5EZ0: PTPN4 PDZ domain

Crystal structure of the PTPN4 PDZ domain complexed with the PDZ binding motif of the mitogen activated protein kinase P38GAMMA. Determined by X-ray diffraction at 2.35 Å resolution. Released 8 Jun 2016.

Method: X-ray diffraction
Resolution: 2.35 Å
Organism: Homo sapiens
Chains: 8
Atoms: 3,204
Mol. weight: 52.07 kDa
Released: 8 Jun 2016

Explore 5EZ0 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5EZ0 contains 17 α-helices and 34 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	516-520	5	1
β-strand	530-535	6	1
β-strand	540-547	8	1
α-helix	552-555	4
α-helix	559-561	3
α-helix	564	1
β-strand	565-569	5	1
β-strand	572-573	2	1
α-helix	579-586	8
β-strand	597-602	6	1

Chain B: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	515	1	2
β-strand	516-520	5	3
β-strand	530-533	4	3
β-strand	543-547	5	3
α-helix	552-555	4
α-helix	564	1
β-strand	565-569	5	3
β-strand	572-573	2	3
α-helix	579-586	8
β-strand	597-602	6	3

Chain C: 5 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	516-520	5	4
β-strand	530-533	4	4
β-strand	543-547	5	4
α-helix	552-555	4
α-helix	559-560	2
α-helix	564	1
β-strand	565-569	5	4
β-strand	572-573	2	4
α-helix	579-587	9
α-helix	589-591	3
β-strand	593	1	5
β-strand	596	1	5
β-strand	597-602	6	4

Chain D: 5 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	515	1	2
β-strand	516-520	5	6
β-strand	530-533	4	6
β-strand	543-547	5	6
α-helix	552-555	4
α-helix	559-560	2
α-helix	564	1
β-strand	565-569	5	6
β-strand	572-573	2	6
α-helix	579-587	9
α-helix	589-591	3
β-strand	593	1	7
β-strand	596	1	7
β-strand	597-602	6	6

Chains E, F, G and H: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	10-12	3	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Tyrosine-protein phosphatase non-receptor type 4	A, B, C, D	protein	107	Homo sapiens	P29074 (AlphaFold model)
Mitogen-activated protein kinase 12	E, F, G, H	protein	11	Homo sapiens	B5MDL5 (AlphaFold model)

Sequence of entity 1 (A, B, C, D), FASTA

>5EZ0_1 Tyrosine-protein phosphatase non-receptor type 4 (chains A, B, C, D)
GSSPEKPTPNGGIPHDNLVLIRMKPDENGRFGFNVKGGYDQKMPVIVSRVAPGTPADLCV
PRLNEGDQVVLINGRDIAEHTHDQVVLFIKASCERHSGELMLLVRPN

Sequence of entity 2 (E, F, G, H), FASTA

>5EZ0_2 Mitogen-activated protein kinase 12 (chains E, F, G, H)
SWARVSKETPL

Primary citation

Molecular Basis of the Interaction of the Human Protein Tyrosine Phosphatase Non-receptor Type 4 (PTPN4) with the Mitogen-activated Protein Kinase p38 gamma. Maisonneuve, P., Caillet-Saguy, C., Vaney, M.C. et al. J Biol Chem (2016) 291:16699-16708. DOI 10.1074/jbc.M115.707208 · PubMed

Other PDB entries of the same protein (UniProt P29074 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3NFK 1.43 Å, Crystal structure of the PTPN4 PDZ domain complexed with the C-terminus of a rabies…
2VPH 1.9 Å, Crystal structure of the human protein tyrosine phosphatase, non- receptor type 4, PDZ…
3NFL 1.91 Å, Crystal structure of the PTPN4 PDZ domain complexed with the C-terminus of the GluN2A…
5EYZ 2.09 Å, Crystal structure of the PTPN4 PDZ domain complexed with the tailored peptide CYTO8-retev
2I75 2.45 Å, Crystal Structure of Human Protein Tyrosine Phosphatase N4 (PTPN4)
7VZE 2.88 Å, Crystal structure of PTPN4 PDZ bound to the PBM of HPV16 E6
2CS5 Solution structure of PDZ domain of Protein tyrosine phosphatase, non-receptor type 4

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