5VMS: Xenopus KCNQ1 channel

CryoEM structure of Xenopus KCNQ1 channel. Determined by electron microscopy at 3.7 Å resolution. Released 7 Jun 2017.

Method: Electron microscopy
Resolution: 3.7 Å
Organisms: Xenopus laevis, Homo sapiens
Chains: 2
Atoms: 3,729
Mol. weight: 79.64 kDa
Ligands: CA
Released: 7 Jun 2017

Explore 5VMS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5VMS contains 29 α-helices and 6 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 20 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	96-104	9
α-helix	110-134	25
α-helix	136-138	3
α-helix	140-167	28
α-helix	168-170	3
α-helix	176-185	10
α-helix	187-205	19
α-helix	219-227	9
α-helix	228-231	4
α-helix	233-235	3
α-helix	236-247	12
α-helix	249-273	25
β-strand	278	1	1
β-strand	284	1	1
α-helix	289-301	13
α-helix	313-349	37
α-helix	351-353	3
α-helix	354-356	3
α-helix	357-375	19
α-helix	498-522	25
α-helix	524-525	2
α-helix	527-555	29

Chain B: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	7-21	15
β-strand	28	1	2
α-helix	30-40	11
α-helix	46-56	11
β-strand	64	1	2
α-helix	66-74	9
α-helix	80-93	14
α-helix	95-97	3
β-strand	101	1	3
α-helix	103-112	10
α-helix	119-129	11
β-strand	137	1	3
α-helix	139-145	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Potassium voltage-gated channel subfamily KQT member 1	A	protein	548	Xenopus laevis	P70057 (AlphaFold model)
Calmodulin	B	protein	149	Homo sapiens	P0DP23 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5VMS_1 Potassium voltage-gated channel subfamily KQT member 1 (chains A)
MATDPPRPTINLDPRVSIYSGRRPLLSRTNIQGRVYNFLERPTGWKCFVYHFTVFLIVLI
CLIFSVLSTIQQYNNLATETLFWMEIVLVVFFGAEYVVRLWSAGCRSKYVGVWGRLRFAR
KPISVIDLIVVVASVIVLCVGSNGQVFATSAIRGIRFLQILRMLHVDRQGGTWRLLGSVV
FIHRQELITTLYIGFLGLIFSSYFVYLAEKDAIDSSGEYQFGSYADALWWGVVTVTTIGY
GDKVPQTWIGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAASLI
QTAWRCYAAENPDSATWKIYIRKQSRNHHLMSPSPKPKKSAMVKKKKIRTERDEGSTDKM
LNIPHITYDHVADDRKNDGYSVESYENTVRKPFGFLDPSTGPFIRTSSFTDDLDMEGDTL
LTPITHISELKEHHRAAIKVIRRMQYFVAKKKFQQARKPYDVRDVIEQYSQGHLNLMVRI
KELQRRLDQSLGKPSLFLSVSDKVKDKGINTIGSRLNRVEDKVTQMDHKLNLITDMLHHL
LTNQQSNS

Sequence of entity 2 (B), FASTA

>5VMS_2 Calmodulin (chains B)
MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE
EVDEMIREADIDGDGQVNYEEFVQMMTAK

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	3

Primary citation

Cryo-EM Structure of a KCNQ1/CaM Complex Reveals Insights into Congenital Long QT Syndrome. Sun, J., MacKinnon, R. Cell (2017) 169:1042-1050.e9. DOI 10.1016/j.cell.2017.05.019 · PubMed

Other PDB entries of the same protein (UniProt P70057 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9MY3 3.46 Å, Structure of Xenopus KCNQ1-CaM in GDN
9MY4 3.53 Å, Structure of Xenopus KCNQ1(E150R/R221E)-CaM with the VSD in the intermediate state
10NL 3.81 Å, Xenopus KCNQ1 in complex with UCL2077
7TCP 3.84 Å, Structure of Xenopus KCNQ1-CaM
7TCI 3.9 Å, Structure of Xenopus KCNQ1-CaM in complex with ML277
10NK 3.95 Å, Xenopus KCNQ1 Intermediate State (E1R/R2E) in complex with UCL2077

Browse structure collections

About this viewer

MolViewer shows 5VMS directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.