Crystal structure of Alicyclobacillus acidoterrestris C2c1 in complex with single-guide RNA at 3.1 Angstrom resolution. Determined by X-ray diffraction at 3.13 Å resolution. Released 25 Jan 2017.
Explore 5WQE in 3D Show helices and sheets RCSB PDB PDBe
5WQE contains 37 α-helices and 39 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 3-9 | 7 | 1 |
| β-strand | 10 | 1 | 2 |
| α-helix | 16-43 | 28 | |
| β-strand | 49-51 | 3 | 3 |
| β-strand | 60-63 | 4 | 3 |
| α-helix | 65-82 | 18 | |
| α-helix | 92-106 | 15 | |
| α-helix | 108-111 | 4 | |
| α-helix | 117-129 | 13 | |
| α-helix | 176-184 | 9 | |
| β-strand | 203 | 1 | 4 |
| α-helix | 206-207 | 2 | |
| α-helix | 215-256 | 42 | |
| α-helix | 261-274 | 14 | |
| α-helix | 299-306 | 8 | |
| α-helix | 315-329 | 15 | |
| α-helix | 336-342 | 7 | |
| α-helix | 348-351 | 4 | |
| α-helix | 354 | 1 | |
| α-helix | 357-372 | 16 | |
| β-strand | 392-393 | 2 | 1 |
| β-strand | 395-396 | 2 | 5 |
| β-strand | 403-407 | 5 | 5 |
| β-strand | 417-421 | 5 | 5 |
| β-strand | 424-427 | 4 | 4 |
| β-strand | 430-433 | 4 | 4 |
| β-strand | 436-440 | 5 | 5 |
| β-strand | 441 | 1 | 2 |
| α-helix | 445-449 | 5 | |
| β-strand | 450-452 | 3 | 1 |
| β-strand | 460-464 | 5 | 1 |
| β-strand | 471-482 | 12 | 1 |
| α-helix | 487-489 | 3 | |
| β-strand | 501-509 | 9 | 1 |
| α-helix | 511-514 | 4 | |
| β-strand | 526-529 | 4 | 6 |
| β-strand | 534-537 | 4 | 6 |
| α-helix | 541-548 | 8 | |
| α-helix | 558-560 | 3 | |
| β-strand | 565-571 | 7 | 6 |
| β-strand | 577-586 | 10 | 6 |
| α-helix | 598-600 | 3 | |
| β-strand | 601-603 | 3 | 6 |
| β-strand | 610-620 | 11 | 6 |
| α-helix | 623-625 | 3 | |
| α-helix | 629-658 | 30 | |
| α-helix | 665-675 | 11 | |
| α-helix | 686-705 | 20 | |
| α-helix | 708-738 | 31 | |
| α-helix | 758-780 | 23 | |
| α-helix | 787-789 | 3 | |
| α-helix | 791-792 | 2 | |
| α-helix | 795-820 | 26 | |
| β-strand | 823-826 | 4 | 7 |
| β-strand | 834-837 | 4 | 7 |
| α-helix | 840-842 | 3 | |
| β-strand | 844-848 | 5 | 6 |
| α-helix | 866-882 | 17 | |
| β-strand | 889-893 | 5 | 6 |
| β-strand | 901 | 1 | 8 |
| β-strand | 908 | 1 | 8 |
| β-strand | 910-912 | 3 | 9 |
| β-strand | 954 | 1 | 9 |
| β-strand | 959-964 | 6 | 9 |
| β-strand | 970-975 | 6 | 9 |
| α-helix | 976-989 | 14 | |
| α-helix | 994-996 | 3 | |
| β-strand | 998-1005 | 8 | 10 |
| β-strand | 1010-1014 | 5 | 10 |
| α-helix | 1021-1025 | 5 | |
| β-strand | 1029-1031 | 3 | 10 |
| β-strand | 1037-1041 | 5 | 10 |
| α-helix | 1058-1070 | 13 | |
| β-strand | 1074-1078 | 5 | 10 |
| β-strand | 1090-1092 | 3 | 10 |
| α-helix | 1093-1111 | 19 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| CRISPR-associated endonuclease C2c1 | A | protein | 1137 | Alicyclobacillus acidoterrestris | T0D7A2 (AlphaFold model) |
| RNA (60-mer) | B | RNA | 112 | Alicyclobacillus acidoterrestris ATCC 49025 |
>5WQE_1 CRISPR-associated endonuclease C2c1 (chains A) MAVKSIKVKLRLDDMPEIRAGLWKLHKEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQE CDKTAEECKAELLERLRARQVENGHRGPAGSDDELLQLARQLYELLVPQAIGAKGDAQQI ARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKEKAETRKSADRTADVLR ALADFGLKPLMRVYTDSEMSSVEWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGQ EYAKLVEQKNRFEQKNFVGQEHLVHLVNQLQQDMKEASPGLESKEQTAHYVTGRALRGSD KVFEKWGKLAPDAPFDLYDAEIKNVQRRNTRRFGSHDLFAKLAEPEYQALWREDASFLTR YAVYNSILRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGERRHAIRF HKLLKVENGVAREVDDVTVPISMSEQLDNLLPRDPNEPIALYFRDYGAEQHFTGEFGGAK IQCRRDQLAHMHRRRGARDVYLNVSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFD KLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSKGRVPF FFPIKGNDNLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGS EDVGRRERSWAKLIEQPVDAANHMTPDWREAFENELQKLKSLHGICSDKEWMDAVYESVR RVWRHMGKQVRDWRKDVRSGERPKIRGYAKDVVGGNSIEQIEYLERQYKFLKSWSFFGKV SGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDERGKGKWVAKYP PCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELINQAQVHDLLVGTMYAAFSSR FDARTGAPGIRCRRVPARCTQEHNPEPFPWWLNKFVVEHTLDACPLRADDLIPTGEGEIF VSPFSAEEGDFHQIHADLNAAQNLQQRLWSDFDISQIRLRCDWGEVDGELVLIPRLTGKR TADSYSNKVFYTNTGVTYYERERGKKRRKVFAQEKLSEEEAELLVEADEAREKSVVLMRD PSGIINRGNWTRQKEFWSMVNQRIEGYLVKQIRSRVPLQDSACENTGDILEHHHHHH
>5WQE_2 RNA (60-MER) (chains B) GGUCUAGAGGACAGAAUUUUUCAACGGGUGUGCCAAUGGCCACUUUCCAGGUGGCAAAGC CCGUUGAGCUUCUCAAAUCUGAGAAGUGGCACGAGAAGGGGAGGAGAAAGUA
C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism. Liu, L., Chen, P., Wang, M. et al. Mol Cell (2017) 65:310-322. DOI 10.1016/j.molcel.2016.11.040 · PubMed
Other PDB entries of the same protein (UniProt T0D7A2 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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