5XAU: Integrin binding fragment of laminin-511

Crystal structure of integrin binding fragment of laminin-511. Determined by X-ray diffraction at 1.8 Å resolution. Released 20 Sept 2017.

Method: X-ray diffraction
Resolution: 1.8 Å
Organism: Homo sapiens
Chains: 6
Atoms: 11,975
Mol. weight: 184.95 kDa
Ligands: NAG, CA
Released: 20 Sept 2017

Explore 5XAU in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5XAU contains 42 α-helices and 84 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 42 β-strands

Element	Residues	Length	Sheet
α-helix	2677-2700	24
α-helix	2707-2731	25
β-strand	2737-2740	4	1
β-strand	2744-2747	4	2
α-helix	2748-2750	3
α-helix	2755-2758	4
β-strand	2759-2766	8	1
β-strand	2780-2786	7	2
β-strand	2794-2800	7	2
β-strand	2803-2809	7	2
β-strand	2815-2819	5	2
β-strand	2829-2836	8	1
β-strand	2839-2846	8	1
β-strand	2852-2859	8	1
β-strand	2870	1	3
β-strand	2873	1	3
β-strand	2875-2878	4	2
α-helix	2889-2891	3
α-helix	2894-2896	3
β-strand	2897-2905	9	1
β-strand	2908-2909	2	1
β-strand	2915-2919	5	2
α-helix	2927-2930	4
α-helix	2938-2942	5
β-strand	2944-2951	8	4
β-strand	2952-2955	4	5
β-strand	2963-2972	10	4
β-strand	2977-2984	8	5
β-strand	2987-2994	8	5
β-strand	2997-3003	7	5
β-strand	3009-3010	2	5
α-helix	3011-3013	3
α-helix	3016-3019	4
β-strand	3025-3032	8	4
β-strand	3038-3043	6	4
β-strand	3046-3052	7	4
α-helix	3057-3059	3
β-strand	3062-3065	4	5
α-helix	3070-3072	3
α-helix	3075-3080	6
β-strand	3088-3096	9	4
β-strand	3099-3100	2	4
α-helix	3103-3105	3
β-strand	3109-3113	5	4
α-helix	3117-3120	4
β-strand	3124-3136	13	6
β-strand	3147-3154	8	6
β-strand	3160-3167	8	6
β-strand	3170-3177	8	6
β-strand	3180-3185	6	6
β-strand	3188-3191	4	6
β-strand	3202-3209	8	6
β-strand	3212-3217	6	6
β-strand	3220-3225	6	6
α-helix	3226-3229	4
β-strand	3243-3246	4	6
α-helix	3256-3258	3
β-strand	3259-3267	9	6
β-strand	3285-3290	6	6

Chains B and E: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	1717-1784	68

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	1536-1552	17
α-helix	1554-1595	42
α-helix	1597	1

Chain D: 18 helices, 42 β-strands

Element	Residues	Length	Sheet
α-helix	2674-2687	14
α-helix	2690-2700	11
α-helix	2704-2731	28
β-strand	2737-2740	4	7
β-strand	2744-2747	4	8
α-helix	2748-2750	3
α-helix	2755-2758	4
β-strand	2759-2766	8	7
β-strand	2780-2786	7	8
β-strand	2794-2800	7	8
β-strand	2803-2809	7	8
β-strand	2816-2819	4	8
β-strand	2829-2836	8	7
β-strand	2839-2846	8	7
β-strand	2852-2859	8	7
β-strand	2870	1	9
β-strand	2873	1	9
β-strand	2875-2878	4	8
α-helix	2889-2891	3
α-helix	2894-2896	3
β-strand	2897-2905	9	7
β-strand	2908-2909	2	7
β-strand	2915-2919	5	8
α-helix	2927-2930	4
α-helix	2938-2942	5
β-strand	2944-2951	8	10
β-strand	2952-2955	4	11
α-helix	2958-2960	3
β-strand	2964-2972	9	10
β-strand	2977-2984	8	11
β-strand	2987-2994	8	11
β-strand	2997-3003	7	11
β-strand	3009-3010	2	11
α-helix	3011-3013	3
α-helix	3016-3019	4
β-strand	3025-3032	8	10
β-strand	3038-3043	6	10
β-strand	3046-3052	7	10
α-helix	3057-3059	3
β-strand	3062-3065	4	11
α-helix	3070-3072	3
α-helix	3075-3080	6
β-strand	3088-3096	9	10
β-strand	3099-3100	2	10
α-helix	3103-3105	3
β-strand	3109-3113	5	10
α-helix	3117-3120	4
β-strand	3124-3136	13	12
β-strand	3147-3154	8	12
β-strand	3160-3166	7	12
β-strand	3171-3177	7	12
β-strand	3180-3185	6	12
β-strand	3188-3191	4	12
β-strand	3202-3208	7	12
β-strand	3212-3217	6	12
β-strand	3220-3226	7	12
β-strand	3243-3246	4	12
α-helix	3256-3258	3
β-strand	3259-3267	9	12
β-strand	3285-3290	6	12

Chain F: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	1554-1595	42
α-helix	1597	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Laminin subunit alpha-5	A, D	protein	674	Homo sapiens	O15230 (AlphaFold model)
Laminin subunit beta-1	B, E	protein	74	Homo sapiens	P07942 (AlphaFold model)
Laminin subunit gamma-1	C, F	protein	83	Homo sapiens	P11047 (AlphaFold model)

Sequence of entity 1 (A, D), FASTA

>5XAU_1 Laminin subunit alpha-5 (chains A, D)
GENVERWQGQYEGLRGQDLGQAVLDAGHSVSTLEKTLPQLLAKLSILENRGVHNASLALS
ASIGRVRELCAQARGAASKVKVPMKFNGRSGVQLRTPRDLADLAAYTALKFYLQGPEPEP
GQGTEDRFVMYMGSRQATGDYMGVSLRDKKVHWVYQLGEAGPAVLSIDEDIGEQFAAVSL
DRTLQFGHMSVTVERQMIQETKGDTVAPGAEGLLNLRPDDFVFYVGGYPSTFTPPPLLRF
PGYRGCIEMDTLNEEVVSLYNFERTFQLDTAVDRPCARSKSTGDPWLTDGSYLDGTGFAR
ISFDSQISTTKRFEQELRLVSYSGVLFFLKQQSQFLCLAVQEGSLVLLYDFGAGLKKAVP
LQPPPPLTSASKAIQVFLLGGSRKRVLVRVERATVYSVEQDNDLELADAYYLGGVPPDQL
PPSLRRLFPTGGSVRGCVKGIKALGKYVDLKRLNTTGVSAGCTADLLVGRAMTFHGHGFL
RLALSNVAPLTGNVYSGFGFHSAQDSALLYYRASPDGLCQVSLQQGRVSLQLLRTEVKTQ
AGFADGAPHYVAFYSNATGVWLYVDDQLQQMKPHRGPPPELQPQPEGPPRLLLGGLPESG
TIYNFSGCISNVFVQRLLGPQRVFDLQQNLGSVNVSTGCAPALQAQTPGLGPRGLQATAR
KASRRSRQPARHPA

Sequence of entity 2 (B, E), FASTA

>5XAU_2 Laminin subunit beta-1 (chains B, E)
GDARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRSLL
KDISQKVAVYSTCL

Sequence of entity 3 (C, F), FASTA

>5XAU_3 Laminin subunit gamma-1 (chains C, F)
GDTVDLNKLNEIEGTLNKAKDEMKVSDLDRKVSDLENEAKKQEAAIMDYNRDIEEIMKCI
RNLEDIRKTLPSGCFNTPSIEKP

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	7
CA	Calcium ion	Ca	2

Primary citation

Mechanistic basis for the recognition of laminin-511 by alpha 6 beta 1 integrin. Takizawa, M., Arimori, T., Taniguchi, Y. et al. Sci Adv (2017) 3:e1701497-e1701497. DOI 10.1126/sciadv.1701497 · PubMed

Other PDB entries of the same protein (UniProt O15230 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7CEC 3.9 Å, Structure of alpha6beta1 integrin in complex with laminin-511

Browse structure collections

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