6MNO: 6235 TCR

6235 TCR bound to I-Ab Padi4. Determined by X-ray diffraction at 2.9 Å resolution. Released 3 Jul 2019.

Method: X-ray diffraction
Resolution: 2.9 Å
Organism: Mus musculus
Chains: 4
Atoms: 6,133
Mol. weight: 95.27 kDa
Released: 3 Jul 2019

Explore 6MNO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6MNO contains 24 α-helices and 79 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	9
β-strand	10-14	5	10
α-helix	18	1
β-strand	19-25	7	9
β-strand	32-38	7	10
β-strand	45-51	7	10
β-strand	56-59	4	9
β-strand	62-67	6	9
β-strand	72-77	6	9
α-helix	82-84	3
β-strand	86-90	5	10
β-strand	91-92	2	11
β-strand	93	1	10
α-helix	95-97	3
β-strand	103-104	2	11
β-strand	108-113	6	10
α-helix	114-115	2
β-strand	122-125	4	12
β-strand	127	1	13
β-strand	135-140	6	12
β-strand	147	1	14
β-strand	156-159	4	12
β-strand	163-164	2	15
β-strand	176-180	5	12
β-strand	195	1	14
β-strand	201	1	12

Chain B: 7 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	2-5	4	16
β-strand	8-12	5	17
β-strand	17-18	2	18
β-strand	20-23	4	16
β-strand	29-36	8	17
β-strand	40-47	8	17
β-strand	54-55	2	17
β-strand	62-65	4	18
β-strand	71-72	2	16
β-strand	73-76	4	18
α-helix	81-83	3
β-strand	85-92	8	17
β-strand	100-101	2	17
β-strand	105-110	6	17
α-helix	113-115	3
β-strand	117	1	19
α-helix	118-119	2
β-strand	120-124	5	15
β-strand	125	1	13
α-helix	126-127	2
α-helix	128-134	7
β-strand	136-146	11	15
β-strand	147	1	19
β-strand	151-157	7	20
β-strand	160-162	3	20
β-strand	166-168	3	15
β-strand	173-174	2	15
β-strand	184-193	10	15
α-helix	194-197	4
β-strand	203-210	8	20
β-strand	213	1	21
α-helix	224-225	2
β-strand	227	1	21
β-strand	229-236	8	20

Chain C: 3 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	4-15	12	1
β-strand	19-26	8	1
β-strand	29-35	7	1
β-strand	40-43	4	1
β-strand	53	1	2
α-helix	56-76	21
α-helix	80-84	5
β-strand	88	1	3
β-strand	91-93	3	4
β-strand	103-112	10	4
β-strand	118-120	3	5
β-strand	131-134	4	4
α-helix	136-137	2
β-strand	138-139	2	4
β-strand	145-153	9	4
β-strand	162-166	5	5
β-strand	174-177	4	5

Chain D: 10 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	-24	1	2
α-helix	-18--16	3
β-strand	7-18	12	1
β-strand	23-32	10	1
β-strand	35-41	7	1
β-strand	46-49	4	1
α-helix	52-54	3
α-helix	55-63	9
α-helix	65-74	10
α-helix	75-80	6
α-helix	81-82	2
α-helix	83-87	5
α-helix	88-89	2
α-helix	91-93	3
β-strand	96	1	6
α-helix	97-98	2
β-strand	99-104	6	7
β-strand	115-123	9	7
β-strand	124	1	6
β-strand	129-134	6	8
β-strand	138	1	8
β-strand	143-145	3	7
β-strand	149-150	2	7
β-strand	156-163	8	7
β-strand	171-177	7	8
β-strand	185-190	6	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
H-2 class II histocompatibility antigen, A-B alpha chain	C	protein	179	Mus musculus	P14434 (AlphaFold model)
Padi4 (92-105) peptide and MHC Class II I-Ab beta chain	D	protein	217	Mus musculus	P14483 (AlphaFold model), Q9Z183 (AlphaFold model)
6235 TCR alpha chain	A	protein	208	Mus musculus
6235 TCR beta chain	B	protein	239	Mus musculus

Sequence of entity 1 (C), FASTA

>6MNO_1 H-2 class II histocompatibility antigen, A-B alpha chain (chains C)
IEADHVGTYGISVYQSPGDIGQYTFEFDGDELFYVDLDKKETVWMLPEFGQLASFDPQGG
LQNIAVVKHNLGVLTKRSNSTPATNEAPQATVFPKSPVLLGQPNTLICFVDNIFPPVINI
TWLRNSKSVADGVYETSFFVNRDYSFHKLSYLTFIPSDDDIYDCKVEHWGLEEPVLKHW

Sequence of entity 2 (D), FASTA

>6MNO_2 Padi4 (92-105) peptide and MHC Class II I-Ab beta chain (chains D)
RVSYYGPKTSPVQGGGGSLVPRGSGGGGSERHFVYQFMGECYFTNGTQRIRYVTRYIYNR
EEYVRYDSDVGEHRAVTELGRPDAEYWNSQPEILERTRAELDTVCRHNYEGPETHTSLRR
LEQPNVVISLSRTEALNHHNTLVCSVTDFYPAKIKVRWFRNGQEETVGVSSTQLIRNGDW
TFQVLVMLEMTPRRGEVYTCHVEHPSLKSPITVEWRA

Sequence of entity 3 (A), FASTA

>6MNO_3 6235 TCR alpha chain (chains A)
MQQVRQSPQSLTVWEGETAILNCSYENSAFDYFPWYQQFPGEGPALLIAIRSVSDKKEDG
RFTIFFNKREKKLSLHITDSQPGDSATYFCAASETGANTGKLTFGHGTILRVHPNIQNPD
PAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWS
NKSDFACANAFNNSIIPEDTFFPSPESS

Sequence of entity 4 (B), FASTA

>6MNO_4 6235 TCR beta chain (chains B)
AVTQSPRNKVAVTGGKVTLSCNQTNNHNNMYWYRQDTGHGLRLIHYSYGAGSTEKGDIPD
GYKASRPSQENFSLILELATPSQTSVYFCASGDFWGDTLYFGAGTRLSVLEDLKNVFPPE
VAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALN
DSRYALSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRA

Primary citation

A temporal thymic selection switch and ligand binding kinetics constrain neonatal Foxp3+Tregcell development. Stadinski, B.D., Blevins, S.J., Spidale, N.A. et al. Nat Immunol (2019) 20:1046-1058. DOI 10.1038/s41590-019-0414-1 · PubMed

Other PDB entries of the same protein (UniProt P14434 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8VQ8 2.01 Å, Immune receptor complex
8RAL 2.1 Å, CL3E peptide bound to the I-Ab murine MHC class II receptor
1MUJ 2.15 Å, Crystal structure of murine class II MHC I-Ab in complex with a human CLIP peptide
4P23 2.25 Å, J809.B5 TCR bound to IAb/3K
1LNU 2.5 Å, Crystal structure of class II MHC molecule iab bound to EALPHA3K peptide
3C5Z 2.55 Å, Crystal structure of mouse MHC class II I-Ab/3K peptide complexed with mouse TCR B3K506
6MNG 2.66 Å, 4738 TCR bound to IAb Padi4
3RDT 2.7 Å, Crystal Structure of 809.B5 TCR complexed with MHC Class II I-Ab/3k peptide
6MNN 2.83 Å, 6236 TCR bound to I-Ab Padi4
4P46 2.85 Å, J809.B5 Y31A TCR bound to IAb3K
9AUD 2.9 Å, Immune receptor complex
3C60 3.05 Å, Crystal structure of mouse MHC class II I-Ab/3K peptide complexed with mouse TCR YAe62

Browse structure collections

About this viewer

MolViewer shows 6MNO directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.