Crystal structure of AacC2c1-sgRNA-extended target DNA ternary complex. Determined by X-ray diffraction at 2.92 Å resolution. Released 25 Jan 2017.
Explore 5U30 in 3D Show helices and sheets RCSB PDB PDBe
5U30 contains 47 α-helices and 38 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2-9 | 8 | 1 |
| β-strand | 10 | 1 | 2 |
| α-helix | 16-44 | 29 | |
| β-strand | 48-51 | 4 | 3 |
| α-helix | 52 | 1 | |
| β-strand | 59-63 | 5 | 3 |
| α-helix | 65-82 | 18 | |
| α-helix | 92-106 | 15 | |
| α-helix | 108-110 | 3 | |
| α-helix | 117-129 | 13 | |
| α-helix | 144-146 | 3 | |
| α-helix | 147-153 | 7 | |
| α-helix | 160-171 | 12 | |
| α-helix | 175-184 | 10 | |
| β-strand | 203 | 1 | 4 |
| α-helix | 204-208 | 5 | |
| α-helix | 215-256 | 42 | |
| α-helix | 261-276 | 16 | |
| α-helix | 282-283 | 2 | |
| α-helix | 299-307 | 9 | |
| α-helix | 315-328 | 14 | |
| α-helix | 336-342 | 7 | |
| α-helix | 349-352 | 4 | |
| α-helix | 357-373 | 17 | |
| β-strand | 393-394 | 2 | 1 |
| β-strand | 395 | 1 | 5 |
| β-strand | 403 | 1 | 5 |
| β-strand | 404-407 | 4 | 4 |
| β-strand | 417-427 | 11 | 4 |
| β-strand | 430-440 | 11 | 4 |
| β-strand | 441 | 1 | 2 |
| α-helix | 445-449 | 5 | |
| β-strand | 450-452 | 3 | 1 |
| β-strand | 460-463 | 4 | 1 |
| α-helix | 471 | 1 | |
| β-strand | 472-483 | 12 | 1 |
| β-strand | 500-508 | 9 | 1 |
| α-helix | 511-514 | 4 | |
| α-helix | 521-524 | 4 | |
| β-strand | 526-529 | 4 | 6 |
| β-strand | 534-537 | 4 | 6 |
| α-helix | 542-548 | 7 | |
| α-helix | 556-559 | 4 | |
| β-strand | 565-571 | 7 | 6 |
| β-strand | 577-586 | 10 | 6 |
| β-strand | 601-603 | 3 | 6 |
| β-strand | 610-620 | 11 | 6 |
| α-helix | 621-622 | 2 | |
| α-helix | 629-637 | 9 | |
| α-helix | 640-657 | 18 | |
| α-helix | 666-674 | 9 | |
| α-helix | 686-700 | 15 | |
| α-helix | 708-738 | 31 | |
| α-helix | 758-775 | 18 | |
| α-helix | 795-820 | 26 | |
| β-strand | 823-826 | 4 | 7 |
| β-strand | 834-837 | 4 | 7 |
| α-helix | 840-842 | 3 | |
| β-strand | 844-849 | 6 | 6 |
| α-helix | 861-870 | 10 | |
| α-helix | 872-883 | 12 | |
| β-strand | 889-894 | 6 | 6 |
| β-strand | 901 | 1 | 8 |
| β-strand | 908 | 1 | 8 |
| β-strand | 910-914 | 5 | 9 |
| α-helix | 917-919 | 3 | |
| α-helix | 925-928 | 4 | |
| α-helix | 930-939 | 10 | |
| β-strand | 951-953 | 3 | 9 |
| β-strand | 959-963 | 5 | 9 |
| β-strand | 971-975 | 5 | 9 |
| α-helix | 976-990 | 15 | |
| α-helix | 994-996 | 3 | |
| β-strand | 998-1005 | 8 | 10 |
| β-strand | 1010-1014 | 5 | 10 |
| α-helix | 1015 | 1 | |
| α-helix | 1018-1025 | 8 | |
| β-strand | 1029-1031 | 3 | 10 |
| β-strand | 1037-1041 | 5 | 10 |
| β-strand | 1075-1078 | 4 | 10 |
| α-helix | 1086-1088 | 3 | |
| β-strand | 1090-1092 | 3 | 10 |
| α-helix | 1093-1101 | 9 | |
| α-helix | 1102-1106 | 5 | |
| α-helix | 1107-1112 | 6 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| CRISPR-associated endonuclease C2c1 | A | protein | 1130 | Alicyclobacillus acidoterrestris | T0D7A2 (AlphaFold model) |
| sgRNA | B | RNA | 112 | Alicyclobacillus acidoterrestris | |
| Target DNA strand | C | DNA | 38 | Homo sapiens | |
| Non-target DNA strand | D | DNA | 8 | Homo sapiens |
>5U30_1 CRISPR-associated endonuclease C2c1 (chains A) SMAVKSIKVKLRLDDMPEIRAGLWKLHKEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQ ECDKTAEECKAELLERLRARQVENGHRGPAGSDDELLQLARQLYELLVPQAIGAKGDAQQ IARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKEKAETRKSADRTADVL RALADFGLKPLMRVYTDSEMSSVEWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVG QEYAKLVEQKNRFEQKNFVGQEHLVHLVNQLQQDMKEASPGLESKEQTAHYVTGRALRGS DKVFEKWGKLAPDAPFDLYDAEIKNVQRRNTRRFGSHDLFAKLAEPEYQALWREDASFLT RYAVYNSILRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGERRHAIR FHKLLKVENGVAREVDDVTVPISMSEQLDNLLPRDPNEPIALYFRDYGAEQHFTGEFGGA KIQCRRDQLAHMHRRRGARDVYLNVSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHF DKLSDYLAEHPDDGKLGSEGLLSGLRVMSVALGLRTSASISVFRVARKDELKPNSKGRVP FFFPIKGNDNLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCG SEDVGRRERSWAKLIEQPVDAANHMTPDWREAFENELQKLKSLHGICSDKEWMDAVYESV RRVWRHMGKQVRDWRKDVRSGERPKIRGYAKDVVGGNSIEQIEYLERQYKFLKSWSFFGK VSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDERGKGKWVAKY PPCQLILLAELSEYQFNNDRPPSENNQLMQWSHRGVFQELINQAQVHDLLVGTMYAAFSS RFDARTGAPGIRCRRVPARCTQEHNPEPFPWWLNKFVVEHTLDACPLRADDLIPTGEGEI FVSPFSAEEGDFHQIHAALNAAQNLQQRLWSDFDISQIRLRCDWGEVDGELVLIPRLTGK RTADSYSNKVFYTNTGVTYYERERGKKRRKVFAQEKLSEEEAELLVEADEAREKSVVLMR DPSGIINRGNWTRQKEFWSMVNQRIEGYLVKQIRSRVPLQDSACENTGDI
>5U30_2 sgRNA (chains B) GGUCUAGAGGACAGAAUUUUUCAACGGGUGUGCCAAUGGCCACUUUCCAGGUGGCAAAGC CCGUUGAGCUUCUCAAAUCUGAGAAGUGGCACCAGAACCGGAGGACAAAGUC
>5U30_3 Target DNA strand (chains C) CTGCCGTTTGGACTTTGTCCTCCGGTTCTGGAACCACA
>5U30_4 Non-target DNA strand (chains D) TGTGGTTC
PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease. Yang, H., Gao, P., Rajashankar, K.R. et al. Cell (2016) 167:1814-1828.e12. DOI 10.1016/j.cell.2016.11.053 · PubMed
Other PDB entries of the same protein (UniProt T0D7A2 (AlphaFold model), which also has an AlphaFold model), best resolution first:
5U30 is part of these collections:
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