Crystal structure of AacC2c1-sgRNA binary complex. Determined by X-ray diffraction at 3.25 Å resolution. Released 25 Jan 2017.
Explore 5U34 in 3D Show helices and sheets RCSB PDB PDBe
5U34 contains 41 α-helices and 38 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2-8 | 7 | 1 |
| β-strand | 10 | 1 | 2 |
| α-helix | 16-44 | 29 | |
| α-helix | 68-80 | 13 | |
| α-helix | 92-106 | 15 | |
| α-helix | 108-110 | 3 | |
| α-helix | 118-128 | 11 | |
| α-helix | 175-184 | 10 | |
| β-strand | 203 | 1 | 3 |
| α-helix | 205-208 | 4 | |
| α-helix | 215-256 | 42 | |
| α-helix | 261-276 | 16 | |
| α-helix | 294-296 | 3 | |
| α-helix | 299-306 | 8 | |
| α-helix | 315-325 | 11 | |
| α-helix | 336-342 | 7 | |
| α-helix | 346-348 | 3 | |
| α-helix | 349-352 | 4 | |
| α-helix | 357-372 | 16 | |
| α-helix | 378-380 | 3 | |
| α-helix | 391 | 1 | |
| β-strand | 392-394 | 3 | 1 |
| β-strand | 395 | 1 | 4 |
| β-strand | 403 | 1 | 4 |
| β-strand | 404-407 | 4 | 5 |
| β-strand | 417-420 | 4 | 5 |
| β-strand | 423-426 | 4 | 3 |
| β-strand | 431-434 | 4 | 3 |
| β-strand | 437-440 | 4 | 5 |
| β-strand | 441 | 1 | 2 |
| α-helix | 447-449 | 3 | |
| β-strand | 450-452 | 3 | 1 |
| β-strand | 460-465 | 6 | 1 |
| β-strand | 468-481 | 14 | 1 |
| β-strand | 502-508 | 7 | 1 |
| α-helix | 509-510 | 2 | |
| α-helix | 511-514 | 4 | |
| α-helix | 521-524 | 4 | |
| β-strand | 526-529 | 4 | 6 |
| β-strand | 534-537 | 4 | 6 |
| α-helix | 542-548 | 7 | |
| α-helix | 556-561 | 6 | |
| β-strand | 565-570 | 6 | 6 |
| β-strand | 577-586 | 10 | 6 |
| β-strand | 601-602 | 2 | 6 |
| β-strand | 610-620 | 11 | 6 |
| α-helix | 629-655 | 27 | |
| α-helix | 657-659 | 3 | |
| α-helix | 667-673 | 7 | |
| α-helix | 686-700 | 15 | |
| α-helix | 714-721 | 8 | |
| α-helix | 723-738 | 16 | |
| α-helix | 758-775 | 18 | |
| α-helix | 795-820 | 26 | |
| β-strand | 823-826 | 4 | 7 |
| β-strand | 834-837 | 4 | 7 |
| α-helix | 840-842 | 3 | |
| β-strand | 844-848 | 5 | 6 |
| α-helix | 861-864 | 4 | |
| α-helix | 866-882 | 17 | |
| β-strand | 889-893 | 5 | 6 |
| β-strand | 901 | 1 | 8 |
| β-strand | 908 | 1 | 8 |
| β-strand | 911-913 | 3 | 9 |
| α-helix | 932-939 | 8 | |
| β-strand | 952-953 | 2 | 9 |
| β-strand | 959-963 | 5 | 9 |
| β-strand | 971-975 | 5 | 9 |
| α-helix | 976-989 | 14 | |
| α-helix | 994-996 | 3 | |
| β-strand | 999-1003 | 5 | 10 |
| β-strand | 1012-1014 | 3 | 10 |
| α-helix | 1019-1024 | 6 | |
| β-strand | 1029-1032 | 4 | 10 |
| β-strand | 1037-1041 | 5 | 10 |
| β-strand | 1076-1078 | 3 | 10 |
| α-helix | 1085-1088 | 4 | |
| β-strand | 1090-1092 | 3 | 10 |
| α-helix | 1093-1113 | 21 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| CRISPR-associated endonuclease C2c1 | A | protein | 1130 | Alicyclobacillus acidoterrestris | T0D7A2 (AlphaFold model) |
| sgRNA | B | RNA | 112 | Alicyclobacillus acidoterrestris |
>5U34_1 CRISPR-associated endonuclease C2c1 (chains A) SMAVKSIKVKLRLDDMPEIRAGLWKLHKEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQ ECDKTAEECKAELLERLRARQVENGHRGPAGSDDELLQLARQLYELLVPQAIGAKGDAQQ IARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKEKAETRKSADRTADVL RALADFGLKPLMRVYTDSEMSSVEWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVG QEYAKLVEQKNRFEQKNFVGQEHLVHLVNQLQQDMKEASPGLESKEQTAHYVTGRALRGS DKVFEKWGKLAPDAPFDLYDAEIKNVQRRNTRRFGSHDLFAKLAEPEYQALWREDASFLT RYAVYNSILRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGERRHAIR FHKLLKVENGVAREVDDVTVPISMSEQLDNLLPRDPNEPIALYFRDYGAEQHFTGEFGGA KIQCRRDQLAHMHRRRGARDVYLNVSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHF DKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSKGRVP FFFPIKGNDNLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCG SEDVGRRERSWAKLIEQPVDAANHMTPDWREAFENELQKLKSLHGICSDKEWMDAVYESV RRVWRHMGKQVRDWRKDVRSGERPKIRGYAKDVVGGNSIEQIEYLERQYKFLKSWSFFGK VSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDERGKGKWVAKY PPCQLILLAELSEYQFNNDRPPSENNQLMQWSHRGVFQELINQAQVHDLLVGTMYAAFSS RFDARTGAPGIRCRRVPARCTQEHNPEPFPWWLNKFVVEHTLDACPLRADDLIPTGEGEI FVSPFSAEEGDFHQIHADLNAAQNLQQRLWSDFDISQIRLRCDWGEVDGELVLIPRLTGK RTADSYSNKVFYTNTGVTYYERERGKKRRKVFAQEKLSEEEAELLVEADEAREKSVVLMR DPSGIINRGNWTRQKEFWSMVNQRIEGYLVKQIRSRVPLQDSACENTGDI
>5U34_2 sgRNA (chains B) GGUCUAGAGGACAGAAUUUUUCAACGGGUGUGCCAAUGGCCACUUUCCAGGUGGCAAAGC CCGUUGAGCUUCUCAAAUCUGAGAAGUGGCACCAGAACCGGAGGACAAAGUC
PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease. Yang, H., Gao, P., Rajashankar, K.R. et al. Cell (2016) 167:1814-1828.e12. DOI 10.1016/j.cell.2016.11.053 · PubMed
Other PDB entries of the same protein (UniProt T0D7A2 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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