5AER: Neuronal calcium sensor 1

Neuronal calcium sensor-1 (NCS-1)from Rattus norvegicus complex with D2 dopamine receptor peptide from Homo sapiens. Determined by X-ray diffraction at 2.19 Å resolution. Released 18 Feb 2015.

Method: X-ray diffraction
Resolution: 2.19 Å
Organisms: RATTUS NORVEGICUS, HOMO SAPIENS
Chains: 3
Atoms: 1,754
Mol. weight: 25.5 kDa
Ligands: CA
Released: 18 Feb 2015

Explore 5AER in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5AER contains 15 α-helices and 4 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	11-19	9
α-helix	24-37	14
β-strand	42-43	2	1
α-helix	45-55	11
α-helix	62-72	11
β-strand	80-81	2	1
α-helix	82-94	13
α-helix	97-108	12
β-strand	116-117	2	2
α-helix	118-132	15
α-helix	133-135	3
α-helix	140-142	3
α-helix	145-156	12
β-strand	163-164	2	2
α-helix	166-175	10
α-helix	177-180	4
α-helix	186-189	4

Chain B: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	431-442	12

Chain C: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	432-440	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Neuronal calcium sensor 1	A	protein	190	RATTUS NORVEGICUS	P62168 (AlphaFold model)
D(2) dopamine receptor	B, C	protein	14	HOMO SAPIENS	P14416 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5AER_1 NEURONAL CALCIUM SENSOR 1 (chains A)
MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGD
PTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNE
MLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIV
QALSLYDGLV

Sequence of entity 2 (B, C), FASTA

>5AER_2 D(2) DOPAMINE RECEPTOR (chains B, C)
NIEFRKAFLKILHS

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	3

Water and common crystallization additives (K) are not listed.

Primary citation

Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-Protein-Coupled Receptor Kinase 1 (Grk1) Peptides Using Different Modes of Interactions. Pandalaneni, S., Karuppiah, V., Saleem, M. et al. J Biol Chem (2015) 290:18744. DOI 10.1074/JBC.M114.627059 · PubMed

Other PDB entries of the same protein (UniProt P62168 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5AEQ 1.95 Å, Neuronal calcium sensor (NCS-1)from Rattus norvegicus
5AFP 2.3 Å, Neuronal calcium sensor-1 (NCS-1)from Rattus norvegicus complex with rhodopsin kinase…
4OV2 2.6 Å, Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus…
4YRU 2.8 Å, Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus…

Browse structure collections

About this viewer

MolViewer shows 5AER directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.