5T46: Human eIF4E-eIF4G complex

Name: PDB 5T46: Crystal structure of the human eIF4E-eIF4G complex
Published: 2016-10-26
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: translation

Crystal structure of the human eIF4E-eIF4G complex. Determined by X-ray diffraction at 1.53 Å resolution. Released 26 Oct 2016.

Method: X-ray diffraction
Resolution: 1.53 Å
Organism: Homo sapiens
Chains: 4
Atoms: 4,028
Mol. weight: 68.48 kDa
Ligands: MGP
Released: 26 Oct 2016

Explore 5T46 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5T46 contains 27 α-helices and 18 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	38-48	11	1
α-helix	56-59	4
β-strand	60-68	9	1
α-helix	69-76	8
β-strand	79	1	2
α-helix	82-84	3
α-helix	86	1
β-strand	90-95	6	1
β-strand	111-116	6	1
α-helix	119-121	3
α-helix	122-126	5
α-helix	127-138	12
α-helix	143-148	6
β-strand	149-155	7	1
β-strand	162-167	6	1
α-helix	173-187	15
β-strand	196-199	4	1
α-helix	200-205	6

Chains B and D: 3 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	614-618	5
α-helix	624-627	4
α-helix	629-630	2
β-strand	640	1	2

Chain C: 11 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	31-33	3
β-strand	38-48	11	3
α-helix	56-59	4
β-strand	60-68	9	3
α-helix	69-76	8
β-strand	79	1	4
α-helix	82-84	3
α-helix	86	1
β-strand	90-95	6	3
β-strand	111-116	6	3
α-helix	119-121	3
α-helix	122-126	5
α-helix	127-138	12
α-helix	143-148	6
β-strand	149-155	7	3
β-strand	162-167	6	3
α-helix	173-187	15
β-strand	196-199	4	3
α-helix	200-204	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Eukaryotic translation initiation factor 4E	A, C	protein	220	Homo sapiens	P06730 (AlphaFold model)
Eukaryotic translation initiation factor 4 gamma 1	B, D	protein	66	Homo sapiens	Q04637 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>5T46_1 Eukaryotic translation initiation factor 4E (chains A, C)
MLEMATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQ
ANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITL
NKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVT
HIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV

Sequence of entity 2 (B, D), FASTA

>5T46_2 Eukaryotic translation initiation factor 4 gamma 1 (chains B, D)
GPHMQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKT
PLRPLD

Ligands and cofactors

ID	Name	Formula	Copies
MGP	7-methyl-guanosine-5'-triphosphate	C11 H19 N5 O14 P3	2

Water and common crystallization additives (GOL) are not listed.

Primary citation

The Structures of eIF4E-eIF4G Complexes Reveal an Extended Interface to Regulate Translation Initiation. Gruner, S., Peter, D., Weber, R. et al. Mol Cell (2016) 64:467-479. DOI 10.1016/j.molcel.2016.09.020 · PubMed

Other PDB entries of the same protein (UniProt P06730 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4TPW 1.5 Å, The co-complex structure of the translation initiation factor eIF4E with the inhibitor…
8QM8 1.58 Å, Potential drug binding sites for translation initiation factor eIF4E
4TQB 1.59 Å, The co-complex structure of the translation initiation factor eIF4E with the inhibitor…
5ZJY 1.59 Å, Stapled-peptides tailored against initiation of translation
7D6Y 1.67 Å, eIF4E in Complex with a Disulphide-Free Autonomous VH Domain
5ZJZ 1.67 Å, Stapled-peptides tailored against initiation of translation
5EI3 1.71 Å, Co-crystal structure of eIF4E with nucleotide mimetic inhibitor.
4UED 1.75 Å, Complex of human eIF4E with the 4E binding protein 4E-BP1
5ZK9 1.76 Å, Stapled-peptides tailored against initiation of translation
4TQC 1.8 Å, The co-complex structure of the translation initiation factor eIF4E with the inhibitor…
5ZML 1.8 Å, Stapled-peptides tailored against initiation of translation
7XTP 1.83 Å, eIF4E in Complex with a Disulphide-Free Autonomous VH Domain

Browse structure collections

About this viewer

MolViewer shows 5T46 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.