6E7B: Tubulin beta-3 chain

13-pf 3-start GMPCPP-human alpha1B/beta3 microtubules. Determined by electron microscopy at 3.5 Å resolution. Released 10 Oct 2018.

Method: Electron microscopy
Resolution: 3.5 Å
Organism: Homo sapiens
Chains: 2
Atoms: 6,774
Mol. weight: 97.57 kDa
Ligands: GTP, G2P, MG
Released: 10 Oct 2018

Explore 6E7B in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6E7B contains 43 α-helices and 41 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	5-9	5	9
α-helix	10-27	18
β-strand	35	1	10
α-helix	48-50	3
β-strand	53-56	4	10
β-strand	60-63	4	10
β-strand	65-68	4	9
β-strand	69	1	11
α-helix	72-79	8
β-strand	94	1	11
α-helix	103-104	2
α-helix	105-109	5
α-helix	111-113	3
α-helix	115-128	14
β-strand	134-137	4	9
α-helix	145-149	5
α-helix	150-160	11
β-strand	165-172	8	9
α-helix	173-174	2
α-helix	183-196	14
β-strand	200-205	6	9
α-helix	206-215	10
α-helix	224-243	20
β-strand	248	1	12
α-helix	252-259	8
β-strand	272	1	13
α-helix	278-283	6
α-helix	288-295	8
α-helix	311	1
β-strand	312-314	3	14
β-strand	315-321	7	13
α-helix	325-337	13
β-strand	343	1	14
β-strand	351-354	4	13
β-strand	355	1	12
α-helix	358-361	4
β-strand	373-377	5	13
β-strand	380-381	2	14
α-helix	384-401	18
α-helix	405-409	5
α-helix	415-436	22

Chain B: 21 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	3-4	2	1
β-strand	7	1	2
α-helix	10-27	18
β-strand	30	1	3
β-strand	35	1	4
β-strand	36	1	3
α-helix	42-45	4
α-helix	47-49	3
β-strand	51-54	4	4
β-strand	58-61	4	4
β-strand	64	1	2
β-strand	65-66	2	5
α-helix	71-78	8
α-helix	87-89	3
β-strand	90-91	2	5
α-helix	101-105	5
α-helix	107-126	20
β-strand	130-132	3	1
β-strand	134-138	5	6
α-helix	143-147	5
α-helix	148-158	11
β-strand	165-170	6	6
α-helix	181-192	12
β-strand	199-200	2	6
β-strand	202-203	2	6
α-helix	204-210	7
α-helix	211-215	5
α-helix	222-241	20
β-strand	246	1	7
α-helix	250-257	8
β-strand	265-266	2	6
α-helix	286-293	8
α-helix	309	1
β-strand	310-316	7	8
α-helix	323-336	14
β-strand	341	1	8
β-strand	349-352	4	8
β-strand	353	1	7
α-helix	357-358	2
β-strand	367-371	5	8
α-helix	374-390	17
α-helix	396-401	6
α-helix	406-425	20

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Tubulin beta-3 chain	B	protein	426	Homo sapiens	Q13509 (AlphaFold model)
Tubulin alpha-1B chain	A	protein	437	Homo sapiens	P68363 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>6E7B_1 Tubulin beta-3 chain (chains B)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVGDSDLQLERISVYYNEASSHKYV
PRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVV
EPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMM
AACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRG
LKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQ

Sequence of entity 2 (A), FASTA

>6E7B_2 Tubulin alpha-1B chain (chains A)
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGV

Ligands and cofactors

ID	Name	Formula	Copies
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
G2P	Phosphomethylphosphonic acid guanylate ester	C11 H18 N5 O13 P3	1
MG	Magnesium ion	Mg	2

Primary citation

Human beta-Tubulin Isotypes Can Regulate Microtubule Protofilament Number and Stability. Ti, S.C., Alushin, G.M., Kapoor, T.M. Dev Cell (2018) 47:175. DOI 10.1016/j.devcel.2018.08.014 · PubMed

Other PDB entries of the same protein (UniProt Q13509 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6S8L 1.8 Å, Structure, Thermodynamics, and Kinetics of Plinabulin Binding to two Tubulin Isotypes
7PJF 1.86 Å, Inhibiting parasite proliferation using a rationally designed anti-tubulin agent
9WD9 2.26 Å, GMPCPP-stabilized human alpha1A/beta3 S239C microtubule
9WD7 2.34 Å, GMPCPP-stabilizsd human alpha1A/beta3 microtubule
9WDA 2.35 Å, Paclitaxel/GMPCPP-stabilized human alpha1A/beta3 microtubule
9WDB 2.39 Å, Paclitaxel/GMPCPP-stabilized human alpha1A/beta3 S239C microtubule
22AK 2.41 Å, GDP human alpha1A/beta3 microtubule
22AJ 2.48 Å, GDP human alpha1A/beta3 S239C microtubule
8VT7 2.66 Å, Structure of the gamma tubulin ring complex nucleated microtubule protofilament.
7Z6S 2.9 Å, MATCAP bound to a human 14 protofilament microtubule
6WSL 3.1 Å, Cryo-EM structure of VASH1-SVBP bound to microtubules
7LXB 3.26 Å, HeLa-tubulin in complex with cryptophycin 52

Browse structure collections

About this viewer

MolViewer shows 6E7B directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.