5VOB: HCMV Pentamer
Crystal structure of HCMV Pentamer in complex with neutralizing antibody 8I21. Determined by X-ray diffraction at 3.02 Å resolution. Released 5 Jul 2017.
- Method
- X-ray diffraction
- Resolution
- 3.02 Å
- Organisms
- Human cytomegalovirus, Human cytomegalovirus (strain 5508), Human cytomegalovirus (strain AD169)
- Chains
- 7
- Atoms
- 14,099
- Mol. weight
- 236.1 kDa
- Ligands
- NAG
- Released
- 5 Jul 2017
Explore 5VOB in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
5VOB contains 82 α-helices and 104 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 40 helices, 34 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 49-51 | 3 | 1 |
| β-strand | 64-66 | 3 | 2 |
| β-strand | 69-71 | 3 | 1 |
| β-strand | 76-83 | 8 | 2 |
| β-strand | 86-92 | 7 | 2 |
| α-helix | 94-97 | 4 | |
| α-helix | 100-106 | 7 | |
| α-helix | 115-122 | 8 | |
| β-strand | 125 | 1 | 3 |
| α-helix | 126-127 | 2 | |
| α-helix | 128-131 | 4 | |
| α-helix | 134-135 | 2 | |
| β-strand | 136-137 | 2 | 4 |
| α-helix | 156-158 | 3 | |
| α-helix | 164 | 1 | |
| β-strand | 165 | 1 | 5 |
| α-helix | 166-167 | 2 | |
| β-strand | 182-183 | 2 | 6 |
| α-helix | 189-191 | 3 | |
| β-strand | 193-197 | 5 | 4 |
| β-strand | 204-205 | 2 | 7 |
| β-strand | 207-217 | 11 | 4 |
| β-strand | 224-230 | 7 | 4 |
| β-strand | 233-239 | 7 | 4 |
| β-strand | 246-251 | 6 | 4 |
| β-strand | 257-258 | 2 | 7 |
| α-helix | 259 | 1 | |
| α-helix | 264-267 | 4 | |
| β-strand | 268-271 | 4 | 4 |
| β-strand | 276-280 | 5 | 4 |
| β-strand | 282 | 1 | 3 |
| α-helix | 285-287 | 3 | |
| α-helix | 290-292 | 3 | |
| α-helix | 299-302 | 4 | |
| α-helix | 309-315 | 7 | |
| α-helix | 317-327 | 11 | |
| α-helix | 335-352 | 18 | |
| α-helix | 354-358 | 5 | |
| β-strand | 361-362 | 2 | 6 |
| α-helix | 363-384 | 22 | |
| α-helix | 388-390 | 3 | |
| α-helix | 397-408 | 12 | |
| β-strand | 415 | 1 | 5 |
| α-helix | 416-428 | 13 | |
| α-helix | 432-434 | 3 | |
| α-helix | 437-453 | 17 | |
| α-helix | 458-460 | 3 | |
| α-helix | 461-475 | 15 | |
| α-helix | 481-493 | 13 | |
| α-helix | 497-505 | 9 | |
| α-helix | 515-518 | 4 | |
| α-helix | 521-523 | 3 | |
| β-strand | 527-528 | 2 | 8 |
| α-helix | 532-536 | 5 | |
| α-helix | 548-557 | 10 | |
| β-strand | 578-581 | 4 | 8 |
| β-strand | 587-592 | 6 | 8 |
| β-strand | 597 | 1 | 9 |
| β-strand | 600-603 | 4 | 8 |
| β-strand | 614-619 | 6 | 8 |
| α-helix | 623-624 | 2 | |
| β-strand | 626 | 1 | 9 |
| β-strand | 633 | 1 | 8 |
| α-helix | 635-636 | 2 | |
| β-strand | 637-638 | 2 | 10 |
| α-helix | 639 | 1 | |
| α-helix | 645-647 | 3 | |
| β-strand | 652-658 | 7 | 10 |
| β-strand | 663-669 | 7 | 10 |
| α-helix | 672-679 | 8 | |
| α-helix | 682-684 | 3 | |
| β-strand | 692-697 | 6 | 10 |
| β-strand | 703-705 | 3 | 10 |
| α-helix | 707-710 | 4 | |
Chain B: 10 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 47-56 | 10 | |
| α-helix | 81 | 1 | |
| β-strand | 82-83 | 2 | 11 |
| α-helix | 84-86 | 3 | |
| α-helix | 103-112 | 10 | |
| α-helix | 118-126 | 9 | |
| α-helix | 133-142 | 10 | |
| β-strand | 150-154 | 5 | 12 |
| β-strand | 158-163 | 6 | 12 |
| β-strand | 169-170 | 2 | 11 |
| β-strand | 178-184 | 7 | 2 |
| β-strand | 188-197 | 10 | 2 |
| β-strand | 202-211 | 10 | 2 |
| α-helix | 216-219 | 4 | |
| α-helix | 220-235 | 16 | |
| α-helix | 241-252 | 12 | |
| α-helix | 259-261 | 3 | |
Chain C: 7 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 36 | 1 | 13 |
| α-helix | 39-40 | 2 | |
| β-strand | 45-48 | 4 | 13 |
| β-strand | 54-57 | 4 | 13 |
| β-strand | 62-65 | 4 | 13 |
| α-helix | 70-81 | 12 | |
| α-helix | 86-92 | 7 | |
| α-helix | 94-96 | 3 | |
| α-helix | 100 | 1 | |
| β-strand | 101-103 | 3 | 14 |
| β-strand | 109-111 | 3 | 14 |
| α-helix | 112-113 | 2 | |
| β-strand | 121-122 | 2 | 14 |
| β-strand | 125 | 1 | 15 |
| β-strand | 130-132 | 3 | 16 |
| α-helix | 146-154 | 9 | |
Chain D: 8 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 49-53 | 5 | |
| α-helix | 54-56 | 3 | |
| α-helix | 62-65 | 4 | |
| α-helix | 68-70 | 3 | |
| β-strand | 71-78 | 8 | 12 |
| α-helix | 81-83 | 3 | |
| β-strand | 87-93 | 7 | 12 |
| β-strand | 98-102 | 5 | 12 |
| α-helix | 105-117 | 13 | |
| α-helix | 120-130 | 11 | |
| α-helix | 141-150 | 10 | |
| β-strand | 154-165 | 12 | 16 |
| β-strand | 168-182 | 15 | 16 |
| β-strand | 188-198 | 11 | 16 |
| β-strand | 212 | 1 | 15 |
Chain E: 6 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 22-25 | 4 | |
| α-helix | 36-40 | 5 | |
| α-helix | 42-45 | 4 | |
| α-helix | 46-68 | 23 | |
| α-helix | 74-78 | 5 | |
| α-helix | 82-94 | 13 | |
| β-strand | 97-98 | 2 | 16 |
| β-strand | 107-113 | 7 | 16 |
| β-strand | 117-126 | 10 | 16 |
Chain H: 6 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 17 |
| β-strand | 11-12 | 2 | 18 |
| β-strand | 18-25 | 8 | 17 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 19 |
| β-strand | 45-51 | 7 | 19 |
| β-strand | 58-60 | 3 | 19 |
| α-helix | 62-64 | 3 | |
| β-strand | 68-73 | 6 | 17 |
| β-strand | 78-83 | 6 | 17 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-103 | 12 | 19 |
| β-strand | 106-112 | 7 | 19 |
| β-strand | 116-118 | 3 | 19 |
| β-strand | 119-120 | 2 | 18 |
| α-helix | 123-125 | 3 | |
| β-strand | 126 | 1 | 20 |
| β-strand | 129-133 | 5 | 21 |
| β-strand | 145-154 | 10 | 21 |
| β-strand | 155 | 1 | 20 |
| β-strand | 163 | 1 | 22 |
| β-strand | 172-174 | 3 | 21 |
| α-helix | 175-177 | 3 | |
| β-strand | 178-179 | 2 | 21 |
| β-strand | 185-193 | 9 | 21 |
| α-helix | 195-197 | 3 | |
| β-strand | 203-209 | 7 | 22 |
| β-strand | 214-220 | 7 | 22 |
Chain L: 5 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4-7 | 4 | 23 |
| β-strand | 10-13 | 4 | 24 |
| β-strand | 19-25 | 7 | 23 |
| β-strand | 33-38 | 6 | 24 |
| β-strand | 45-49 | 5 | 24 |
| β-strand | 53-54 | 2 | 24 |
| α-helix | 55 | 1 | |
| β-strand | 62-67 | 6 | 23 |
| β-strand | 70-75 | 6 | 23 |
| α-helix | 80-82 | 3 | |
| β-strand | 85-90 | 6 | 24 |
| β-strand | 98-99 | 2 | 24 |
| β-strand | 103-107 | 5 | 24 |
| β-strand | 112 | 1 | 25 |
| β-strand | 115-119 | 5 | 26 |
| α-helix | 120-122 | 3 | |
| β-strand | 130-140 | 11 | 26 |
| β-strand | 141 | 1 | 25 |
| β-strand | 145-151 | 7 | 27 |
| β-strand | 155 | 1 | 27 |
| β-strand | 160-164 | 5 | 26 |
| α-helix | 165-168 | 4 | |
| β-strand | 174-183 | 10 | 26 |
| α-helix | 184-189 | 6 | |
| β-strand | 192-199 | 8 | 27 |
| β-strand | 206-207 | 2 | 27 |
| β-strand | 210-211 | 2 | 27 |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Envelope glycoprotein H | A | protein | 725 | Human cytomegalovirus | Q6SW67 (AlphaFold model) |
| Envelope glycoprotein L | B | protein | 278 | Human cytomegalovirus (strain 5508) | Q68674 |
| Envelope glycoprotein UL128 | C | protein | 171 | Human cytomegalovirus (strain AD169) | P16837 |
| Envelope glycoprotein UL130 | D | protein | 252 | Human cytomegalovirus (strain Merlin) | F5HCP3 (AlphaFold model) |
| Envelope glycoprotein UL131A | E | protein | 129 | Human cytomegalovirus (strain Merlin) | F5HET4 |
| Fab 8I21 heavy chain | H | protein | 289 | Homo sapiens | S6B291 |
| Fab 8I21 light chain | L | protein | 235 | Homo sapiens | Q6GMX0 |
Sequence of entity 1 (A), FASTA
>5VOB_1 Envelope glycoprotein H (chains A)
MRPGLPSYLIILAVCLFSHLLSSRYGAEAVSEPLDKAFHLLLNTYGRPIRFLRENTTQCT
YNSSLRNSTVVRENAISFNFFQSYNQYYVFHMPRCLFAGPLAEQFLNQVDLTETLERYQQ
RLNTYALVSKDLASYRSFSQQLKAQDSLGEQPTTVPPPIDLSIPHVWMPPQTTPHGWTES
HTTSGLHRPHFNQTCILFDGHDLLFSTVTPCLHQGFYLIDELRYVKITLTEDFFVVTVSI
DDDTPMLLIFGHLPRVLFKAPYQRDNFILRQTEKHELLVLVKKDQLNRHSYLKDPDFLDA
ALDFNYLDLSALLRNSFHRYAVDVLKSGRCQMLDRRTVEMAFAYALALFAAARQEEAGAQ
VSVPRALDRQAALLQIQEFMITCLSQTPPRTTLLLYPTAVDLAKRALWTPNQITDITSLV
RLVYILSKQNQQHLIPQWALRQIADFALKLHKTHLASFLSAFARQELYLMGSLVHSMLVH
TTERREIFIVETGLCSLAELSHFTQLLAHPHHEYLSDLYTPCSSSGRRDHSLERLTRLFP
DATVPATVPAALSILSTMQPSTLETFPDLFCLPLGESFSALTVSEHVSYIVTNQYLIKGI
SYPVSTTVVGQSLIITQTDSQTKCELTRNMHTTHSITVALNISLENCAFCQSALLEYDDT
QGVINIMYMHDSDDVLFALDPYNEVVVSSPRTHYLMLLKNGTVLEVTDVVVDATDGSGSH
HHHHH
Sequence of entity 2 (B), FASTA
>5VOB_2 Envelope glycoprotein L (chains B)
MCRRPDCGFSFSPGPVILLWCCLLLPIVSSAAVSVAPTAAEKVPAECPELTRRCLLGEVF
EGDKYESWLRPLVNVTGRDGPLSQLIRYRPVTPEAANSVLLDEAFLDTLALLYNNPDQLR
ALLTLLSSDTAPRWMTVMRGYSECGDGSPAVYTCVDDLCRGYDLTRLSYGRSIFTEHVLG
FELVPPSLFNVVVAIRNEATRTNRAVRLPVSTAAAPEGITLFYGLYNAVKEFCLRHQLDP
PLLRHLDKYYAGLPPELKQTRVNLPAHSRYGPQAVDAR
Sequence of entity 3 (C), FASTA
>5VOB_3 Envelope glycoprotein UL128 (chains C)
MSPKDLTPFLTTLWLLLGHSRVPRVRAEECCEFINVNHPPERCYDFKMCNRFTVALRCPD
GEVCYSPEKTAEIRGIVTTMTHSLTRQVVHNKLTSCNYNPLYLEADGRIRCGKVNDKAQY
LLGAAGSVPYRWINLEYDKITRIVGLDQYLESVKKHKRLDVCRAKMGYMLQ
Sequence of entity 4 (D), FASTA
>5VOB_4 Envelope glycoprotein UL130 (chains D)
MLRLLLRHHFHCLLLCAVWATPCLASPWSTLTANQNPSPPWSKLTYSKPHDAATFYCPFL
YPSPPRSPLQFSGFQRVSTGPECRNETLYLLYNREGQTLVERSSTWVKKVIWYLSGRNQT
ILQRMPRTASKPSDGNVQISVEDAKIFGAHMVPKQTKLLRFVVNDGTRYQMCVMKLESWA
HVFRDYSVSFQVRLTFTEANNQTYTFCTHPNLIVGSENLYFQAGWSHPQFEKGGGSGGGS
GGGSWSHPQFEK
Sequence of entity 5 (E), FASTA
>5VOB_5 Envelope glycoprotein UL131A (chains E)
MRLCRVWLSVCLCAVVLGQCQRETAEKNDYYRVPHYWDACSRALPDQTRYKYVEQLVDLT
LNYHYDASHGLDNFDVLKRINVTEVSLLISDFRRQNRRGGTNKRTTFNAAGSLAPHARSL
EFSVRLFAN
Sequence of entity 6 (H), FASTA
>5VOB_6 Fab 8I21 heavy chain (chains H)
MEFGLSWVFLVAILEGVHCLVELVESGGGVVQPGRSLRLSCAASGFTFSSDGMHWVRQSP
GRGLEWVAFISSDGSTPYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAMYFCAKDWA
LFRWLRTFDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTV
SWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVE
PKSCDKSSGLEVLFQGPLGSAWSHPQFEKGGGSGGGSGGGSWSHPQFEK
Sequence of entity 7 (L), FASTA
>5VOB_7 Fab 8I21 light chain (chains L)
METPAELLFLLLLWLPDTTGETVMTQSPATLSVSPGGRATLSCRASQSVGINLAWYQQKP
GQAPRLLIYGASTRASGFPARFSGSGSGTEFTLTITSLQSEDFAVYYCQQYNDWPPWTFG
QGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNS
QESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 10 |
Primary citation
Structural basis for potent antibody-mediated neutralization of human cytomegalovirus. Chandramouli, S., Malito, E., Nguyen, T. et al. Sci Immunol (2017) 2. DOI 10.1126/sciimmunol.aan1457 · PubMed
Other PDB entries of the same protein (UniProt Q6SW67 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7LBG 2.6 Å, CryoEM structure of the HCMV Trimer gHgLgO in complex with human Transforming growth…
- 7LBF 2.8 Å, CryoEM structure of the HCMV Trimer gHgLgO in complex with human Platelet-derived growth…
- 7LBE 2.9 Å, CryoEM structure of the HCMV Trimer gHgLgO in complex with neutralizing fabs 13H11 and…
- 7T4Q 2.9 Å, CryoEM structure of the HCMV Pentamer gH/gL/UL128/UL130/UL131A in complex with…
- 7T4S 3.1 Å, CryoEM structure of the HCMV Pentamer gH/gL/UL128/UL130/UL131A in complex with NRP2 and…
- 7T4R 3.3 Å, CryoEM structure of the HCMV Pentamer gH/gL/UL128/UL130/UL131A in complex with THBD and…
- 5VOC 3.99 Å, Crystal structure of HCMV Pentamer in complex with neutralizing antibody 8I21 - Low…
- 5VOD 5.9 Å, Crystal structure of HCMV Pentamer in complex with neutralizing antibody 9I6
Browse structure collections
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