Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the long-lived symmetric desensitised state. Determined by electron microscopy at 2.5 Å resolution. Released 4 Jun 2025.
Explore 9FEU in 3D Show helices and sheets RCSB PDB PDBe
9FEU contains 60 α-helices and 65 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 9-20 | 12 | |
| α-helix | 35 | 1 | |
| β-strand | 36-51 | 16 | 1 |
| β-strand | 56-68 | 13 | 1 |
| α-helix | 70-72 | 3 | |
| β-strand | 81-83 | 3 | 1 |
| α-helix | 85-90 | 6 | |
| β-strand | 96-98 | 3 | 2 |
| β-strand | 101-106 | 6 | 1 |
| β-strand | 110 | 1 | 3 |
| β-strand | 114-118 | 5 | 1 |
| β-strand | 123-135 | 13 | 1 |
| β-strand | 147-156 | 10 | 2 |
| β-strand | 164-168 | 5 | 1 |
| α-helix | 171-173 | 3 | |
| β-strand | 175-176 | 2 | 1 |
| β-strand | 186-200 | 15 | 2 |
| β-strand | 203-216 | 14 | 2 |
| α-helix | 219-221 | 3 | |
| α-helix | 222-226 | 5 | |
| α-helix | 227-236 | 10 | |
| α-helix | 237-241 | 5 | |
| α-helix | 247-271 | 25 | |
| α-helix | 280-305 | 26 | |
| α-helix | 310-445 | 29 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Gamma-aminobutyric acid receptor subunit beta-3 | A, B, C, D, E | protein | 395 | Homo sapiens | P28472 (AlphaFold model) |
>9FEU_1 Gamma-aminobutyric acid receptor subunit beta-3 (chains A, B, C, D, E) MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPDYDIPTTENLYFQGTGQSVNDP GNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQY WRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLY GLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQF SIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYDA SAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIF FSQPARAAAIDRWSRIVFPFTFSLFNLVYWLYYVN
| ID | Name | Formula | Copies |
|---|---|---|---|
| D10 | Decane | C10 H22 | 15 |
| HSM | Histamine | C5 H9 N3 | 5 |
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 5 |
Water and common crystallization additives (CL) are not listed.
Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the long-lived symmetric desensitised state. Mihaylov, D.B., Malinauskas, T., Aricescu, A.R. To be published.
Other PDB entries of the same protein (UniProt P28472 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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