5AJK: Variola virus virulence factor F1L

Name: PDB 5AJK: Crystal structure of variola virus virulence factor F1L in complex with human Bak BH3 domain
Published: 2015-03-25
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: apoptosis

Crystal structure of variola virus virulence factor F1L in complex with human Bak BH3 domain. Determined by X-ray diffraction at 2.55 Å resolution. Released 25 Mar 2015.

Method: X-ray diffraction
Resolution: 2.55 Å
Organisms: VARIOLA VIRUS, HOMO SAPIENS
Chains: 12
Atoms: 8,055
Mol. weight: 135.45 kDa
Released: 25 Mar 2015

Explore 5AJK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5AJK contains 55 α-helices and 2 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	50-53	4
α-helix	66-69	4
α-helix	73-96	24
α-helix	99-118	20
α-helix	120-130	11
β-strand	133	1	1
α-helix	137-149	13
α-helix	155-170	16
α-helix	174-188	15
α-helix	191-200	10

Chains B and D: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	69-88	20

Chain C: 8 helices, 0 β-strands

Element	Residues	Length
α-helix	66-69	4
α-helix	73-96	24
α-helix	99-118	20
α-helix	120-130	11
α-helix	137-150	14
α-helix	154-170	17
α-helix	174-188	15
α-helix	191-199	9

Chain E: 8 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	67-69	3
α-helix	73-95	23
α-helix	99-118	20
α-helix	120-131	12
β-strand	133	1	1
α-helix	137-150	14
α-helix	155-170	16
α-helix	174-188	15
α-helix	191-200	10

Chain F: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	69-89	21

Chain G: 8 helices, 0 β-strands

Element	Residues	Length
α-helix	66-68	3
α-helix	73-96	24
α-helix	99-118	20
α-helix	120-130	11
α-helix	137-149	13
α-helix	154-170	17
α-helix	174-188	15
α-helix	191-199	9

Chains H and L: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	70-89	20

Chain I: 8 helices, 0 β-strands

Element	Residues	Length
α-helix	66-69	4
α-helix	73-96	24
α-helix	99-118	20
α-helix	120-131	12
α-helix	137-150	14
α-helix	154-170	17
α-helix	174-188	15
α-helix	191-199	9

2 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Homolog of vaccinia virus cds F1L	A, C, E, G, I, K	protein	168	VARIOLA VIRUS	Q85365
Bcl-2 homologous antagonist/killer	B, D, F, H, J, L	protein	26	HOMO SAPIENS	Q16611 (AlphaFold model)

Sequence of entity 1 (A, C, E, G, I, K), FASTA

>5AJK_1 HOMOLOG OF VACCINIA VIRUS CDS F1L (chains A, C, E, G, I, K)
GPLGSKASNNDDHNYVYPLPENMVYRFNKSTNILDYLSTERDHVMMAVQYYMSKQRLDDL
YRQLPTKTRSYIDIINMYCDKVNNDYNRDMNIMYDMASTESFTVYDINNEVNTILMDNKG
LGVRLATISFITELGKRCMNPVETIKMFTLLSHTICDDCFIDYITDIS

Sequence of entity 2 (B, D, F, H, J, L), FASTA

>5AJK_2 BCL-2 HOMOLOGOUS ANTAGONIST/KILLER (chains B, D, F, H, J, L)
PSSTMGQVGRQLAIIGDDINRRYDSE

Primary citation

Variola Virus F1L is a Bcl-2-Like Protein that Unlike its Vaccinia Virus Counterpart Inhibits Apoptosis Independent of Bim. Marshall, B., Puthalakath, H., Caria, S. et al. Cell Death Dis (2015) 6:E1680. DOI 10.1038/CDDIS.2015.52 · PubMed

Other PDB entries of the same protein (UniProt Q85365), best resolution first:

5AJJ 1.75 Å, Crystal structure of variola virus virulence factor F1L in complex with human Bid BH3…

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