6P9X: PDB entry 6P9X

CRF1 Receptor Gs GPCR protein complex with CRF1 peptide. Determined by electron microscopy at 2.91 Å resolution. Released 5 Feb 2020.

Method: Electron microscopy
Resolution: 2.91 Å
Organisms: Homo sapiens, Lama glama
Chains: 6
Atoms: 8,177
Mol. weight: 161.39 kDa
Released: 5 Feb 2020

Explore 6P9X in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6P9X contains 36 α-helices and 51 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	14-39	26
β-strand	41-46	6	1
β-strand	208-210	3	1
β-strand	213-214	2	2
β-strand	217-218	2	2
β-strand	219-223	5	1
β-strand	243-248	6	1
α-helix	265-278	14
β-strand	286-291	6	1
α-helix	313-315	3
α-helix	326-327	2
α-helix	332-349	18
β-strand	359-363	5	1
α-helix	372-390	19

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	6-24	19
α-helix	30-33	4
α-helix	38-41	4
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-153	8	6
β-strand	156-161	6	6
β-strand	165-169	5	6
β-strand	176-181	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	303-308	6	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	10-21	12
α-helix	30-43	14
α-helix	54-55	2

Chain N: 2 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	10-11	2	11
β-strand	18-25	8	10
α-helix	29-31	3
β-strand	34-39	6	11
β-strand	45-51	7	11
β-strand	59-60	2	11
β-strand	68-73	6	10
β-strand	78-83	6	10
α-helix	88-90	3
β-strand	92-95	4	11
β-strand	97	1	12
β-strand	98	1	11
β-strand	118	1	12
β-strand	122-125	4	11

Chain P: 3 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	3-5	3
β-strand	6	1	13
α-helix	7-9	3
α-helix	10-31	22

Chain R: 19 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	114-143	30
α-helix	150-174	25
α-helix	178-183	6
α-helix	186-206	21
α-helix	210-214	5
α-helix	215-219	5
α-helix	228-232	5
α-helix	233-237	5
α-helix	239-252	14
α-helix	257-259	3
α-helix	261-263	3
α-helix	270-301	32
α-helix	305-318	14
α-helix	320-323	4
α-helix	326-329	4
β-strand	332	1	13
α-helix	338-353	16
α-helix	355-360	6
α-helix	361-365	5
α-helix	368-379	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	138	Lama glama
Corticotropin-releasing factor receptor 1	R	protein	427	Homo sapiens	P34998 (AlphaFold model)
Corticoliberin	P	protein	41	Homo sapiens	P06850

Sequence of entity 1 (A), FASTA

>6P9X_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQEALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>6P9X_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>6P9X_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>6P9X_4 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 5 (R), FASTA

>6P9X_5 Corticotropin-releasing factor receptor 1 (chains R)
DYKDDDDLEVLFQGPASLQDQHCESLSLASNISGLQCNASVDLIGTCWPRSPAGQLVVRP
CPAFFYGVRYNTTNNGYRECLANGSWAARVNYSECQEILNEEKKSKVHYHVAVIINYLGH
CISLVALLVAFVLFLRLRSIRCLRNIIHWNLISAFILRNATWFVVQLTMSPEVHQSNVGW
CRLVTAAYNYFHVTNFFWMFGEGCYLHTAIVLTYTTDRLRKWMFICIGWGVPFPIIVAWA
IGKLYYDNEKCWFGKRPGVYTDYIYQGPMILVLLINFIFLFNIVRILMTKLRASTTSETI
QYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSFLESFQGFFVSVFYCFLN
SEVRSAIRKRWHRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAVPAGLEVLFQGPH
HHHHHHH

Sequence of entity 6 (P), FASTA

>6P9X_6 Corticoliberin (chains P)
SEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEII

Primary citation

Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation. Liang, Y.L., Belousoff, M.J., Zhao, P. et al. Mol Cell (2020) 77:656-668.e5. DOI 10.1016/j.molcel.2020.01.012 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

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