5B03: MoeN5-Sso7d fusion protein

Structure of MoeN5-Sso7d fusion protein in complex with geranyl pyrophosphate. Determined by X-ray diffraction at 2.6 Å resolution. Released 23 Mar 2016.

Method: X-ray diffraction
Resolution: 2.6 Å
Organisms: Streptomyces ghanaensis, Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Chains: 4
Atoms: 9,801
Mol. weight: 151.58 kDa
Ligands: GPP
Released: 23 Mar 2016

Explore 5B03 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5B03 contains 68 α-helices and 14 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 0 β-strands

Element	Residues	Length
α-helix	-3-18	22
α-helix	23-30	8
α-helix	33-42	10
α-helix	49-74	26
α-helix	81-100	20
α-helix	107-115	9
α-helix	117-129	13
α-helix	135-142	8
α-helix	143-148	6
α-helix	149-160	12
α-helix	167-192	26
α-helix	200-206	7
α-helix	211-228	18
α-helix	238-248	11
α-helix	249-253	5
α-helix	254-256	3

Chain B: 18 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	-2-18	21
α-helix	23-31	9
α-helix	33-42	10
α-helix	49-74	26
α-helix	81-102	22
α-helix	108-115	8
α-helix	117-129	13
α-helix	135-142	8
α-helix	143-148	6
α-helix	149-160	12
α-helix	167-192	26
α-helix	200-205	6
α-helix	211-230	20
α-helix	238-248	11
α-helix	249-253	5
α-helix	255-258	4
β-strand	268-271	4	1
β-strand	278-281	4	1
β-strand	285-291	7	2
β-strand	294-302	9	2
β-strand	305-312	8	2
α-helix	313-315	3
α-helix	318-325	8

Chain C: 16 helices, 0 β-strands

Element	Residues	Length
α-helix	-1-19	21
α-helix	23-30	8
α-helix	33-42	10
α-helix	49-74	26
α-helix	81-102	22
α-helix	107-115	9
α-helix	117-129	13
α-helix	135-142	8
α-helix	143-148	6
α-helix	149-161	13
α-helix	167-192	26
α-helix	200-205	6
α-helix	211-230	20
α-helix	238-248	11
α-helix	249-253	5
α-helix	254-257	4

Chain D: 18 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	-2-18	21
α-helix	23-30	8
α-helix	33-42	10
α-helix	49-74	26
α-helix	81-100	20
α-helix	108-115	8
α-helix	117-129	13
α-helix	135-142	8
α-helix	143-148	6
α-helix	149-160	12
α-helix	167-192	26
α-helix	200-205	6
α-helix	211-230	20
α-helix	238-248	11
α-helix	249-253	5
β-strand	268-270	3	3
β-strand	273	1	4
β-strand	276	1	4
β-strand	279-281	3	3
β-strand	287-291	5	5
β-strand	294-298	5	5
β-strand	300-301	2	6
β-strand	305-306	2	6
α-helix	307	1
β-strand	309-312	4	5
α-helix	313-315	3
α-helix	318-326	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
MoeN5,DNA-binding protein 7d	A, B, C, D	protein	343	Streptomyces ghanaensis, Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	A0A010 (AlphaFold model), P39476 (AlphaFold model)

Sequence of entity 1 (A, B, C, D), FASTA

>5B03_1 MoeN5,DNA-binding protein 7d (chains A, B, C, D)
MAHHHHHHVDDDDKMLAAEAANRDHVTRCVAQTGGSPDLVAHTAALRLYLRVPHFLTEWT
TDPDRRAAVSRALALDIVSMKLLDDLMDDDTGLDRVELACVCLRLHLRALHELESLARDP
KAVTDILEQDAVHLCGGQIRTKRSRATNLREWRAHASTYGSTFLGRYGALAAACGGEGQP
ADSVREFAEAFAMTITMADDLTDYDRNGERDGNLAHLMRTGAVAGQDVVDLLEELRGRAL
AAVAAPPGAPGLVPVVHLYTDDVLVRLLPRHLGEAGAGAMATVKFKYKGEEKEVDISKIK
KVWRVGKMISFTYDEGGGKTGRGAVSEKDAPKELLQMLEKQKK

Ligands and cofactors

ID	Name	Formula	Copies
GPP	Geranyl diphosphate	C10 H20 O7 P2	4

Primary citation

Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5. Zhang, L., Chen, C.C., Ko, T.P. et al. Angew Chem Int Ed Engl (2016) 55:4716-4720. DOI 10.1002/anie.201511388 · PubMed

Other PDB entries of the same protein (UniProt A0A010 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5B02 2.21 Å, Structure of the prenyltransferase MoeN5 with a fusion protein tag of Sso7d
6J8V 2.23 Å, Structure of MOEN5-SSO7D fusion protein in complex with ligand 2
5B0I 2.26 Å, Structure of MoeN5-Sso7d fusion protein in complex with beta-octyl glucoside
5GWW 2.3 Å, Structure of MoeN5-Sso7d fusion protein in complex with a permethylated substrate analogue
6J8W 2.35 Å, Structure of MOEN5-SSO7D fusion protein in complex with lig 1
5GWV 2.4 Å, Structure of MoeN5-Sso7d fusion protein in complex with a substrate analogue
5B0J 2.5 Å, Structure of MoeN5-Sso7d fusion protein in complex with beta-undecyl maltoside
5B0K 2.75 Å, Structure of MoeN5-Sso7d fusion protein in complex with beta-decyl maltoside
5B0L 2.8 Å, Structure of MoeN5-Sso7d fusion protein in complex with beta-nonyl glucoside
5B00 2.95 Å, Structure of the prenyltransferase MoeN5 in complex with geranyl pyrophosphate
5B0M 3.05 Å, Structure of MoeN5-Sso7d fusion protein in complex with beta-dodecyl maltoside
5B01 3.45 Å, Structure of a prenyltransferase in its unbound form

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