6P9Y: PAC1 GPCR Receptor complex

PAC1 GPCR Receptor complex. Determined by electron microscopy at 3.01 Å resolution. Released 5 Feb 2020.

Method: Electron microscopy
Resolution: 3.01 Å
Organisms: Homo sapiens, Lama glama
Chains: 6
Atoms: 7,994
Mol. weight: 167.22 kDa
Released: 5 Feb 2020

Explore 6P9Y in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6P9Y contains 34 α-helices and 46 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	14-39	26
β-strand	41-46	6	1
β-strand	208-214	7	1
β-strand	217-223	7	1
β-strand	243-248	6	1
α-helix	265-277	13
α-helix	281-284	4
β-strand	286-291	6	1
α-helix	313-316	4
α-helix	326-327	2
α-helix	332-351	20
β-strand	359-361	3	1
α-helix	372-390	19

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	157-161	5	5
β-strand	166-169	4	5
β-strand	176-180	5	5
β-strand	187-192	6	6
β-strand	199-203	5	6
β-strand	208-211	4	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	241-245	5	7
β-strand	249-253	5	7
β-strand	260-265	6	7
α-helix	272	1
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	295-298	4	8
β-strand	304-307	4	8
β-strand	317-320	4	2
β-strand	327-330	4	2
β-strand	336-339	4	2

Chain G: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	10-22	13
α-helix	30-33	4
α-helix	38-41	4
α-helix	45-47	3
α-helix	53-55	3

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	9
β-strand	10-11	2	10
β-strand	17-20	4	9
β-strand	22-25	4	9
α-helix	29-31	3
β-strand	34-39	6	10
β-strand	45-51	7	10
β-strand	58-60	3	10
α-helix	62-64	3
β-strand	68-73	6	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-95	4	10
β-strand	98	1	10
β-strand	122-125	4	10

Chain P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	3-26	24

Chain R: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	150-163	14
α-helix	166-177	12
α-helix	186-210	25
α-helix	224-255	32
α-helix	265-273	9
α-helix	276-288	13
α-helix	303-336	34
α-helix	347-362	16
α-helix	366-369	4
α-helix	379-382	4
α-helix	383-387	5
α-helix	392-396	5
α-helix	397-401	5
α-helix	405-416	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	138	Lama glama
Pituitary adenylate cyclase-activating polypeptide	P	protein	39	Homo sapiens	P18509 (AlphaFold model)
Pituitary adenylate cyclase-activating polypeptide type I receptor	R	protein	483	Homo sapiens	P41586

Sequence of entity 1 (A), FASTA

>6P9Y_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQEALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>6P9Y_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>6P9Y_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>6P9Y_4 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 5 (P), FASTA

>6P9Y_5 Pituitary adenylate cyclase-activating polypeptide (chains P)
HSDGIFTDSYSRYRKQMAVKKYLAAVLGKRYKQRVKNKX

Sequence of entity 6 (R), FASTA

>6P9Y_6 Pituitary adenylate cyclase-activating polypeptide type I receptor (chains R)
DYKDDDDLEVLFQGPAMHSDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWDNITCW
KPAHVGEMVLVSCPELFRIFNPDQVWETETIGESDFGDSNSLDLSDMGVVSRNCTEDGWS
EPFPHYFDACGFDEYESETGDQDYYYLSVKALYTVGYSTSLVTLTTAMVILCRFRKLHCT
RNFIHMNLFVSFMLRAISVFIKDWILYAEQDSNHCFISTVECKAVMVFFHYCVVSNYFWL
FIEGLYLFTLLVETFFPERRYFYWYTIIGWGTPTVCVTVWATLRLYFDDTGCWDMNDSTA
LWWVIKGPVVGSIMVNFVLFIGIIVILVQKLQSPDMGGNESSIYLRLARSTLLLIPLFGI
HYTVFAFSPENVSKRERLVFELGLGSFQGFVVAVLYCFLNGEVQAEIKRKWRSWKVNRYF
AVDFKHRHPSLASSGVNGGTQLSILSKSSSQIRMSGLPADNLATPAGLEVLFQGPHHHHH
HHH

Primary citation

Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation. Liang, Y.L., Belousoff, M.J., Zhao, P. et al. Mol Cell (2020) 77:656-668.e5. DOI 10.1016/j.molcel.2020.01.012 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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