6N5W: PDB entry 6N5W

Crystal structure of the Ca2+/CaM complex with independent peptides of Kv7.4 (KCNQ4) A & B domains. Determined by X-ray diffraction at 2.15 Å resolution. Released 13 Mar 2019.

Method: X-ray diffraction
Resolution: 2.15 Å
Organism: Homo sapiens
Chains: 3
Atoms: 1,522
Mol. weight: 23.22 kDa
Ligands: CA
Released: 13 Mar 2019

Explore 6N5W in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6N5W contains 11 α-helices and 4 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	339-353	15

Chain B: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	527-548	22

Chain C: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	6-19	14
β-strand	26-27	2	1
α-helix	29-38	10
α-helix	45-55	11
β-strand	63-64	2	1
α-helix	65-74	10
α-helix	81-90	10
β-strand	99-101	3	2
α-helix	102-111	10
α-helix	115-117	3
α-helix	118-128	11
β-strand	135-137	3	2
α-helix	138-145	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Potassium voltage-gated channel subfamily KQT member 4	A	protein	27	Homo sapiens	P56696 (AlphaFold model)
Potassium voltage-gated channel subfamily KQT member 4	B	protein	26	Homo sapiens	P56696 (AlphaFold model)
Calmodulin-1	C	protein	149	Homo sapiens	P0DP23 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6N5W_1 Potassium voltage-gated channel subfamily KQT member 4 (chains A)
EKRRMPAANLIQAAWRLYSTDMSRAYL

Sequence of entity 2 (B), FASTA

>6N5W_2 Potassium voltage-gated channel subfamily KQT member 4 (chains B)
DDIMPAVKTVIRSIRILKFLVAKRKF

Sequence of entity 3 (C), FASTA

>6N5W_3 Calmodulin-1 (chains C)
MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE
EVDEMIREADIDGDGQVNYEEFVQMMTAK

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	2

Primary citation

A mutually induced conformational fit underlies Ca2+-directed interactions between calmodulin and the proximal C terminus of KCNQ4 K+channels. Archer, C.R., Enslow, B.T., Taylor, A.B. et al. J Biol Chem (2019) 294:6094-6112. DOI 10.1074/jbc.RA118.006857 · PubMed

Other PDB entries of the same protein (UniProt P56696 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2OVC 2.07 Å, Crystal structure of a coiled-coil tetramerization domain from Kv7.4 channels
6B8P 2.2 Å, Crystal Structure of the Mg2+/CaM:Kv7.4 (KCNQ4) AB domain complex
6B8N 2.2 Å, Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak
6B8L 2.3 Å, Crystal Structure of the Apo/CaM:Kv7.4 (KCNQ4) AB Domain Complex
6B8M 2.3 Å, Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 1 mM CaCl2 soak
7BYL 2.5 Å, Cryo-EM structure of human KCNQ4
4GOW 2.6 Å, Crystal Structure of Ca2+/CaM:Kv7.4 (KCNQ4) B helix complex
7VNP 2.79 Å, Structure of human KCNQ4-ML213 complex with PIP2
7VNR 2.8 Å, Structure of human KCNQ4-ML213 complex in digitonin
7VNQ 2.96 Å, Structure of human KCNQ4-ML213 complex in nanodisc
7BYM 3.1 Å, Cryo-EM structure of human KCNQ4 with retigabine
7BYN 3.3 Å, Cryo-EM structure of human KCNQ4 with linopirdine

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