5XWY: PDB entry 5XWY

Electron cryo-microscopy structure of LbuCas13a-crRNA binary complex. Determined by electron microscopy at 3.2 Å resolution. Released 13 Sept 2017.

Method
Electron microscopy
Resolution
3.2 Å
Organisms
Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b), synthetic construct
Chains
2
Atoms
10,493
Mol. weight
157.42 kDa
Released
13 Sept 2017

Explore 5XWY in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5XWY contains 63 α-helices and 18 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 63 helices, 18 β-strands

ElementResiduesLengthSheet
β-strand10-1341
β-strand18-2141
α-helix30-367
β-strand39-4132
α-helix60-689
β-strand72-7432
β-strand82-8322
α-helix114-1174
α-helix127-1293
α-helix131-14919
β-strand156-16053
β-strand163-16753
α-helix171-1788
α-helix182-1843
α-helix185-19915
α-helix204-21310
α-helix220-23112
α-helix234-2374
α-helix241-25111
α-helix254-2607
α-helix268-27710
α-helix287-2915
α-helix292-30413
α-helix305-3095
α-helix318-3247
α-helix326-35328
β-strand36013
α-helix363-39230
α-helix428-4314
α-helix434-44512
α-helix456-47621
α-helix484-4863
α-helix497-5048
α-helix509-52214
α-helix525-5284
α-helix531-5388
β-strand54314
α-helix553-5553
α-helix563-5664
α-helix584-59815
α-helix599-6046
α-helix605-6073
α-helix616-62712
α-helix639-6435
α-helix651-66414
α-helix676-69419
α-helix708-7136
α-helix717-73014
α-helix738-7447
β-strand74914
α-helix757-76610
α-helix771-78818
α-helix797-8004
α-helix801-8033
α-helix818-8214
β-strand82515
β-strand826-82726
β-strand830-83126
α-helix835-8417
α-helix858-8647
α-helix869-87810
β-strand88215
α-helix884-89411
α-helix897-91216
α-helix922-94524
α-helix948-98235
α-helix987-9893
α-helix1008-101912
α-helix1028-10303
α-helix1034-10407
α-helix1048-10525
α-helix1064-107411
α-helix1075-10773
α-helix1079-10824
α-helix1084-109512
β-strand1099-110467
β-strand1110-111677
α-helix1117-11182
β-strand111918
β-strand113418
α-helix1138-114912

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
A type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, Cas13aAprotein1159Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b)C7NBY4 (AlphaFold model)
RNA (59-mer)BRNA59synthetic construct
Sequence of entity 1 (A), FASTA
>5XWY_1 A type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, Cas13a (chains A)
MKVTKVGGISHKKYTSEGRLVKSESEENRTDERLSALLNMRLDMYIKNPSSTETKENQKR
IGKLKKFFSNKMVYLKDNTLSLKNGKKENIDREYSETDILESDVRDKKNFAVLKKIYLNE
NVNSEELEVFRNDIKKKLNKINSLKYSFEKNKANYQKINENNIEKVEGKSKRNIIYDYYR
ESAKRDAYVSNVKEAFDKLYKEEDIAKLVLEIENLTKLEKYKIREFYHEIIGRKNDKENF
AKIIYEEIQNVNNMKELIEKVPDMSELKKSQVFYKYYLDKEELNDKNIKYAFCHFVEIEM
SQLLKNYVYKRLSNISNDKIKRIFEYQNLKKLIENKLLNKLDTYVRNCGKYNYYLQDGEI
ATSDFIARNRQNEAFLRNIIGVSSVAYFSLRNILETENENDITGRMRGKTVKNNKGEEKY
VSGEVDKIYNENKKNEVKENLKMFYSYDFNMDNKNEIEDFFANIDEAISSIRHGIVHFNL
ELEGKDIFAFKNIAPSEISKKMFQNEINEKKLKLKIFRQLNSANVFRYLEKYKILNYLKR
TRFEFVNKNIPFVPSFTKLYSRIDDLKNSLGIYWKTPKTNDDNKTKEIIDAQIYLLKNIY
YGEFLNYFMSNNGNFFEISKEIIELNKNDKRNLKTGFYKLQKFEDIQEKIPKEYLANIQS
LYMINAGNQDEEEKDTYIDFIQKIFLKGFMTYLANNGRLSLIYIGSDEETNTSLAEKKQE
FDKFLKKYEQNNNIKIPYEINEFLREIKLGNILKYTERLNMFYLILKLLNHKELTNLKGS
LEKYQSANKEEAFSDQLELINLLNLDNNRVTEDFELEADEIGKFLDFNGNKVKDNKELKK
FDTNKIYFDGENIIKHRAFYNIKKYGMLNLLEKIADKAGYKISIEELKKYSNKKNEIEKN
HKMQENLHRKYARPRKDEKFTDEDYESYKQAIENIEEYTHLKNKVEFNELNLLQGLLLRI
LHRLVGYTSIWERDLRFRLKGEFPENQYIEEIFNFENKKNVKYKGGQIVEKYIKFYKELH
QNDEVKINKYSSANIKVLKQEKKDLYIANYIAAFNYIPHAEISLLEVLENLRKLLSYDRK
LKNAVMKSVVDILKEYGFVATFKIGADKKIGIQTLESEKIVHLKNLKKKKLMTDRNSEEL
CKLVKIMFEYKMEEKKSEN
Sequence of entity 2 (B), FASTA
>5XWY_2 RNA (59-MER) (chains B)
GGACCACCCCAAAAAUGAAGGGGACUAAAACACAAAUCUAUCUGAAUAAACUCUUCUUC

Primary citation

The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a. Liu, L., Li, X., Ma, J. et al. Cell (2017) 170:714-726.e10. DOI 10.1016/j.cell.2017.06.050 · PubMed

Other PDB entries of the same protein (UniProt C7NBY4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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