKGIDRYNPENLATLERYVETQAKENAYDLEANLAVLKLYQFNPAF
FQTTVTAQILLKALTNLPHTDFTLCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQA
LDENMDLLEGITGFEDSVRKFICHVVGITYQHIDRWLLAEMLGDLTDSQLKMWMSKYGWS
ADESGQIFICSQEESIKPKNIVEKIDFDSVSSIMASSQ
Sequence of entity 7 (L), FASTA
>5A5T_7 UNCHARACTERIZED PROTEIN (chains L)
MSYPADDYESEAVYDPYAYPSDYDMHTGDPKQDLAYERQYEQQTYQVIPEVIRNFIQYFH
KTVSDLIDQKVYELQASRVSSDVIDQKVYEIQDIYENSWTKLTERFFKNTPWPEAETIAP
QVGNDAVFLILYKELYYRHIYAKVSGGPSLAQRFESYYNYCNLFNYILNADGPAPLELPN
QWLWDIIDEFIYQFQSFSQYRCKTAKKSEEEIDFLRSNPKIWNVHSVLNVLHSLVDKSNI
NRQLEVYTSGGDPESVAGEYGRHSLYKMLGYFSLVGLLRLHSLLGDYYQAIKVLENIELN
KKSMYSRVPECQVTTYYYVGFAYLMMRRYQDAIRVFANILLYIQRTKSMFQRTTYKYEMI
NKQNEQMHALLAIALTMYPMRIDESIHLQLREKYGDKMLRMQKGDPQVYEELFSYSCPKF
LSPVVPNYDSVHPNYHKEPFLQQLKVFSDEVQQQAQLSTIRSFLKLYTTMPVAKLAGFLD
LTEQEFRIQLLVFKHKMKNLVWTSGISALDGEFQSASEVDFYIDKDMIHIADTKVARRYG
DFFIRQIHKFEELNRTLKKMGQRP
Sequence of entity 8 (M), FASTA
>5A5T_8 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT M (chains M)
MSVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACDVCLKEDDKDV
ESVMNSVVSLLLILEPDKQEALIESLCEKLVKFREGERPSLRLQLLSNLFHGMDKNTPVR
YTVYCSLIKVAASCGAIQYIPTELDQVRKWISDWNLTTEKKHTLLRLLYEALVDCKKSDA
ASKVMVELLGSYTEDNASQARVDAHRCIVRALKDPNAFLFDHLLTLKPVKFLEGELIHDL
LTIFVSAKLASYVKFYQNNKDFIDSLGLLHEQNMAKMRLLTFMGMAVENKEISFDTMQQE
LQIGADDVEAFVIDAVRTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQLYDTLNAWKQN
LNKVKNSLLSLSDT
Primary citation
Structure of Mammalian Eif3 in the Context of the 43S Preinitiation Complex. Des-Georges, A., Dhote, V., Kuhn, L. et al. Nature (2015) 525:491. DOI 10.1038/NATURE14891 · PubMed
Other PDB entries of the same protein (UniProt G1SMZ5), best resolution first:
- 9H74 2.9 Å, Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA
- 8SUP 3.1 Å, Structure of the 48S translation initiation complex assembled on the…
- 6W2T 3.36 Å, Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small…
- 6W2S 3.47 Å, Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small…
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