9MVS: AIcrVIA1

Activated Leptotrichia buccalis (Lbu) CRISPR-Cas13a bound to AI-designed anti-CRISPR AIcrVIA1. Determined by electron microscopy at 3.43 Å resolution. Released 25 Feb 2026.

Method
Electron microscopy
Resolution
3.43 Å
Organisms
synthetic construct, Leptotrichia buccalis
Chains
4
Atoms
11,346
Mol. weight
174.95 kDa
Released
25 Feb 2026

Explore 9MVS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9MVS contains 65 α-helices and 18 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 63 helices, 16 β-strands

ElementResiduesLengthSheet
β-strand9-1352
β-strand18-2252
α-helix30-378
β-strand39-4133
α-helix42-443
α-helix55-6814
β-strand72-7653
β-strand79-8353
α-helix107-11812
α-helix124-14926
β-strand155-15844
β-strand165-16844
α-helix171-1788
α-helix185-19915
α-helix202-21312
α-helix220-23112
α-helix233-2353
α-helix236-2405
α-helix241-25010
α-helix254-2607
α-helix266-2727
α-helix273-2775
α-helix285-2884
α-helix291-30313
α-helix304-3096
α-helix319-3224
α-helix326-35429
β-strand36014
α-helix363-37614
α-helix380-39314
β-strand409-41135
β-strand419-42135
α-helix424-4318
α-helix434-44411
α-helix454-47522
α-helix484-4863
α-helix497-50610
α-helix509-52214
α-helix531-54010
α-helix554-5552
α-helix556-56914
α-helix582-59918
α-helix600-6045
α-helix605-6084
α-helix615-62814
α-helix651-66515
α-helix676-69621
α-helix699-7035
α-helix708-7136
α-helix717-73115
α-helix734-7374
α-helix738-7469
α-helix753-7553
α-helix757-76812
α-helix771-78717
α-helix794-80411
α-helix818-8225
α-helix835-8417
β-strand84816
β-strand85316
α-helix857-8659
α-helix868-87811
α-helix884-91229
α-helix922-94625
α-helix948-98235
α-helix988-9936
α-helix1008-101912
α-helix1024-10296
α-helix1032-104110
α-helix1046-10527
α-helix1064-107411
α-helix1079-10824
α-helix1085-109511
β-strand1098-110367
β-strand1111-111777
α-helix11181
β-strand1119-112248
β-strand1131-113448
α-helix1138-114912
Chain B: 2 helices, 2 β-strands
ElementResiduesLengthSheet
β-strand3-1081
α-helix15-3218
β-strand35-4281
α-helix49-7527

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
AIcrVIA1Bprotein83synthetic construct
CRISPR-associated endoribonuclease Cas13aAprotein1162Leptotrichia buccalisC7NBY4 (AlphaFold model)
crRNACRNA56Leptotrichia buccalis
Guide complementary activator RNA (aRNA)DRNA26synthetic construct
Sequence of entity 1 (B), FASTA
>9MVS_1 AIcrVIA1 (chains B)
MKTIKVDVIVVGDDEELVEEYKKEAELIGKEYGVKIEVEPYFLEEGKFPWLDVDFAYNTT
QEELDKAEKEAKKIAGSHHHHHH
Sequence of entity 2 (A), FASTA
>9MVS_2 CRISPR-associated endoribonuclease Cas13a (chains A)
SNAMKVTKVGGISHKKYTSEGRLVKSESEENRTDERLSALLNMRLDMYIKNPSSTETKEN
QKRIGKLKKFFSNKMVYLKDNTLSLKNGKKENIDREYSETDILESDVRDKKNFAVLKKIY
LNENVNSEELEVFRNDIKKKLNKINSLKYSFEKNKANYQKINENNIEKVEGKSKRNIIYD
YYRESAKRDAYVSNVKEAFDKLYKEEDIAKLVLEIENLTKLEKYKIREFYHEIIGRKNDK
ENFAKIIYEEIQNVNNMKELIEKVPDMSELKKSQVFYKYYLDKEELNDKNIKYAFCHFVE
IEMSQLLKNYVYKRLSNISNDKIKRIFEYQNLKKLIENKLLNKLDTYVRNCGKYNYYLQD
GEIATSDFIARNRQNEAFLRNIIGVSSVAYFSLRNILETENENDITGRMRGKTVKNNKGE
EKYVSGEVDKIYNENKKNEVKENLKMFYSYDFNMDNKNEIEDFFANIDEAISSIRHGIVH
FNLELEGKDIFAFKNIAPSEISKKMFQNEINEKKLKLKIFRQLNSANVFRYLEKYKILNY
LKRTRFEFVNKNIPFVPSFTKLYSRIDDLKNSLGIYWKTPKTNDDNKTKEIIDAQIYLLK
NIYYGEFLNYFMSNNGNFFEISKEIIELNKNDKRNLKTGFYKLQKFEDIQEKIPKEYLAN
IQSLYMINAGNQDEEEKDTYIDFIQKIFLKGFMTYLANNGRLSLIYIGSDEETNTSLAEK
KQEFDKFLKKYEQNNNIKIPYEINEFLREIKLGNILKYTERLNMFYLILKLLNHKELTNL
KGSLEKYQSANKEEAFSDQLELINLLNLDNNRVTEDFELEADEIGKFLDFNGNKVKDNKE
LKKFDTNKIYFDGENIIKHRAFYNIKKYGMLNLLEKIADKAGYKISIEELKKYSNKKNEI
EKNHKMQENLHRKYARPRKDEKFTDEDYESYKQAIENIEEYTHLKNKVEFNELNLLQGLL
LRILHRLVGYTSIWERDLRFRLKGEFPENQYIEEIFNFENKKNVKYKGGQIVEKYIKFYK
ELHQNDEVKINKYSSANIKVLKQEKKDLYIRNYIAHFNYIPHAEISLLEVLENLRKLLSY
DRKLKNAVMKSVVDILKEYGFVATFKIGADKKIGIQTLESEKIVHLKNLKKKKLMTDRNS
EELCKLVKIMFEYKMEEKKSEN
Sequence of entity 3 (C), FASTA
>9MVS_3 crRNA (chains C)
UAGACCACCCCAAAAAUGAAGGGGACUAAAACUUUCUUUCUUUCCUUUUUCUGCCG
Sequence of entity 4 (D), FASTA
>9MVS_4 Guide complementary activator RNA (aRNA) (chains D)
CGGCAGAAAAAGGAAAGAAAGAAACC

Primary citation

De novo design of potent CRISPR-Cas13 inhibitors. Taveneau, C., Chai, H.X., D'Silva, J. et al. Nat Chem Biol (2026) 22:1342-1350. DOI 10.1038/s41589-025-02136-3 · PubMed

Other PDB entries of the same protein (UniProt C7NBY4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 9MVS directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.