CryoEM structure of a beta3K279T GABA(A)R homomer in complex with histamine and megabody Mb25. Determined by electron microscopy at 2.49 Å resolution. Released 4 Aug 2021.
Explore 6QFA in 3D Show helices and sheets RCSB PDB PDBe
6QFA contains 75 α-helices and 115 β-strands across 10 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 10-20 | 11 | |
| α-helix | 35 | 1 | |
| β-strand | 36-51 | 16 | 20 |
| β-strand | 56-68 | 13 | 20 |
| α-helix | 70-72 | 3 | |
| β-strand | 82-83 | 2 | 20 |
| α-helix | 85-90 | 6 | |
| β-strand | 96-98 | 3 | 19 |
| β-strand | 101-106 | 6 | 20 |
| β-strand | 110 | 1 | 2 |
| β-strand | 114-118 | 5 | 20 |
| β-strand | 123-135 | 13 | 20 |
| β-strand | 147-156 | 10 | 19 |
| β-strand | 164-168 | 5 | 20 |
| α-helix | 171-173 | 3 | |
| β-strand | 175-176 | 2 | 20 |
| β-strand | 186-199 | 14 | 19 |
| β-strand | 204-216 | 13 | 19 |
| α-helix | 217 | 1 | |
| α-helix | 219-221 | 3 | |
| α-helix | 222-226 | 5 | |
| α-helix | 227-235 | 9 | |
| α-helix | 238-241 | 4 | |
| α-helix | 247-270 | 24 | |
| α-helix | 280-305 | 26 | |
| α-helix | 310-446 | 30 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2-7 | 6 | 4 |
| β-strand | 405-413 | 9 | 4 |
| β-strand | 420-426 | 7 | 5 |
| α-helix | 431 | 1 | |
| β-strand | 432-439 | 8 | 5 |
| β-strand | 445-447 | 3 | 5 |
| β-strand | 455-460 | 6 | 4 |
| β-strand | 465-470 | 6 | 4 |
| β-strand | 479-486 | 8 | 5 |
| α-helix | 496-498 | 3 | |
| β-strand | 499-502 | 4 | 5 |
| β-strand | 506-508 | 3 | 5 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3 | A, B, C, D, E | protein | 341 | Homo sapiens | P28472 (AlphaFold model) |
| Outer membrane protein,Outer membrane protein,Outer membrane protein,Outer membrane… | K, L, M, N, O | protein | 522 | Helicobacter pylori (strain G27), Lama glama | B5Z8H1 (AlphaFold model) |
>6QFA_1 Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3 (chains A, B, C, D, E) QSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLT MYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHP DGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVER IELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSF WINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVTAIDMYLMGCFVFVFLALLEYA FVNYIFFSQPARAAAIDRWSRIVFPFTFSLFNLVYWLYYVN
>6QFA_2 Outer membrane protein,Outer membrane protein,Outer membrane protein,Outer membrane protein,Uncharacterized protein,Uncharacterized protein,Mb-c7HopQ-Nb25 (chains K, L, M, N, O) QVQLVESGGGLVQTKTTTSVIDTTNDAQNLLTQAQTIVNTLKDYCPILIAKSSSSNGGTN NANTPSWQTAGGGKNSCATFGAEFSAASDMINNAQKIVQETQQLSANQPKNITQPHNLNL NSPSSLTALAQKMLKNAQSQAEILKLANQVESDFNKLSSGHLKDYIGKCDASAISSANMT MQNQKNNWGNGCAGVEETQSLLKTSAADFNNQTPQINQAQNLANTLIQELGNNTYEQLSR LLTNDNGTNSKTSAQAINQAVNNLNERAKTLAGGTTNSPAYQATLLALRSVLGLWNSMGY AVICGGYTKSPGENNQKDFHYTDENGNGTTINCGGSTNSNGTHSYNGTNTLKADKNVSLS IEQYEKIHEAYQILSKALKQAGLAPLNSKGEKLEAHVTTSKYGSLRLSCAASGHTFNYPI MGWFRQAPGKEREFVGAISWSGGSTSYADSVKDRFTISRDNAKNTVYLEMNNLKPEDTAV YYCAAKGRYSGGLYYPTNYDYWGQGTQVTVSSHHHHHHEPEA
Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM. Uchanski, T., Masiulis, S., Fischer, B. et al. Nat Methods (2021) 18:60-68. DOI 10.1038/s41592-020-01001-6 · PubMed
Other PDB entries of the same protein (UniProt P28472 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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