6NUW: Yeast Ctf19 complex

Yeast Ctf19 complex. Determined by electron microscopy at 4.25 Å resolution. Released 3 Apr 2019.

Method: Electron microscopy
Resolution: 4.25 Å
Organisms: Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae S288c
Chains: 13
Atoms: 16,292
Mol. weight: 493.09 kDa
Released: 3 Apr 2019

Explore 6NUW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6NUW contains 104 α-helices and 61 β-strands across 13 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 9 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	3-4	2	1
β-strand	7-10	4	2
α-helix	20-27	8
β-strand	32-38	7	2
β-strand	53-59	7	2
β-strand	61	1	3
β-strand	70	1	3
β-strand	75-77	3	2
β-strand	85-88	4	2
α-helix	95-107	13
β-strand	111-112	2	2
α-helix	118-127	10
β-strand	130	1	4
β-strand	140	1	4
β-strand	146	1	5
β-strand	148-149	2	6
α-helix	154-156	3
β-strand	161	1	7
β-strand	164	1	5
α-helix	167-175	9
α-helix	183	1
α-helix	184-188	5
α-helix	189-196	8
β-strand	198	1	8
β-strand	205-207	3	6
β-strand	209-210	2	1
β-strand	214-215	2	1
β-strand	222-223	2	1
α-helix	230-239	10

Chain C: 8 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	157-172	16
β-strand	175-177	3	9
β-strand	180	1	10
β-strand	201	1	10
β-strand	204-206	3	9
β-strand	209	1	11
β-strand	216	1	11
β-strand	220-222	3	9
β-strand	225	1	10
β-strand	234	1	10
β-strand	238-239	2	9
α-helix	247-251	5
α-helix	253-256	4
α-helix	262-283	22
α-helix	284-289	6
α-helix	290-291	2
β-strand	298-299	2	12
β-strand	305-309	5	12
β-strand	314-318	5	12
β-strand	319	1	13
β-strand	323	1	14
β-strand	324	1	13
α-helix	338-344	7
β-strand	347	1	14
α-helix	348-358	11

Chain D: 6 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	130	1	15
α-helix	134-139	6
α-helix	152-159	8
β-strand	166	1	16
β-strand	178	1	17
β-strand	183	1	16
β-strand	186	1	18
β-strand	192	1	18
β-strand	199	1	17
α-helix	227-231	5
α-helix	237-262	26
β-strand	269	1	19
β-strand	282	1	19
β-strand	300	1	20
β-strand	315	1	20
α-helix	335-344	10
α-helix	350-362	13

Chain E: 15 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	17-26	10
α-helix	32-40	9
α-helix	51-63	13
α-helix	71-78	8
α-helix	88-98	11
β-strand	108-109	2	21
α-helix	128-135	8
β-strand	142	1	21
β-strand	145	1	21
β-strand	153-161	9	21
β-strand	196-205	10	21
β-strand	211-213	3	21
α-helix	221-231	11
β-strand	243	1	21
α-helix	252-255	4
α-helix	271-274	4
α-helix	286-289	4
β-strand	293	1	15
α-helix	302-308	7
α-helix	319-333	15
β-strand	386-392	7	5
β-strand	397-403	7	5
β-strand	404	1	7
α-helix	409-417	9
β-strand	422	1	8
α-helix	424-426	3
α-helix	429-432	4
β-strand	441-442	2	5

Chain F: 7 helices, 0 β-strands

Element	Residues	Length
α-helix	163-189	27
α-helix	198-220	23
α-helix	235-292	58
α-helix	325-329	5
α-helix	347-355	9
α-helix	357-374	18
α-helix	375-380	6

Chain G: 7 helices, 0 β-strands

Element	Residues	Length
α-helix	7-26	20
α-helix	30-33	4
α-helix	43-83	41
α-helix	93-96	4
α-helix	105-112	8
α-helix	141-176	36
α-helix	188-203	16

Chain H: 19 helices, 0 β-strands

Element	Residues	Length
α-helix	44-53	10
α-helix	58-64	7
α-helix	83-87	5
α-helix	106-119	14
α-helix	131-135	5
α-helix	136-141	6
α-helix	142-145	4
α-helix	154-163	10
α-helix	168-182	15
α-helix	190-200	11
α-helix	202-208	7
α-helix	212-231	20
α-helix	298-323	26
α-helix	327-347	21
α-helix	356-373	18
α-helix	381-385	5
α-helix	388-395	8
α-helix	413-429	17
α-helix	438-456	19

Chain I: 9 helices, 0 β-strands

Element	Residues	Length
α-helix	126-149	24
α-helix	171-229	59
α-helix	237-246	10
α-helix	247-251	5
α-helix	252-265	14
α-helix	269-272	4
α-helix	290-298	9
α-helix	299-304	6
α-helix	305-320	16

5 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Inner kinetochore subunit IML3	B	protein	248	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P38265 (AlphaFold model)
Inner kinetochore subunit MCM21	C	protein	371	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q06675 (AlphaFold model)
Inner kinetochore subunit CTF19	D	protein	369	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q02732 (AlphaFold model)
Inner kinetochore subunit CHL4	E	protein	461	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P38907 (AlphaFold model)
Inner kinetochore subunit OKP1	F	protein	406	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P53298
Inner kinetochore subunit NKP1	G	protein	241	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q12493
Inner kinetochore subunit AME1	I	protein	330	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P38313
Inner kinetochore subunit CTF3,Inner kinetochore subunit CTF3	H, Y	protein	773	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae S288c	Q12748
Inner kinetochore subunit NKP2	J	protein	153	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q06162
Inner kinetochore subunit Mcm22	X	protein	126	Saccharomyces cerevisiae S288c
Inner kinetochore subunit Mcm16	M	protein	75	Saccharomyces cerevisiae S288c
Unknown (unassigned)	U	protein	23	Saccharomyces cerevisiae S288c

Sequence of entity 1 (B), FASTA

>6NUW_1 Inner kinetochore subunit IML3 (chains B)
SNAMPYTWKFLGISKQLSLENGIAKLNQLLNLEVDLDIQTIRVPSDPDGGTAADEYIRYE
MRLDISNLDEGTYSKFIFLGNSKMEVPMFLCYCGTDNRNEVVLQWLKAEYGVIMWPIKFE
QKTMIKLADASIVHVTKENIEQITWFSSKLYFEPETQDKNLRQFSIEIPRESCEGLALGY
GNTMHPYNDAIVPYIYNETGMAVERLPLTSVILAGHTKIMRESIVTSTRSLRNRVLAVVL
QSIQFTSE

Sequence of entity 2 (C), FASTA

>6NUW_2 Inner kinetochore subunit MCM21 (chains C)
SNAMSRIDDLQQDIESLLSEINSLEESREKLKAKIKDKRKNEESANPIVQEFEDLFDQFP
QLNNFLFNEHPELEETDDKDISRAQADIPATPIPYEPKKRAKLENEEILPEQEWVLKTQP
MVQHQMFDPGVADLLDTDILTSPSKRKRKLKIDDISTSDRSELEDYIVLENVYRMFGITF
FPLVDPIDLKIKDASGEIFVDREMLGIRLEVFSERTSQFEKPHYVLLKKRIKSNSWFLFK
HTIPSFIDVQGIFDDTNGGLVISHDDAYLFAKRVFLQLVEVQKRRQIFKDLEAKKIIHDL
DLDLESSMVSFFVKDIKVELFVKQNEIVSCSILDDIHDFSQNNKSKWEIALLGSLDDLEL
KLNHSFATIFK

Sequence of entity 3 (D), FASTA

>6NUW_3 Inner kinetochore subunit CTF19 (chains D)
MDFTSDTTNSHDTSNSHLSLEDAVGTHHAGEADVNIDGDEKQQLSLLDDDQVRALKLQEE
KDALLTRRNTLLQEIQTYQNILMKENNSKTKNGDILQNDITQDFLNLISISSSNPNSAIS
DRKRVERINGLTNLQKELVTKYDTLPLLNMNLRLSYLRDHTYPHLQVSVQSRDRVHNDGI
EVLVVNYKFCRNTMNPFEIQFKMFYKFEDSTLLKWEILRISTNVRLKAKQLLATRNFQKC
LLSLYEFDKIKSKKTGIFQNLINLLKRKTRCYLMNNSDSLIVERVIREGRLTTIKLQINF
IITMPGERGKPRNCFLPMSKISIALWKGGERFNQIDLDEICYGLIKEYGVKTGLKEICNV
CLFPDMYAR

Sequence of entity 4 (E), FASTA

>6NUW_4 Inner kinetochore subunit CHL4 (chains E)
SNAMSNELRLEDNYVPTSDTLVVFKQLMKLPVTVLYDLTLSWFAKFGGSFDGDIYLLTET
LDLLIEKGVRRNVIVNRILYVYWPDGLNVFQLAEIDCHLMISKPEKFKWLPSKALRGDGK
PYVVKLQPAKFIENLQTDLAKIYHCHVYMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSI
NKESLYNKLDKFQGKPLISRRPYYVAFPLNSPIIFHSVDKDIYARLVLQSISRTISERET
IIFKPVQKIPVKSIHNIMTLLGPSRFAESMGPWECYASANFERSPLHDYKKHQGLTGKKV
MVREFDDSFLNDDENFYGKEEPEIRRLRLEKNMIKFKGSANGVMDQKYNDLKEFNEHVHN
IRNGKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELC
DKNLINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL

Sequence of entity 5 (F), FASTA

>6NUW_5 Inner kinetochore subunit OKP1 (chains F)
MAADRDNFLQNIENDSINNGQAMDLSPNRSSSESDSSILMNVNDIKTLRLDVAPEAKSTQ
SKKSLFYENSDDAEEGEIEERTNKEEGQYHHKGSKQLRFEVGKESTGKLQSHLSDGSATS
GEGNVRPWEFRKVIQAEYRERLPRNYELKHWKKPSKIMIGSILRLLETNTVSALDSVFEK
YEKEMNQMTHGDNNEVKRIYSKKERLLEIILTKIKKKLRQAKFPSRISERDLDIEYIYSK
RQFIQNRYSQELQNNERLEAILSREQNLLEETRKLCMNLKTNNKKRLTEKLIQKDLHPVL
NKAMEYTYGLESTNGFMHPDGPVTFRNDSHELNLMLNDPIKSTADVRLDKEEVLSLLPSL
KEYTKKSKELKETMGQMISDSHEEEIKEVFVPHHESHQDKTEEDIH

Sequence of entity 6 (G), FASTA

>6NUW_6 Inner kinetochore subunit NKP1 (chains G)
SNAMTDTYNSISNFIENELTALLSSDDYLMDDLAGELPNEVCRLLKAQVIEKRKDAMSRG
KQDLLSKEIYDNESELRASQSQQIMELVGDIPKYSLGSELRNRVEGEPQSTSIERLIEDV
LKLPQMEVADEEEVEVENDLKVLSEYSNLRKDLILKCQALQIGESKLSDILSQTNSINSL
TTSIKEASEDDDISEYFATYNGKLVVALEEMKLLLEEAVKTFGNSPEKREKIKKILSELK
K

Sequence of entity 7 (I), FASTA

>6NUW_7 Inner kinetochore subunit AME1 (chains I)
MDRDTKLAFRLRGSHSRRTDDIDDDVIVFKTPNAVYREENSPIQSPVQPILSSPKLANSF
EFPITTNNVNAQDRHEHGYQPLDAEDYPMIDSENKSLISESPQNVRNDEDLTTRYNFDDI
PIRQLSSSITSVTTIDVLSSLFINLFENDLIPQALKDFNKSDDDQFRKLLYKLDLRLFQT
ISDQMTRDLKDILDINVSNNELCYQLKQVLARKEDLNQQIISVRNEIQELKAGKDWHDLQ
NEQAKLNDKVKLNKRLNDLTSTLLGKYEGDRKIMSQDSEDDSIRDDSNILDIAHFVDLMD
PYNGLLKKINKINENLSNELQPSLHHHHHH

Sequence of entity 8 (H, Y), FASTA

>6NUW_8 Inner kinetochore subunit CTF3,Inner kinetochore subunit CTF3 (chains H, Y)
SNAMSLILDDIILSLTNANERTPPQALKTTLSLLYEKSKQYGLSSPQLQALVRLLCETSI
IDTVTKVYIVENCFLPDGYLTKELLLEIINHLGTPTVFSRYRIQTPPVLQSALCKWLVHV
YFLFPVHSEREHNISSSIWLHLWQFSFLQKWITPLVIWQATTPVDVKPWKLSIIKRCAMH
PGYRDAPGSATLILQRFQCLVGASSQITESIITINCNRKTLKSHRNLKLDAHFLSILKRI
LSRAHPANFPADTVQNTIDMYLSEIHQLGADSIYPLRLQSLPEYVPSDSTVSLWDVTSLE
QLAQNWPQLHIPNDVDYMMKPSLNSNVLLPRKVMSRDSLKHLYSSIILIKNSRDESSSPY
EWCIWQLKRCFAHQIETPQEVIPIIISVSSMDNKLSSRIIQTFCNLKYLKLDELTLKKVC
GGILPLWKPELISGTREFFVKFMASIFMWSTRDGHDNNCTFSETCFYVLQMITNWVLDDK
LIALGLTLLHDMQSLLTLDKIFNNATSNRFSTMAFISSLDILTQLSKQTKSDYAIQYLIV
GPDIMNKVFSSDDPLLLSAACRYLVATKNKLMQYPSTNKFVRMQNQYIMDLTNYLYRNKV
LSSKSLFGVSPDFFKQILENLYIPTADFKNAKFFTITGIPALSYICIIILRRLETAENTK
IKFTSGIINEETFNNFFRVHHDEIGQHGWIKGVNNIHDLRVKILMHLSNTANPYRDIAAF
LFTYLKSLSKYSVQNSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Sequence of entity 9 (J), FASTA

>6NUW_9 Inner kinetochore subunit NKP2 (chains J)
MNSEQLLHNYVSDSLLTTLISFQEFKQQLQSYTSDEQQLQHWYELLQARDARVTSELEAR
IKQFFITLRSRLLRFLESEQLSHSLSLETLIDALYKINDLLQQRLQILDDAIQEKTSELA
EFENMVRSPSAGDNAIPGLLQIIQSYINLLEEN

Sequence of entity 10 (X), FASTA

>6NUW_10 Inner kinetochore subunit Mcm22 (chains X)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXX

Sequence of entity 11 (M), FASTA

>6NUW_11 Inner kinetochore subunit Mcm16 (chains M)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXX

Sequence of entity 12 (U), FASTA

>6NUW_12 Unknown (unassigned) (chains U)
XXXXXXXXXXXXXXXXXXXXXXX

Primary citation

The structure of the Ctf19c/CCAN from budding yeast. Hinshaw, S.M., Harrison, S.C. Elife (2019) 8. DOI 10.7554/eLife.44239 · PubMed

Other PDB entries of the same protein (UniProt P38265 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4JE3 2.28 Å, An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate…
4IT3 2.5 Å, Crystal Structure of Iml3 from S. cerevisiae
4KR1 2.5 Å, Crystal structure of the kinetechore protein Iml3 from budding yeast
10FI 3.3 Å, apo CCAN purified from budding yeast
8OVW 3.4 Å, Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA
8OW0 3.4 Å, Cryo-EM structure of CBF1-CCAN bound topologically to a centromeric CENP-A nucleosome
6QLF 3.45 Å, Structure of inner kinetochore CCAN complex with mask1
6QLE 3.55 Å, Structure of inner kinetochore CCAN complex
8OW1 3.7 Å, Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome.
10DQ 3.9 Å, Cbf1-CCAN-CEN complex
6QLD 4.15 Å, Structure of inner kinetochore CCAN-Cenp-A complex
10JC 6.5 Å, CCAN dimer purified from budding yeast

Browse structure collections

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