6NUW: Yeast Ctf19 complex
Yeast Ctf19 complex. Determined by electron microscopy at 4.25 Å resolution. Released 3 Apr 2019.
- Method
- Electron microscopy
- Resolution
- 4.25 Å
- Organisms
- Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae S288c
- Chains
- 13
- Atoms
- 16,292
- Mol. weight
- 493.09 kDa
- Released
- 3 Apr 2019
Explore 6NUW in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
6NUW contains 104 α-helices and 61 β-strands across 13 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain B: 9 helices, 20 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-4 | 2 | 1 |
| β-strand | 7-10 | 4 | 2 |
| α-helix | 20-27 | 8 | |
| β-strand | 32-38 | 7 | 2 |
| β-strand | 53-59 | 7 | 2 |
| β-strand | 61 | 1 | 3 |
| β-strand | 70 | 1 | 3 |
| β-strand | 75-77 | 3 | 2 |
| β-strand | 85-88 | 4 | 2 |
| α-helix | 95-107 | 13 | |
| β-strand | 111-112 | 2 | 2 |
| α-helix | 118-127 | 10 | |
| β-strand | 130 | 1 | 4 |
| β-strand | 140 | 1 | 4 |
| β-strand | 146 | 1 | 5 |
| β-strand | 148-149 | 2 | 6 |
| α-helix | 154-156 | 3 | |
| β-strand | 161 | 1 | 7 |
| β-strand | 164 | 1 | 5 |
| α-helix | 167-175 | 9 | |
| α-helix | 183 | 1 | |
| α-helix | 184-188 | 5 | |
| α-helix | 189-196 | 8 | |
| β-strand | 198 | 1 | 8 |
| β-strand | 205-207 | 3 | 6 |
| β-strand | 209-210 | 2 | 1 |
| β-strand | 214-215 | 2 | 1 |
| β-strand | 222-223 | 2 | 1 |
| α-helix | 230-239 | 10 | |
Chain C: 8 helices, 17 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 157-172 | 16 | |
| β-strand | 175-177 | 3 | 9 |
| β-strand | 180 | 1 | 10 |
| β-strand | 201 | 1 | 10 |
| β-strand | 204-206 | 3 | 9 |
| β-strand | 209 | 1 | 11 |
| β-strand | 216 | 1 | 11 |
| β-strand | 220-222 | 3 | 9 |
| β-strand | 225 | 1 | 10 |
| β-strand | 234 | 1 | 10 |
| β-strand | 238-239 | 2 | 9 |
| α-helix | 247-251 | 5 | |
| α-helix | 253-256 | 4 | |
| α-helix | 262-283 | 22 | |
| α-helix | 284-289 | 6 | |
| α-helix | 290-291 | 2 | |
| β-strand | 298-299 | 2 | 12 |
| β-strand | 305-309 | 5 | 12 |
| β-strand | 314-318 | 5 | 12 |
| β-strand | 319 | 1 | 13 |
| β-strand | 323 | 1 | 14 |
| β-strand | 324 | 1 | 13 |
| α-helix | 338-344 | 7 | |
| β-strand | 347 | 1 | 14 |
| α-helix | 348-358 | 11 | |
Chain D: 6 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 130 | 1 | 15 |
| α-helix | 134-139 | 6 | |
| α-helix | 152-159 | 8 | |
| β-strand | 166 | 1 | 16 |
| β-strand | 178 | 1 | 17 |
| β-strand | 183 | 1 | 16 |
| β-strand | 186 | 1 | 18 |
| β-strand | 192 | 1 | 18 |
| β-strand | 199 | 1 | 17 |
| α-helix | 227-231 | 5 | |
| α-helix | 237-262 | 26 | |
| β-strand | 269 | 1 | 19 |
| β-strand | 282 | 1 | 19 |
| β-strand | 300 | 1 | 20 |
| β-strand | 315 | 1 | 20 |
| α-helix | 335-344 | 10 | |
| α-helix | 350-362 | 13 | |
Chain E: 15 helices, 13 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 17-26 | 10 | |
| α-helix | 32-40 | 9 | |
| α-helix | 51-63 | 13 | |
| α-helix | 71-78 | 8 | |
| α-helix | 88-98 | 11 | |
| β-strand | 108-109 | 2 | 21 |
| α-helix | 128-135 | 8 | |
| β-strand | 142 | 1 | 21 |
| β-strand | 145 | 1 | 21 |
| β-strand | 153-161 | 9 | 21 |
| β-strand | 196-205 | 10 | 21 |
| β-strand | 211-213 | 3 | 21 |
| α-helix | 221-231 | 11 | |
| β-strand | 243 | 1 | 21 |
| α-helix | 252-255 | 4 | |
| α-helix | 271-274 | 4 | |
| α-helix | 286-289 | 4 | |
| β-strand | 293 | 1 | 15 |
| α-helix | 302-308 | 7 | |
| α-helix | 319-333 | 15 | |
| β-strand | 386-392 | 7 | 5 |
| β-strand | 397-403 | 7 | 5 |
| β-strand | 404 | 1 | 7 |
| α-helix | 409-417 | 9 | |
| β-strand | 422 | 1 | 8 |
| α-helix | 424-426 | 3 | |
| α-helix | 429-432 | 4 | |
| β-strand | 441-442 | 2 | 5 |
Chain F: 7 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 163-189 | 27 | |
| α-helix | 198-220 | 23 | |
| α-helix | 235-292 | 58 | |
| α-helix | 325-329 | 5 | |
| α-helix | 347-355 | 9 | |
| α-helix | 357-374 | 18 | |
| α-helix | 375-380 | 6 | |
Chain G: 7 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-26 | 20 | |
| α-helix | 30-33 | 4 | |
| α-helix | 43-83 | 41 | |
| α-helix | 93-96 | 4 | |
| α-helix | 105-112 | 8 | |
| α-helix | 141-176 | 36 | |
| α-helix | 188-203 | 16 | |
Chain H: 19 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 44-53 | 10 | |
| α-helix | 58-64 | 7 | |
| α-helix | 83-87 | 5 | |
| α-helix | 106-119 | 14 | |
| α-helix | 131-135 | 5 | |
| α-helix | 136-141 | 6 | |
| α-helix | 142-145 | 4 | |
| α-helix | 154-163 | 10 | |
| α-helix | 168-182 | 15 | |
| α-helix | 190-200 | 11 | |
| α-helix | 202-208 | 7 | |
| α-helix | 212-231 | 20 | |
| α-helix | 298-323 | 26 | |
| α-helix | 327-347 | 21 | |
| α-helix | 356-373 | 18 | |
| α-helix | 381-385 | 5 | |
| α-helix | 388-395 | 8 | |
| α-helix | 413-429 | 17 | |
| α-helix | 438-456 | 19 | |
Chain I: 9 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 126-149 | 24 | |
| α-helix | 171-229 | 59 | |
| α-helix | 237-246 | 10 | |
| α-helix | 247-251 | 5 | |
| α-helix | 252-265 | 14 | |
| α-helix | 269-272 | 4 | |
| α-helix | 290-298 | 9 | |
| α-helix | 299-304 | 6 | |
| α-helix | 305-320 | 16 | |
5 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Inner kinetochore subunit IML3 | B | protein | 248 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | P38265 (AlphaFold model) |
| Inner kinetochore subunit MCM21 | C | protein | 371 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Q06675 (AlphaFold model) |
| Inner kinetochore subunit CTF19 | D | protein | 369 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Q02732 (AlphaFold model) |
| Inner kinetochore subunit CHL4 | E | protein | 461 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | P38907 (AlphaFold model) |
| Inner kinetochore subunit OKP1 | F | protein | 406 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | P53298 |
| Inner kinetochore subunit NKP1 | G | protein | 241 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Q12493 |
| Inner kinetochore subunit AME1 | I | protein | 330 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | P38313 |
| Inner kinetochore subunit CTF3,Inner kinetochore subunit CTF3 | H, Y | protein | 773 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae S288c | Q12748 |
| Inner kinetochore subunit NKP2 | J | protein | 153 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Q06162 |
| Inner kinetochore subunit Mcm22 | X | protein | 126 | Saccharomyces cerevisiae S288c | |
| Inner kinetochore subunit Mcm16 | M | protein | 75 | Saccharomyces cerevisiae S288c | |
| Unknown (unassigned) | U | protein | 23 | Saccharomyces cerevisiae S288c | |
Sequence of entity 1 (B), FASTA
>6NUW_1 Inner kinetochore subunit IML3 (chains B)
SNAMPYTWKFLGISKQLSLENGIAKLNQLLNLEVDLDIQTIRVPSDPDGGTAADEYIRYE
MRLDISNLDEGTYSKFIFLGNSKMEVPMFLCYCGTDNRNEVVLQWLKAEYGVIMWPIKFE
QKTMIKLADASIVHVTKENIEQITWFSSKLYFEPETQDKNLRQFSIEIPRESCEGLALGY
GNTMHPYNDAIVPYIYNETGMAVERLPLTSVILAGHTKIMRESIVTSTRSLRNRVLAVVL
QSIQFTSE
Sequence of entity 2 (C), FASTA
>6NUW_2 Inner kinetochore subunit MCM21 (chains C)
SNAMSRIDDLQQDIESLLSEINSLEESREKLKAKIKDKRKNEESANPIVQEFEDLFDQFP
QLNNFLFNEHPELEETDDKDISRAQADIPATPIPYEPKKRAKLENEEILPEQEWVLKTQP
MVQHQMFDPGVADLLDTDILTSPSKRKRKLKIDDISTSDRSELEDYIVLENVYRMFGITF
FPLVDPIDLKIKDASGEIFVDREMLGIRLEVFSERTSQFEKPHYVLLKKRIKSNSWFLFK
HTIPSFIDVQGIFDDTNGGLVISHDDAYLFAKRVFLQLVEVQKRRQIFKDLEAKKIIHDL
DLDLESSMVSFFVKDIKVELFVKQNEIVSCSILDDIHDFSQNNKSKWEIALLGSLDDLEL
KLNHSFATIFK
Sequence of entity 3 (D), FASTA
>6NUW_3 Inner kinetochore subunit CTF19 (chains D)
MDFTSDTTNSHDTSNSHLSLEDAVGTHHAGEADVNIDGDEKQQLSLLDDDQVRALKLQEE
KDALLTRRNTLLQEIQTYQNILMKENNSKTKNGDILQNDITQDFLNLISISSSNPNSAIS
DRKRVERINGLTNLQKELVTKYDTLPLLNMNLRLSYLRDHTYPHLQVSVQSRDRVHNDGI
EVLVVNYKFCRNTMNPFEIQFKMFYKFEDSTLLKWEILRISTNVRLKAKQLLATRNFQKC
LLSLYEFDKIKSKKTGIFQNLINLLKRKTRCYLMNNSDSLIVERVIREGRLTTIKLQINF
IITMPGERGKPRNCFLPMSKISIALWKGGERFNQIDLDEICYGLIKEYGVKTGLKEICNV
CLFPDMYAR
Sequence of entity 4 (E), FASTA
>6NUW_4 Inner kinetochore subunit CHL4 (chains E)
SNAMSNELRLEDNYVPTSDTLVVFKQLMKLPVTVLYDLTLSWFAKFGGSFDGDIYLLTET
LDLLIEKGVRRNVIVNRILYVYWPDGLNVFQLAEIDCHLMISKPEKFKWLPSKALRGDGK
PYVVKLQPAKFIENLQTDLAKIYHCHVYMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSI
NKESLYNKLDKFQGKPLISRRPYYVAFPLNSPIIFHSVDKDIYARLVLQSISRTISERET
IIFKPVQKIPVKSIHNIMTLLGPSRFAESMGPWECYASANFERSPLHDYKKHQGLTGKKV
MVREFDDSFLNDDENFYGKEEPEIRRLRLEKNMIKFKGSANGVMDQKYNDLKEFNEHVHN
IRNGKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELC
DKNLINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL
Sequence of entity 5 (F), FASTA
>6NUW_5 Inner kinetochore subunit OKP1 (chains F)
MAADRDNFLQNIENDSINNGQAMDLSPNRSSSESDSSILMNVNDIKTLRLDVAPEAKSTQ
SKKSLFYENSDDAEEGEIEERTNKEEGQYHHKGSKQLRFEVGKESTGKLQSHLSDGSATS
GEGNVRPWEFRKVIQAEYRERLPRNYELKHWKKPSKIMIGSILRLLETNTVSALDSVFEK
YEKEMNQMTHGDNNEVKRIYSKKERLLEIILTKIKKKLRQAKFPSRISERDLDIEYIYSK
RQFIQNRYSQELQNNERLEAILSREQNLLEETRKLCMNLKTNNKKRLTEKLIQKDLHPVL
NKAMEYTYGLESTNGFMHPDGPVTFRNDSHELNLMLNDPIKSTADVRLDKEEVLSLLPSL
KEYTKKSKELKETMGQMISDSHEEEIKEVFVPHHESHQDKTEEDIH
Sequence of entity 6 (G), FASTA
>6NUW_6 Inner kinetochore subunit NKP1 (chains G)
SNAMTDTYNSISNFIENELTALLSSDDYLMDDLAGELPNEVCRLLKAQVIEKRKDAMSRG
KQDLLSKEIYDNESELRASQSQQIMELVGDIPKYSLGSELRNRVEGEPQSTSIERLIEDV
LKLPQMEVADEEEVEVENDLKVLSEYSNLRKDLILKCQALQIGESKLSDILSQTNSINSL
TTSIKEASEDDDISEYFATYNGKLVVALEEMKLLLEEAVKTFGNSPEKREKIKKILSELK
K
Sequence of entity 7 (I), FASTA
>6NUW_7 Inner kinetochore subunit AME1 (chains I)
MDRDTKLAFRLRGSHSRRTDDIDDDVIVFKTPNAVYREENSPIQSPVQPILSSPKLANSF
EFPITTNNVNAQDRHEHGYQPLDAEDYPMIDSENKSLISESPQNVRNDEDLTTRYNFDDI
PIRQLSSSITSVTTIDVLSSLFINLFENDLIPQALKDFNKSDDDQFRKLLYKLDLRLFQT
ISDQMTRDLKDILDINVSNNELCYQLKQVLARKEDLNQQIISVRNEIQELKAGKDWHDLQ
NEQAKLNDKVKLNKRLNDLTSTLLGKYEGDRKIMSQDSEDDSIRDDSNILDIAHFVDLMD
PYNGLLKKINKINENLSNELQPSLHHHHHH
Sequence of entity 8 (H, Y), FASTA
>6NUW_8 Inner kinetochore subunit CTF3,Inner kinetochore subunit CTF3 (chains H, Y)
SNAMSLILDDIILSLTNANERTPPQALKTTLSLLYEKSKQYGLSSPQLQALVRLLCETSI
IDTVTKVYIVENCFLPDGYLTKELLLEIINHLGTPTVFSRYRIQTPPVLQSALCKWLVHV
YFLFPVHSEREHNISSSIWLHLWQFSFLQKWITPLVIWQATTPVDVKPWKLSIIKRCAMH
PGYRDAPGSATLILQRFQCLVGASSQITESIITINCNRKTLKSHRNLKLDAHFLSILKRI
LSRAHPANFPADTVQNTIDMYLSEIHQLGADSIYPLRLQSLPEYVPSDSTVSLWDVTSLE
QLAQNWPQLHIPNDVDYMMKPSLNSNVLLPRKVMSRDSLKHLYSSIILIKNSRDESSSPY
EWCIWQLKRCFAHQIETPQEVIPIIISVSSMDNKLSSRIIQTFCNLKYLKLDELTLKKVC
GGILPLWKPELISGTREFFVKFMASIFMWSTRDGHDNNCTFSETCFYVLQMITNWVLDDK
LIALGLTLLHDMQSLLTLDKIFNNATSNRFSTMAFISSLDILTQLSKQTKSDYAIQYLIV
GPDIMNKVFSSDDPLLLSAACRYLVATKNKLMQYPSTNKFVRMQNQYIMDLTNYLYRNKV
LSSKSLFGVSPDFFKQILENLYIPTADFKNAKFFTITGIPALSYICIIILRRLETAENTK
IKFTSGIINEETFNNFFRVHHDEIGQHGWIKGVNNIHDLRVKILMHLSNTANPYRDIAAF
LFTYLKSLSKYSVQNSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sequence of entity 9 (J), FASTA
>6NUW_9 Inner kinetochore subunit NKP2 (chains J)
MNSEQLLHNYVSDSLLTTLISFQEFKQQLQSYTSDEQQLQHWYELLQARDARVTSELEAR
IKQFFITLRSRLLRFLESEQLSHSLSLETLIDALYKINDLLQQRLQILDDAIQEKTSELA
EFENMVRSPSAGDNAIPGLLQIIQSYINLLEEN
Sequence of entity 10 (X), FASTA
>6NUW_10 Inner kinetochore subunit Mcm22 (chains X)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXX
Sequence of entity 11 (M), FASTA
>6NUW_11 Inner kinetochore subunit Mcm16 (chains M)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXX
Sequence of entity 12 (U), FASTA
>6NUW_12 Unknown (unassigned) (chains U)
XXXXXXXXXXXXXXXXXXXXXXX
Primary citation
The structure of the Ctf19c/CCAN from budding yeast. Hinshaw, S.M., Harrison, S.C. Elife (2019) 8. DOI 10.7554/eLife.44239 · PubMed
Other PDB entries of the same protein (UniProt P38265 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 4JE3 2.28 Å, An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate…
- 4IT3 2.5 Å, Crystal Structure of Iml3 from S. cerevisiae
- 4KR1 2.5 Å, Crystal structure of the kinetechore protein Iml3 from budding yeast
- 10FI 3.3 Å, apo CCAN purified from budding yeast
- 8OVW 3.4 Å, Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA
- 8OW0 3.4 Å, Cryo-EM structure of CBF1-CCAN bound topologically to a centromeric CENP-A nucleosome
- 6QLF 3.45 Å, Structure of inner kinetochore CCAN complex with mask1
- 6QLE 3.55 Å, Structure of inner kinetochore CCAN complex
- 8OW1 3.7 Å, Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome.
- 10DQ 3.9 Å, Cbf1-CCAN-CEN complex
- 6QLD 4.15 Å, Structure of inner kinetochore CCAN-Cenp-A complex
- 10JC 6.5 Å, CCAN dimer purified from budding yeast
Browse structure collections
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