Cryo-EM structure of the complex between human TBK1 and chicken STING. Determined by electron microscopy at 3.3 Å resolution. Released 6 Mar 2019.
Explore 6NT9 in 3D Show helices and sheets RCSB PDB PDBe
6NT9 contains 40 α-helices and 38 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2-3 | 2 | 1 |
| β-strand | 7-9 | 3 | 1 |
| β-strand | 14-16 | 3 | 1 |
| β-strand | 21-28 | 8 | 1 |
| β-strand | 34-40 | 7 | 1 |
| α-helix | 50-60 | 11 | |
| β-strand | 67 | 1 | 2 |
| β-strand | 70-75 | 6 | 1 |
| β-strand | 82-86 | 5 | 1 |
| β-strand | 93 | 1 | 2 |
| α-helix | 94-98 | 5 | |
| α-helix | 101-103 | 3 | |
| α-helix | 109-129 | 21 | |
| β-strand | 141-145 | 5 | 2 |
| β-strand | 151-155 | 5 | 2 |
| α-helix | 201-203 | 3 | |
| α-helix | 205-216 | 12 | |
| β-strand | 221-222 | 2 | 3 |
| α-helix | 233-239 | 7 | |
| β-strand | 247-249 | 3 | 3 |
| β-strand | 258-260 | 3 | 3 |
| α-helix | 271-284 | 14 | |
| α-helix | 295-306 | 12 | |
| β-strand | 309-315 | 7 | 4 |
| β-strand | 320-326 | 7 | 4 |
| α-helix | 332-343 | 12 | |
| β-strand | 351-353 | 3 | 4 |
| β-strand | 358-359 | 2 | 4 |
| α-helix | 367-369 | 3 | |
| α-helix | 371-372 | 2 | |
| β-strand | 379-382 | 4 | 4 |
| α-helix | 399-403 | 5 | |
| α-helix | 410-482 | 73 | |
| α-helix | 498-526 | 29 | |
| α-helix | 536-538 | 3 | |
| α-helix | 548-571 | 24 | |
| α-helix | 577-600 | 24 | |
| α-helix | 601-605 | 5 | |
| α-helix | 606-657 | 52 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Serine/threonine-protein kinase TBK1 | A, B | protein | 742 | Homo sapiens | Q9UHD2 (AlphaFold model) |
| Stimulator of interferon genes protein | C, D | protein | 392 | Gallus gallus | E1C7U0 (AlphaFold model) |
>6NT9_1 Serine/threonine-protein kinase TBK1 (chains A, B) MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLK KLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVV GGMNHLRENGIVHRNIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYL HPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITG KPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKCWGFDQFFA ETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLV LEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITG VVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVK VYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQE GTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQIHKFDKQKLYYHATKAMTH FTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQ KMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMD GGLRNVDCLGIHRPDYKDDDDK
>6NT9_2 Stimulator of interferon genes protein (chains C, D) MPQDPSTRSSPARLLIPEPRAGRARHAACVLLAVCFVVLFLSGEPLAPIIRSVCTQLAAL QLGVLLKGCCCLAEEIFHLHSRHHGSLWQVLCSCFPPRWYLALLLVGGSAYLDPPEDNGH SPRLALTLSCLCQLLVLALGLQKLSAVEVSELTESSKKNVAHGLAWSYYIGYLKVVLPRL KECMEELSRTNPMLRAHRDTWKLHILVPLGCDIWDDLEKADSNIQYLADLPETILTRAGI KRRVYKHSLYVIRDKDNKLRPCVLEFASPLQTLCAMSQDDCAAFSREQRLEQARLFYRSL RDILGSSKECAGLYRLIAYEEPAEPESHFLSGLILWHLQQQQREEYMVQEELPLGTSSVE LSLQVSSSDLPQPLRSDCPGIHRPDYKDDDDK
Structural basis of STING binding with and phosphorylation by TBK1. Zhang, C., Shang, G., Gui, X. et al. Nature (2019) 567:394-398. DOI 10.1038/s41586-019-1000-2 · PubMed
Other PDB entries of the same protein (UniProt Q9UHD2 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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