6HZW: Proton-gated ion channel

THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL 2.22 resolution. Determined by X-ray diffraction at 2.22 Å resolution. Released 19 Dec 2018.

Method: X-ray diffraction
Resolution: 2.22 Å
Organism: Gloeobacter violaceus PCC 7421
Chains: 5
Atoms: 13,965
Mol. weight: 197.32 kDa
Ligands: PLC, LMT
Released: 19 Dec 2018

Explore 6HZW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6HZW contains 63 α-helices and 73 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
α-helix	14-15	2
β-strand	16-31	16	1
β-strand	36-48	13	1
α-helix	50-52	3
α-helix	56-59	4
β-strand	64-65	2	1
β-strand	76-78	3	2
β-strand	81	1	1
β-strand	86-94	9	1
β-strand	99-111	13	1
β-strand	123-133	11	2
β-strand	135	1	3
β-strand	138	1	3
β-strand	140-144	5	1
α-helix	146-148	3
β-strand	150-151	2	1
β-strand	160-176	17	2
β-strand	179-192	14	2
α-helix	197	1
α-helix	198-202	5
α-helix	203-212	10
α-helix	213-217	5
α-helix	221-244	24
α-helix	254-281	28
α-helix	285-314	30

Chains B and E: 13 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
α-helix	14-15	2
β-strand	16-31	16	4
β-strand	36-48	13	4
α-helix	50-52	3
α-helix	56-59	4
β-strand	64-65	2	4
α-helix	68-70	3
β-strand	76-78	3	5
β-strand	81	1	4
β-strand	86-94	9	4
β-strand	99-111	13	4
β-strand	123-133	11	5
β-strand	135	1	6
β-strand	138	1	6
β-strand	140-144	5	4
α-helix	146-148	3
β-strand	150-151	2	4
β-strand	157	1	5
β-strand	160-176	17	5
β-strand	179-192	14	5
α-helix	197	1
α-helix	198-202	5
α-helix	203-212	10
α-helix	213-217	5
α-helix	221-244	24
α-helix	254-281	28
α-helix	285-314	30

Chain C: 13 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
α-helix	14-15	2
β-strand	16-31	16	7
β-strand	36-48	13	7
α-helix	50-52	3
α-helix	56-59	4
β-strand	64-65	2	7
α-helix	68-70	3
β-strand	76-78	3	8
β-strand	81	1	7
β-strand	86-94	9	7
β-strand	99-111	13	7
β-strand	123-133	11	8
β-strand	135	1	9
β-strand	138	1	9
β-strand	140-144	5	7
α-helix	146-148	3
β-strand	150-151	2	7
β-strand	160-176	17	8
β-strand	179-192	14	8
α-helix	197	1
α-helix	198-202	5
α-helix	203-212	10
α-helix	213-217	5
α-helix	221-244	24
α-helix	254-281	28
α-helix	285-314	30

Chain D: 12 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
α-helix	14-15	2
β-strand	16-31	16	10
β-strand	36-48	13	10
α-helix	50-52	3
α-helix	56-59	4
β-strand	64-65	2	10
β-strand	76-78	3	11
β-strand	81	1	10
β-strand	86-94	9	10
β-strand	99-111	13	10
β-strand	123-133	11	11
β-strand	135	1	12
β-strand	138	1	12
β-strand	140-144	5	10
α-helix	146-148	3
β-strand	150-151	2	10
β-strand	157	1	11
β-strand	160-176	17	11
β-strand	179-192	14	11
α-helix	197	1
α-helix	198-202	5
α-helix	203-212	10
α-helix	213-217	5
α-helix	221-244	24
α-helix	254-281	28
α-helix	285-314	30

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Proton-gated ion channel	A, B, C, D, E	protein	317	Gloeobacter violaceus PCC 7421	Q7NDN8 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E), FASTA

>6HZW_1 Proton-gated ion channel (chains A, B, C, D, E)
GQDMVSPPPPIADEPLTVNTGIYLIECYSLDDKAETFKVNAFLSLSWKDRRLAFDPVRSG
VRVKTYEPEAIWIPEIRFVNVENARDADVVDISVSPDGTVQYLERFSARVLSPLDFRRYP
FDSQTLHIYLIVRSVDTRNIVLAVDLEKVGKNDDVFLTGWDIESFTAVVKPANFALEDRL
ESKLDYQLRISRQYFSYIPNIILPMLFILFISWTAFWSTSYEANVTLVVSTLIAHIAFNI
LVETNLPKTPYMTYTGAIIFMIYLFYFVAVIEVTVQHYLKVESQPARAASITRASRIAFP
VVFLLANIILAFLFFGF

Ligands and cofactors

ID	Name	Formula	Copies
PLC	Diundecyl phosphatidyl choline	C32 H65 N O8 P	15
LMT	Dodecyl-beta-D-maltoside	C24 H46 O11	11

Water and common crystallization additives (ACT, CL, NA) are not listed.

Primary citation

Electrostatics, proton sensor, and networks governing the gating transition in GLIC, a proton-gated pentameric ion channel. Hu, H., Ataka, K., Menny, A. et al. Proc Natl Acad Sci U S A (2018) 115:E12172-E12181. DOI 10.1073/pnas.1813378116 · PubMed

Other PDB entries of the same protein (UniProt Q7NDN8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9LBD 2.14 Å, Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC F116A mutant at pH 2.5
3IGQ 2.3 Å, Crystal structure of the extracellular domain of a bacterial pentameric ligand-gated ion…
6F0U 2.35 Å, GLIC mutant E35A
4HFI 2.4 Å, The GLIC pentameric Ligand-Gated Ion Channel at 2.4 A resolution
6F0R 2.5 Å, GLIC mutant E82Q
6F0Z 2.5 Å, GLIC mutant D88N
6HJZ 2.5 Å, Xray structure of GLIC in complex with succinate
6HZ1 2.5 Å, The glic pentameric ligand-gated ion channel mutant E243C
6HY5 2.58 Å, The glic pentameric ligand-gated ion channel mutant Q193C
3TLW 2.6 Å, The GLIC pentameric Ligand-Gated Ion Channel Loop2-21' oxidized mutant in a…
5IUX 2.6 Å, GLIC-V135C bimane labelled X-ray structure
6F16 2.6 Å, GLIC mutant H277Q

Browse structure collections

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