6QDV: Human post-catalytic P complex spliceosome

Human post-catalytic P complex spliceosome. Determined by electron microscopy at 3.3 Å resolution. Released 20 Feb 2019.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Homo sapiens, Human adenovirus 2
Chains: 54
Atoms: 121,152
Mol. weight: 2322.14 kDa
Ligands: MG, ATP, GTP, ZN
Released: 20 Feb 2019

Explore 6QDV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6QDV contains 661 α-helices and 545 β-strands across 49 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 7: 21 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	40-42	3
α-helix	47-56	10
α-helix	63-74	12
β-strand	78-81	4	12
α-helix	88-98	11
β-strand	109-112	4	12
α-helix	116-129	14
β-strand	137-140	4	12
α-helix	146-155	10
β-strand	159-162	4	12
α-helix	164-172	9
β-strand	183-187	5	12
α-helix	189-192	4
α-helix	198-205	8
β-strand	213-218	6	12
α-helix	223-228	6
α-helix	229-231	3
β-strand	237-239	3	12
α-helix	243-245	3
β-strand	251-258	8	13
α-helix	262-272	11
α-helix	273-275	3
β-strand	279-283	5	13
α-helix	287-298	12
β-strand	305-307	3	13
α-helix	313-325	13
β-strand	330-333	4	13
α-helix	335-337	3
β-strand	346-351	6	13
α-helix	358-365	8
α-helix	370-372	3
β-strand	375-382	8	13
α-helix	383-385	3
α-helix	386-396	11
β-strand	400-402	3	13

Chain 8: 4 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	66	1	14
β-strand	73-78	6	15
α-helix	86-93	8
α-helix	94-96	3
β-strand	99-106	8	15
α-helix	107	1
β-strand	113-121	9	15
α-helix	124-134	11
β-strand	138	1	16
β-strand	143	1	16
β-strand	145-148	4	15
β-strand	150-151	2	14

Chain 9: 7 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	7-17	11	14
β-strand	20-28	9	14
β-strand	33-39	7	14
α-helix	42-44	3
β-strand	48-54	7	14
α-helix	56-69	14
α-helix	71-73	3
α-helix	80-82	3
β-strand	87-94	8	14
β-strand	97-103	7	14
α-helix	109-114	6
α-helix	118-143	26
α-helix	144-146	3

Chain A: 104 helices, 67 β-strands

Element	Residues	Length	Sheet
α-helix	28-44	17
α-helix	54-57	4
α-helix	63-72	10
α-helix	84-90	7
α-helix	91-94	4
α-helix	95-103	9
α-helix	106-108	3
β-strand	115-121	7	20
β-strand	127-129	3	20
β-strand	134-135	2	21
α-helix	137-157	21
α-helix	174-177	4
α-helix	178-181	4
α-helix	186-187	2
α-helix	201-203	3
β-strand	229	1	22
α-helix	232-241	10
α-helix	260-268	9
α-helix	310-315	6
α-helix	357-358	2
β-strand	379	1	23
α-helix	398-405	8
β-strand	415	1	22
α-helix	418	1
β-strand	419-420	2	21
α-helix	428-431	4
α-helix	434-436	3
α-helix	441-459	19
α-helix	473-477	5
β-strand	482-488	7	20
α-helix	489-510	22
β-strand	516-518	3	24
β-strand	524-526	3	24
α-helix	532-537	6
α-helix	544-565	22
α-helix	571-583	13
α-helix	585-588	4
α-helix	591-593	3
α-helix	599-616	18
α-helix	631-659	29
α-helix	677-692	16
α-helix	703-705	3
α-helix	706-721	16
β-strand	726	1	25
α-helix	733-763	31
α-helix	769-796	28
α-helix	803-819	17
α-helix	823-825	3
α-helix	835-848	14
α-helix	863-872	10
α-helix	874-887	14
β-strand	894-900	7	26
β-strand	905-910	6	26
α-helix	913-931	19
β-strand	940	1	27
α-helix	948-960	13
β-strand	973-980	8	27
α-helix	985-987	3
α-helix	990-998	9
α-helix	1003-1012	10
β-strand	1016-1018	3	26
β-strand	1023-1025	3	26
α-helix	1037-1061	25
α-helix	1080-1082	3
β-strand	1085-1090	6	27
β-strand	1094-1101	8	27
α-helix	1103-1116	14
α-helix	1118-1119	2
α-helix	1123-1126	4
α-helix	1136-1138	3
α-helix	1144-1158	15
β-strand	1174-1178	5	27
β-strand	1185-1189	5	28
β-strand	1192-1198	7	28
β-strand	1213-1217	5	28
β-strand	1224-1232	9	28
α-helix	1234-1249	16
α-helix	1256-1271	16
α-helix	1277-1280	4
α-helix	1282-1302	21
α-helix	1314-1317	4
α-helix	1320-1322	3
β-strand	1329	1	29
β-strand	1334-1337	4	30
α-helix	1340-1343	4
β-strand	1352-1355	4	30
α-helix	1365-1366	2
β-strand	1367	1	29
α-helix	1368-1371	4
α-helix	1375-1398	24
α-helix	1409-1411	3
α-helix	1419-1422	4
α-helix	1427-1430	4
α-helix	1436-1439	4
α-helix	1468-1478	11
α-helix	1491-1493	3
α-helix	1520-1537	18
α-helix	1539-1542	4
β-strand	1553-1554	2	31
β-strand	1561-1562	2	31
α-helix	1567-1576	10
α-helix	1581-1597	17
β-strand	1606-1611	6	32
α-helix	1616-1620	5
β-strand	1626	1	33
β-strand	1629-1633	5	32
β-strand	1638-1639	2	34
β-strand	1643-1644	2	32
β-strand	1653	1	34
β-strand	1655-1656	2	34
β-strand	1659-1667	9	32
α-helix	1676-1688	13
β-strand	1694	1	33
β-strand	1700-1706	7	32
β-strand	1711-1716	6	32
α-helix	1722-1734	13
α-helix	1738-1750	13
β-strand	1758-1759	2	35
α-helix	1760-1762	3
β-strand	1763	1	36
α-helix	1768-1772	5
β-strand	1777-1781	5	37
β-strand	1787-1792	6	38
β-strand	1798-1803	6	38
β-strand	1805-1810	6	37
β-strand	1816-1822	7	37
α-helix	1824-1826	3
α-helix	1833-1851	19
β-strand	1860-1863	4	37
α-helix	1866-1868	3
α-helix	1869-1875	7
β-strand	1883-1886	4	37
β-strand	1887	1	36
α-helix	1893-1898	6
α-helix	1900-1907	8
β-strand	1914-1918	5	37
α-helix	1923-1926	4
α-helix	1929-1945	17
α-helix	1949-1954	6
α-helix	1975-1994	20
α-helix	1999-2001	3
α-helix	2004-2012	9
α-helix	2014-2016	3
α-helix	2071-2080	10
α-helix	2083-2086	4
β-strand	2090-2092	3	39
β-strand	2103-2107	5	39
α-helix	2108-2117	10
β-strand	2125-2131	7	39
α-helix	2132-2133	2
β-strand	2139-2146	8	39
β-strand	2150	1	40
β-strand	2151-2152	2	41
β-strand	2157-2158	2	41
α-helix	2163-2164	2
α-helix	2167-2169	3
β-strand	2173-2181	9	39
α-helix	2190-2202	13
β-strand	2212-2219	8	39
β-strand	2222-2230	9	39
α-helix	2232-2240	9
α-helix	2253-2255	3
β-strand	2256-2258	3	39
β-strand	2260-2264	5	39
β-strand	2270-2274	5	39
β-strand	2280	1	40
β-strand	2296-2298	3	39
α-helix	2300-2303	4
α-helix	2307-2309	3
α-helix	2311-2314	4
α-helix	2321-2325	5

Chain b: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	16-21	6	17
β-strand	26-34	9	17
β-strand	40-47	8	17
β-strand	68-72	5	17
α-helix	74-76	3
β-strand	77-82	6	17
α-helix	85-86	2

Chain B: 89 helices, 70 β-strands

Element	Residues	Length	Sheet
β-strand	435-438	4	43
β-strand	442-447	6	43
α-helix	452-456	5
β-strand	463	1	44
β-strand	480	1	44
α-helix	483-494	12
β-strand	499-502	4	45
α-helix	510-523	14
β-strand	537-541	5	45
α-helix	545-559	15
β-strand	568	1	45
α-helix	580-582	3
β-strand	586-588	3	45
α-helix	590-599	10
β-strand	609-614	6	45
α-helix	617-621	5
α-helix	625-642	18
β-strand	647-652	6	45
α-helix	658-664	7
α-helix	669-672	4
β-strand	673-675	3	45
α-helix	678-680	3
β-strand	685-692	8	43
α-helix	697-714	18
β-strand	721-724	4	43
α-helix	730-734	5
α-helix	737-744	8
α-helix	761-766	6
α-helix	774-777	4
β-strand	782-784	3	43
α-helix	792-802	11
β-strand	808-811	4	43
α-helix	813-817	5
β-strand	823	1	46
β-strand	825-829	5	43
β-strand	832-835	4	47
β-strand	840-843	4	47
α-helix	844-845	2
α-helix	846-853	8
β-strand	856	1	46
β-strand	865-872	8	43
α-helix	873-875	3
α-helix	876-883	8
α-helix	893-906	14
β-strand	912	1	48
α-helix	913-920	8
α-helix	924-931	8
α-helix	933-936	4
α-helix	940-945	6
α-helix	950-955	6
α-helix	958-966	9
β-strand	970-972	3	49
β-strand	978	1	48
β-strand	979-981	3	49
α-helix	983-991	9
α-helix	995-1004	10
α-helix	1011-1019	9
α-helix	1022-1024	3
α-helix	1031-1033	3
α-helix	1034-1042	9
α-helix	1055-1067	13
α-helix	1075-1102	28
β-strand	1104	1	50
α-helix	1105-1120	16
α-helix	1128-1131	4
α-helix	1137-1145	9
α-helix	1150-1152	3
α-helix	1158-1162	5
α-helix	1168-1170	3
α-helix	1171-1180	10
β-strand	1184-1194	11	51
β-strand	1197-1206	10	51
β-strand	1218-1226	9	52
β-strand	1232	1	50
β-strand	1233-1242	10	52
α-helix	1249	1
β-strand	1250-1257	8	51
β-strand	1265-1272	8	52
β-strand	1279-1285	7	52
α-helix	1291-1302	12
α-helix	1305-1307	3
β-strand	1308	1	53
α-helix	1315-1318	4
β-strand	1327	1	53
α-helix	1330-1339	10
β-strand	1346-1349	4	54
α-helix	1356-1370	15
β-strand	1376-1379	4	54
α-helix	1383-1393	11
α-helix	1394-1399	6
α-helix	1400-1401	2
β-strand	1405-1407	3	54
α-helix	1412-1418	7
β-strand	1419	1	55
β-strand	1422	1	55
β-strand	1424-1427	4	54
α-helix	1431-1437	7
α-helix	1443-1446	4
β-strand	1450-1454	5	54
α-helix	1456-1460	5
α-helix	1465-1480	16
β-strand	1486-1491	6	54
β-strand	1494	1	56
α-helix	1497-1504	8
α-helix	1508-1510	3
β-strand	1511-1513	3	54
β-strand	1523-1530	8	57
α-helix	1535-1541	7
α-helix	1543-1553	11
β-strand	1559-1563	5	57
α-helix	1568-1582	15
β-strand	1590	1	58
α-helix	1594-1601	8
α-helix	1607-1614	8
β-strand	1619-1620	2	57
α-helix	1626-1638	13
β-strand	1641	1	58
β-strand	1643-1647	5	57
α-helix	1648-1651	4
β-strand	1658	1	59
β-strand	1660-1664	5	57
β-strand	1667-1670	4	60
β-strand	1675-1678	4	60
α-helix	1679-1680	2
α-helix	1681-1689	9
β-strand	1691	1	59
β-strand	1700-1707	8	57
α-helix	1711-1717	7
α-helix	1721	1
β-strand	1722	1	56
α-helix	1723	1
α-helix	1733-1741	9
β-strand	1747	1	61
α-helix	1748-1755	8
α-helix	1759-1764	6
α-helix	1768-1770	3
α-helix	1778-1798	21
β-strand	1802-1805	4	61
β-strand	1809-1812	4	61
α-helix	1814-1822	9
α-helix	1826-1835	10
α-helix	1842-1850	9
α-helix	1853-1855	3
α-helix	1867-1873	7
α-helix	1887-1899	13
α-helix	1906-1933	28
β-strand	1935	1	62
α-helix	1936-1951	16
α-helix	1960-1962	3
α-helix	1968-1977	10
α-helix	2001-2013	13
β-strand	2017-2023	7	63
β-strand	2034-2043	10	63
β-strand	2064-2070	7	62
α-helix	2071-2073	3
β-strand	2075-2082	8	62
β-strand	2087-2095	9	63
β-strand	2102-2110	9	62
β-strand	2118-2124	7	62

Chain c: 13 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	32-44	13
α-helix	49-50	2
β-strand	51-52	2	18
α-helix	57	1
β-strand	58-59	2	18
α-helix	60	1
α-helix	87-88	2
α-helix	96-97	2
α-helix	153-157	5
α-helix	163-166	4
α-helix	175-178	4
α-helix	179-199	21
α-helix	273-275	3
β-strand	285-286	2	19
β-strand	291-292	2	19
α-helix	320-337	18
α-helix	347-361	15

Chain C: 34 helices, 43 β-strands

Element	Residues	Length	Sheet
α-helix	69-72	4
β-strand	78-81	4	64
β-strand	103	1	65
α-helix	116-123	8
β-strand	129-136	8	66
α-helix	142-148	7
β-strand	171	1	65
α-helix	172-176	5
β-strand	184-191	8	66
β-strand	197-204	8	66
α-helix	209-211	3
α-helix	213-220	8
β-strand	224-230	7	66
β-strand	234	1	66
α-helix	237-247	11
β-strand	252-258	7	66
α-helix	260-262	3
α-helix	271-292	22
β-strand	307-311	5	66
β-strand	316-318	3	66
α-helix	320-330	11
α-helix	336-340	5
β-strand	348-350	3	23
β-strand	355-357	3	23
β-strand	367	1	23
α-helix	368-369	2
α-helix	370-374	5
α-helix	375-385	11
α-helix	393-399	7
α-helix	412-423	12
α-helix	429-436	8
α-helix	439-440	2
α-helix	441-444	4
α-helix	446-452	7
β-strand	453	1	67
β-strand	476-482	7	68
β-strand	491-496	6	68
β-strand	501-502	2	69
β-strand	506-510	5	68
β-strand	515	1	70
β-strand	518	1	70
β-strand	522-526	5	68
β-strand	530-533	4	68
β-strand	538-541	4	68
β-strand	543-544	2	69
β-strand	549-552	4	68
β-strand	563-566	4	68
β-strand	577	1	67
α-helix	578-580	3
β-strand	588-594	7	71
α-helix	597-599	3
α-helix	600-613	14
β-strand	618-621	4	71
β-strand	627-631	5	71
α-helix	634-642	9
α-helix	643-647	5
β-strand	653-655	3	71
α-helix	656-658	3
β-strand	659-660	2	71
β-strand	663-666	4	72
β-strand	674-675	2	73
β-strand	684-691	8	73
α-helix	697-701	5
α-helix	713-722	10
β-strand	744-747	4	73
α-helix	763-776	14
β-strand	784	1	72
β-strand	787-795	9	73
α-helix	809-820	12
β-strand	825-839	15	72
α-helix	843-852	10
β-strand	859-862	4	72
β-strand	868-876	9	72
α-helix	883-889	7
β-strand	896-906	11	72
α-helix	926-928	3
α-helix	929-941	13

41 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
U2 snRNA	2	RNA	189	Homo sapiens
U5 snRNA	5	RNA	116	Homo sapiens
U6 snRNA	6	RNA	106	Homo sapiens
Eukaryotic initiation factor 4A-III, N-terminally processed	7	protein	390	Homo sapiens	P38919 (AlphaFold model)
RNA-binding protein 8A	8	protein	91	Homo sapiens	Q9Y5S9 (AlphaFold model)
Protein mago nashi homolog 2	9	protein	144	Homo sapiens	Q96A72 (AlphaFold model)
Pre-mRNA-processing-splicing factor 8	A	protein	2335	Homo sapiens	Q6P2Q9 (AlphaFold model)
U5 small nuclear ribonucleoprotein 200 kDa helicase	B	protein	1722	Homo sapiens	O75643
116 kDa U5 small nuclear ribonucleoprotein component	C	protein	899	Homo sapiens	Q15029
PRKR-interacting protein 1	D	protein	123	Homo sapiens	Q9H875
Ligated exons: MINX mRNA	E	RNA	14	Human adenovirus 2
Cactin	F	protein	122	Homo sapiens	Q8WUQ7

32 more molecules are not listed.

Sequence of entity 1 (2), FASTA

>6QDV_1 U2 snRNA (chains 2)
AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAU
CUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAAGAUUUUUGGAACUCAGGGAGAUGG
AAUAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAAC
GGUGCACCC

Sequence of entity 2 (5), FASTA

>6QDV_2 U5 snRNA (chains 5)
AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGG
AGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGCCUUGCCUUGGCAAGGCUA

Sequence of entity 3 (6), FASTA

>6QDV_3 U6 snRNA (chains 6)
GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGC
CCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUU

Sequence of entity 4 (7), FASTA

>6QDV_4 Eukaryotic initiation factor 4A-III, N-terminally processed (chains 7)
DMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQ
SQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACI
GGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQI
YDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVERE
EWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMK
EFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFV
KNDDIRILRDIEQYYSTQIDEMPMNVADLI

Sequence of entity 5 (8), FASTA

>6QDV_5 RNA-binding protein 8A (chains 8)
PGPQRSVEGWILFVTGVHEEATEEDIHDKFAEYGEIKNIHLNLDRRTGYLKGYTLVEYET
YKEAQAAMEGLNGQDLMGQPISVDWCFVRGP

Sequence of entity 6 (9), FASTA

>6QDV_6 Protein mago nashi homolog 2 (chains 9)
SDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEELKR
IIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEGLRVF
YYLVQDLKCLVFSLIGLHFKIKPI

Sequence of entity 7 (A), FASTA

>6QDV_7 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Sequence of entity 8 (B), FASTA

>6QDV_8 U5 small nuclear ribonucleoprotein 200 kDa helicase (chains B)
APRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLP
VEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGK
HINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISAT
QIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQ
EDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQI
MNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLR
TEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPA
HTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLL
NQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDL
KGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQ
LLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLL
QAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMW
QSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPK
LELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKA
KYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLD
LQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFA
ILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGN
IIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIER
PIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMA
KPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEK
LSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVII
MDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLP
VESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSD
HLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVR
GLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQ
LSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQL
PHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEV
VDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLT
LQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVD

Sequence of entity 9 (C), FASTA

>6QDV_9 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
EVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYE
MDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQ
ERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGV
MLNTERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTD
ENLILSPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRK
FTKKAPTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPL
LRLVCKKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLM
CHTTKMYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVA
RYHIEVNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPV
NPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKV
ADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLG
EFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTR
EGPLCDELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVE
VQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQA
FSLSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSIKFFDD

Sequence of entity 10 (D), FASTA

>6QDV_10 PRKR-interacting protein 1 (chains D)
TLVIPKNAAEEQKLKLERLMKNPDKAVPIPEKMSEWAPRPPPEFVRDVMGSSAGAGSGEF
HVYRHLRRREYQRQDYMDAMAEKQKLDAEFQKRLEKNKIAAEEQTAKRRKKRQKLKEKKL
LAK

Sequence of entity 11 (E), FASTA

>6QDV_11 Ligated exons: MINX mRNA (chains E)
GGCCUCCGAACGCU

Sequence of entity 12 (F), FASTA

>6QDV_12 Cactin (chains F)
KYRPRKPRFFNRVHTGFAWNKYNQTHYDFDNPPPKIVQGYKFNIFYPDLIDKRSTPEYFL
EACADNKDFAILRFHAGPPYEDIAFKIVNREWEYSHRHGFRCQFANGIFQLWFHFKRYRY
RR

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	7
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	1
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
ZN	Zinc ion	Zn	7
IHP	Inositol hexakisphosphate	C6 H18 O24 P6	1

Water and common crystallization additives (K) are not listed.

Primary citation

A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation. Fica, S.M., Oubridge, C., Wilkinson, M.E. et al. Science (2019) 363:710-714. DOI 10.1126/science.aaw5569 · PubMed

Other PDB entries of the same protein (UniProt P38919 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4C9B 2.0 Å, Crystal structure of eIF4AIII-CWC22 complex
2J0S 2.21 Å, The crystal structure of the Exon Junction Complex at 2.2 A resolution
2HYI 2.3 Å, Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA
3EX7 2.3 Å, The crystal structure of EJC in its transition state
7ZNJ 2.4 Å, Structure of an ALYREF-exon junction complex hexamer
8C6J 2.8 Å, Human spliceosomal PM5 C* complex
9XTT 2.92 Å, Human minor spliceosome branching-completed C complex (after step-I)
2J0U 3.0 Å, The crystal structure of eIF4AIII-Barentsz complex at 3.0 A resolution
6ICZ 3.0 Å, Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom
9XU3 3.0 Å, Human minor spliceosome exon-ligation-ready C* complex (prior to step-II)
6YVH 3.19 Å, CWC22-CWC27-EIF4A3 Complex
2J0Q 3.2 Å, The crystal structure of the Exon Junction Complex at 3.2 A resolution

Browse structure collections

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