Crystal structure of EED [G255D] in complex with EZH2 peptide and EED226 compound. Determined by X-ray diffraction at 2.03 Å resolution. Released 3 May 2017.
Explore 5WUK in 3D Show helices and sheets RCSB PDB PDBe
5WUK contains 11 α-helices and 30 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 82-89 | 8 | 1 |
| α-helix | 94-96 | 3 | |
| β-strand | 98-101 | 4 | 2 |
| α-helix | 106 | 1 | |
| β-strand | 111-117 | 7 | 2 |
| β-strand | 120-126 | 7 | 2 |
| α-helix | 128-130 | 3 | |
| β-strand | 132-139 | 8 | 2 |
| β-strand | 147-154 | 8 | 3 |
| β-strand | 161-167 | 7 | 3 |
| β-strand | 172-176 | 5 | 3 |
| β-strand | 181-186 | 6 | 3 |
| β-strand | 193-198 | 6 | 4 |
| β-strand | 205-210 | 6 | 4 |
| β-strand | 215-219 | 5 | 4 |
| β-strand | 224-229 | 6 | 4 |
| β-strand | 239-244 | 6 | 5 |
| β-strand | 250-255 | 6 | 5 |
| β-strand | 260-264 | 5 | 5 |
| α-helix | 268-279 | 12 | |
| α-helix | 288-289 | 2 | |
| β-strand | 292-294 | 3 | 4 |
| β-strand | 299-301 | 3 | 5 |
| β-strand | 311-315 | 5 | 6 |
| β-strand | 318-322 | 5 | 6 |
| β-strand | 327-333 | 7 | 6 |
| α-helix | 340-342 | 3 | |
| β-strand | 350-357 | 8 | 6 |
| β-strand | 368-370 | 3 | 7 |
| β-strand | 376-380 | 5 | 7 |
| β-strand | 386-390 | 5 | 7 |
| α-helix | 396-398 | 3 | |
| β-strand | 400 | 1 | 7 |
| α-helix | 401-403 | 3 | |
| β-strand | 404 | 1 | 7 |
| β-strand | 413-418 | 6 | 1 |
| β-strand | 424-429 | 6 | 1 |
| β-strand | 433-439 | 7 | 1 |
| α-helix | 440 | 1 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 42-61 | 20 | |
| α-helix | 65-67 | 3 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Polycomb protein EED | A | protein | 366 | Homo sapiens | O75530 (AlphaFold model) |
| Histone-lysine N-methyltransferase EZH2 | B | protein | 28 | Homo sapiens | Q15910 (AlphaFold model) |
>5WUK_1 Polycomb protein EED (chains A) KKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLL QSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINE LKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCD MDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWL GDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQK MLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIW RWDRLR
>5WUK_2 Histone-lysine N-methyltransferase EZH2 (chains B) MFSSNRQKILERTEILNQEWKQRRIQPV
| ID | Name | Formula | Copies |
|---|---|---|---|
| 73K | N-(furan-2-ylmethyl)-8-(4-methylsulfonylphenyl)-[1,2,4]triazolo[4,3-c]pyrimidin… | C17 H15 N5 O3 S | 1 |
Water and common crystallization additives (GOL) are not listed.
Split luciferase-based biosensors for characterizing EED binders. Li, L., Feng, L., Shi, M. et al. Anal Biochem (2017) 522:37-45. DOI 10.1016/j.ab.2017.01.014 · PubMed
Other PDB entries of the same protein (UniProt O75530 (AlphaFold model), which also has an AlphaFold model), best resolution first:
MolViewer shows 5WUK directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.