CRISPR and genome editing

CRISPR systems began as a bacterial immune defense against viruses and became a precise tool for editing genes, recognized by the 2020 Nobel Prize in Chemistry. Cas9 uses a guide RNA to find a matching DNA sequence next to a short PAM motif and then cuts both strands. The collection also covers Cas12, Cas13, base editors and older programmable tools such as TALEs and zinc fingers.

Open Cas9 with guide RNA and target DNA in 3D

19 structures

Cas9 with guide RNA and target DNA4OO8
Cas9 without RNA (apo)4CMP
Cas9 with single-guide RNA4ZT0
Cas9 bound to a PAM-containing target4UN3
Cas9 R-loop complex primed for DNA cleavage5F9R
Cas9-sgRNA-DNA complex by cryo-EM5Y36
Cas9 ternary complex with magnesium6O0Y
Staphylococcus aureus Cas9 with sgRNA and DNA5AXW
SpRY, a near-PAMless Cas9 variant8SRS
ABE8e adenine base editor6VPC
A base editor changes a single DNA letter without cutting both strands.
Cas12a (Cpf1) with crRNA and target DNA5B43
FnCas12a with crRNA and DNA6I1K
Cas12b (C2c1) with sgRNA and target DNA5U30
Cas13a with CRISPR RNA5W1H
Cas13 targets RNA instead of DNA and is used in diagnostic tests.
Cas13a with crRNA and target RNA5XWP
Cascade surveillance complex bound to DNA4QYZ
Cas1-Cas2 integrase with DNA5DQZ
TAL effector PthXo1 bound to DNA3UGM
Zif268 zinc fingers bound to DNA1ZAA

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