6OX4: A SETD3 Mutant

A SETD3 Mutant (N255A) in Complex with an Actin Peptide. Determined by X-ray diffraction at 2.29 Å resolution. Released 21 Aug 2019.

Method: X-ray diffraction
Resolution: 2.29 Å
Organism: Homo sapiens
Chains: 4
Atoms: 8,630
Mol. weight: 142.15 kDa
Ligands: SAH
Released: 21 Aug 2019

Explore 6OX4 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6OX4 contains 60 α-helices and 33 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 29 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	21-36	16
α-helix	38-39	2
α-helix	42-44	3
α-helix	45-61	17
α-helix	74-77	4
α-helix	78-87	10
β-strand	95-100	6	2
β-strand	104-109	6	2
β-strand	113	1	3
β-strand	118-123	6	1
α-helix	124-126	3
β-strand	128-129	2	4
α-helix	130-134	5
α-helix	139-142	4
α-helix	146-150	5
α-helix	152-164	13
α-helix	172-175	4
α-helix	185-187	3
α-helix	190-194	5
α-helix	201-225	25
α-helix	227-229	3
α-helix	233-235	3
α-helix	240-253	14
β-strand	255-258	4	4
β-strand	265-269	5	4
α-helix	273-275	3
β-strand	277-278	2	5
β-strand	285-288	4	1
β-strand	293-297	5	1
β-strand	302	1	3
β-strand	307-308	2	2
β-strand	309-310	2	5
α-helix	317-319	3
α-helix	320-324	5
β-strand	335-341	7	6
α-helix	349-358	10
β-strand	364-370	7	6
α-helix	378-387	10
α-helix	391-396	6
α-helix	403-408	6
α-helix	419-437	19
α-helix	444-453	10
α-helix	458-493	36
α-helix	496-499	4

Chain B: 30 helices, 16 β-strands

Element	Residues	Length	Sheet
α-helix	21-36	16
α-helix	38-39	2
α-helix	42-44	3
α-helix	45-61	17
α-helix	74-77	4
α-helix	78-87	10
β-strand	95-100	6	8
β-strand	104-109	6	8
β-strand	113	1	9
β-strand	118-123	6	7
α-helix	124-126	3
β-strand	128-129	2	10
α-helix	130-134	5
α-helix	139-142	4
α-helix	146-150	5
α-helix	152-164	13
α-helix	172-175	4
α-helix	185-187	3
α-helix	190-194	5
α-helix	202-225	24
α-helix	227-229	3
α-helix	233-235	3
α-helix	240-253	14
β-strand	255-258	4	10
β-strand	265-269	5	10
α-helix	273-275	3
α-helix	276	1
β-strand	277-278	2	11
β-strand	285-288	4	7
β-strand	293-297	5	7
β-strand	302	1	9
β-strand	307-308	2	8
β-strand	309-310	2	11
α-helix	317-319	3
α-helix	320-324	5
β-strand	335-341	7	12
α-helix	349-358	10
β-strand	364-370	7	12
β-strand	376	1	12
α-helix	378-387	10
α-helix	391-396	6
α-helix	403-408	6
α-helix	419-437	19
α-helix	444-453	10
α-helix	458-493	36
α-helix	496-499	4

Chain Y: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	70	1	1

Chain Z: 1 helix, 1 β-strand

Element	Residues	Length	Sheet
β-strand	70	1	7
α-helix	71-72	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin Peptide	Y, Z	protein	15	Homo sapiens	P60709 (AlphaFold model)
Actin-histidine N-methyltransferase	A, B	protein	599	Homo sapiens	Q86TU7 (AlphaFold model)

Sequence of entity 1 (Y, Z), FASTA

>6OX4_1 Actin Peptide (chains Y, Z)
TLKYPIEHGIVTNWD

Sequence of entity 2 (A, B), FASTA

>6OX4_2 Actin-histidine N-methyltransferase (chains A, B)
GPLGSMGKKSRVKTQKSGTGATATVSPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRT
LVEKIRKKQKGLSVTFDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIK
AEELFLWVPRKLLMTVESAKNSVLGPLYSQDRILQAMGNIALAFHLLCERASPNSFWQPY
IQTLPSEYDTPLYFEEDEVRYLQSTQAIHDVFSQYKNTARQYAYFYKVIQTHPHANKLPL
KDSFTYEDYRWAVSSVMTRQAQIPTEDGSRVTLALIPLWDMCNHTNGLITTGYNLEDDRC
ECVALQDFRAGEQIYIFYGTRSNAEFVIHSGFFFDNNSHDRVKIKLGVSKSDRLYAMKAE
VLARAGIPTSSVFALHFTEPPISAQLLAFLRVFCMTEEELKEHLLGDSAIDRIFTLGNSE
FPVSWDNEVKLWTFLEDRASLLLKTYKTTIEEDKSVLKNHDLSVRAKMAIKLRLGEKEIL
EKAVKSAAVNREYYRQQMEEKAPLPKYEESNLGLLESSVGDSRLPLVLRNLEEEAGVQDA
LNIREAISKAKATENGLVNGENSIPNGTRSENESLNQESKRAVEDAKGSSSDSTAGVKE

Ligands and cofactors

ID	Name	Formula	Copies
SAH	S-adenosyl-L-homocysteine	C14 H20 N6 O5 S	2

Water and common crystallization additives (ACT, GOL, EDO) are not listed.

Primary citation

Structural basis for the target specificity of actin histidine methyltransferase SETD3. Dai, S., Horton, J.R., Woodcock, C.B. et al. Nat Commun (2019) 10:3541-3541. DOI 10.1038/s41467-019-11554-6 · PubMed

Other PDB entries of the same protein (UniProt P60709 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6MBK 1.69 Å, SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, First…
6OX0 1.75 Å, SETD3 in Complex with an Actin Peptide with Sinefungin Replacing SAH as Cofactor
6MBJ 1.78 Å, SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, P21…
6OX3 1.78 Å, SETD3 in Complex with an Actin Peptide with His73 Replaced with Lysine
7W28 1.79 Å, Crystal Structure of SETD3-SAH in complex with betaA-4PyrAla73 peptide
6OX1 1.95 Å, SETD3 in Complex with an Actin Peptide with Target Histidine Partially Methylated
6ICT 1.95 Å, Structure of SETD3 bound to SAH and methylated actin
6OX2 2.09 Å, SETD3in Complex with an Actin Peptide with the Target Histidine Fully Methylated
6OX5 2.1 Å, A SETD3 Mutant (N255A) in Complex with an Actin Peptide with His73 Replaced with Lysine
6ICV 2.15 Å, Structure of SETD3 bound to SAH and unmodified actin
9QEW 2.18 Å, Cryo-EM structure of the undecorated actin filament in the ADP-Pi state.
6MBL 2.2 Å, SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, Second…

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