5F19: Aspirin Acetylated Human Cyclooxygenase-2

The Crystal Structure of Aspirin Acetylated Human Cyclooxygenase-2. Determined by X-ray diffraction at 2.04 Å resolution. Released 16 Mar 2016.

Method: X-ray diffraction
Resolution: 2.04 Å
Organism: Homo sapiens
Chains: 2
Atoms: 9,982
Mol. weight: 132.21 kDa
Ligands: COH, NAG, AKR, BOG
Released: 16 Mar 2016

Explore 5F19 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5F19 contains 89 α-helices and 66 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 44 helices, 34 β-strands

Element	Residues	Length	Sheet
β-strand	46-50	5	1
β-strand	54-58	5	1
β-strand	64-65	2	2
β-strand	71-72	2	2
α-helix	74-81	8
α-helix	86-93	8
α-helix	97-104	8
α-helix	106-121	16
β-strand	130	1	3
β-strand	131	1	4
β-strand	134	1	4
α-helix	139-143	5
β-strand	147	1	5
β-strand	149	1	6
β-strand	150	1	3
α-helix	153-156	4
β-strand	161	1	7
β-strand	164	1	7
α-helix	171-173	3
α-helix	174-177	4
α-helix	178-182	5
β-strand	183	1	8
β-strand	189	1	9
β-strand	194	1	10
β-strand	195	1	11
α-helix	196-206	11
β-strand	212	1	12
β-strand	220	1	5
β-strand	221	1	12
α-helix	231-234	4
α-helix	238-244	7
β-strand	245	1	13
α-helix	251	1
β-strand	252	1	13
α-helix	253	1
β-strand	255-257	3	14
β-strand	260-262	3	14
α-helix	263-264	2
β-strand	265	1	15
α-helix	266-269	4
α-helix	281-283	3
β-strand	285	1	15
α-helix	296-319	24
α-helix	325-343	19
α-helix	344-349	6
α-helix	350-353	4
α-helix	363-366	4
β-strand	378	1	6
α-helix	379-384	6
α-helix	388-390	3
β-strand	395-397	3	16
β-strand	400-402	3	16
α-helix	404-407	4
α-helix	412-428	17
β-strand	430	1	11
α-helix	431	1
β-strand	432	1	9
α-helix	433	1
β-strand	440	1	8
α-helix	442-444	3
α-helix	445-457	13
α-helix	460-462	3
α-helix	463-469	7
α-helix	472-475	4
α-helix	478-482	5
α-helix	486-495	10
α-helix	498-500	3
α-helix	503-509	7
α-helix	511	1
β-strand	512	1	17
α-helix	513	1
β-strand	519	1	17
α-helix	520-535	16
α-helix	538-540	3
α-helix	547-550	4
α-helix	553-560	8
α-helix	564-571	8
β-strand	581	1	10

Chain B: 45 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	35-38	4
β-strand	46-49	4	18
β-strand	55-58	4	18
β-strand	64-65	2	19
β-strand	71-72	2	19
α-helix	74-82	9
α-helix	83-85	3
α-helix	86-93	8
α-helix	97-104	8
α-helix	106-121	16
β-strand	130-131	2	20
β-strand	134	1	20
α-helix	139-143	5
β-strand	147	1	21
β-strand	149-150	2	20
α-helix	153-156	4
β-strand	161	1	22
β-strand	164	1	22
α-helix	171-173	3
α-helix	174-177	4
α-helix	178-182	5
β-strand	183	1	23
β-strand	189	1	24
β-strand	194	1	25
β-strand	195	1	26
α-helix	196-206	11
β-strand	212	1	27
β-strand	220	1	21
β-strand	221	1	27
α-helix	231-234	4
α-helix	238-244	7
β-strand	245	1	28
α-helix	251	1
β-strand	252	1	28
α-helix	253	1
β-strand	255-257	3	29
β-strand	260-262	3	29
β-strand	265	1	30
α-helix	266-269	4
α-helix	281-283	3
β-strand	285	1	30
α-helix	292-294	3
α-helix	296-319	24
α-helix	325-343	19
α-helix	344-349	6
α-helix	350-353	4
α-helix	363-366	4
β-strand	378	1	20
α-helix	379-384	6
α-helix	388-390	3
β-strand	395-397	3	31
β-strand	400-402	3	31
α-helix	404-407	4
α-helix	411-428	18
β-strand	430	1	26
α-helix	431	1
β-strand	432	1	24
α-helix	433	1
β-strand	440	1	23
α-helix	442-444	3
α-helix	445-457	13
α-helix	463-469	7
α-helix	473-475	3
α-helix	478-482	5
α-helix	486-495	10
α-helix	498-500	3
α-helix	503-509	7
α-helix	511	1
β-strand	512	1	32
α-helix	513	1
β-strand	519	1	32
α-helix	520-535	16
α-helix	538-540	3
α-helix	547-550	4
α-helix	553-560	8
α-helix	564-571	8
β-strand	581	1	25

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Prostaglandin G/H synthase 2	A, B	protein	552	Homo sapiens	P35354 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>5F19_1 Prostaglandin G/H synthase 2 (chains A, B)
KNPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCSTPEFLTRIKLFLKPTPNTVHYI
LTHFKGFWNVVNNIPFLRNAIMSYVLTSRSHLIDSPPTYNADYGYKSWEAFSNLSYYTRA
LPPVPDDCPTPLGVKGKKQLPDSNEIVEKLLLRRKFIPDPQGSNMMFAFFAQHFTHQFFK
TDHKRGPAFTNGLGHGVDLNHIYGETLARQRKLRLFKDGKMKYQIIDGEMYPPTVKDTQA
EMIYPPQVPEHLRFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQT
SRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNKQFQYQNRIAAEFNTLYHWHPLL
PDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQAS
IDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEK
PRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNN
VKGCPFTSFSVP

Ligands and cofactors

ID	Name	Formula	Copies
COH	Protoporphyrin IX containing co	C34 H32 Co N4 O4	2
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	3
AKR	Acrylic acid	C3 H4 O2	4
BOG	octyl beta-D-glucopyranoside	C14 H28 O6	1

Water and common crystallization additives (EDO) are not listed.

Primary citation

Crystal Structure of Aspirin-Acetylated Human Cyclooxygenase-2: Insight into the Formation of Products with Reversed Stereochemistry. Lucido, M.J., Orlando, B.J., Vecchio, A.J. et al. Biochemistry (2016) 55:1226-1238. DOI 10.1021/acs.biochem.5b01378 · PubMed

Other PDB entries of the same protein (UniProt P35354 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5IKR 2.34 Å, The Structure of Mefenamic Acid Bound to Human Cyclooxygenase-2
5F1A 2.38 Å, The Crystal Structure of Salicylate Bound to Human Cyclooxygenase-2
5IKQ 2.41 Å, The Structure of Meclofenamic Acid Bound to Human Cyclooxygenase-2
5IKT 2.45 Å, The Structure of Tolfenamic Acid Bound to Human Cyclooxygenase-2
5IKV 2.51 Å, The Structure of Flufenamic Acid Bound to Human Cyclooxygenase-2
5KIR 2.7 Å, The Structure of Vioxx Bound to Human COX-2

Browse structure collections

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