6RLP: Cryo-EM reconstruction of TMV coat protein

Cryo-EM reconstruction of TMV coat protein. Determined by electron microscopy at 2.3 Å resolution. Released 23 Nov 2022.

Method: Electron microscopy
Resolution: 2.3 Å
Organism: Tobacco mosaic virus
Chains: 48
Atoms: 30,552
Mol. weight: 433.22 kDa
Released: 23 Nov 2022

Explore 6RLP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6RLP contains 216 α-helices and 144 β-strands across 24 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, S, T, U, V, W, X and Y: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	1
α-helix	7-13	7
β-strand	17-18	2	2
α-helix	20-30	11
α-helix	38-49	12
β-strand	53	1	2
α-helix	61-63	3
β-strand	68-70	3	2
α-helix	76-86	11
α-helix	93-96	4
α-helix	101-103	3
α-helix	104-132	29
β-strand	138-139	2	2
α-helix	143-148	6
β-strand	152	1	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Capsid protein	A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, S, T, U, V, W, X, Y	protein	153	Tobacco mosaic virus	P69687
RNA (5'-r(pgpap*a)-3')	R, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v	RNA	3	Tobacco mosaic virus

Sequence of entity 1 (A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, S, T, U, V, W, X, Y), FASTA

>6RLP_1 Capsid protein (chains A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, S, T, U, V, W, X, Y)
SYSITTPSQFVFLSSAWADPIELINLCTNALGNQFQTQQARTVVQRQFSEVWKPSPQVTV
RFPDSDFKVYRYNAVLDPLVTALLGAFDTRNRIIEVENQANPTTAETLDATRRVDDATVA
IRSAINNLIVELIRGTGSYNRSSFESSSGLVWT

Sequence of entity 2 (R, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v), FASTA

>6RLP_2 RNA (5'-R(P*GP*AP*A)-3') (chains R, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v)
GAA

Primary citation

CM01: a facility for cryo-electron microscopy at the European Synchrotron. Kandiah, E., Giraud, T., de Maria Antolinos, A. et al. Acta Crystallogr D Struct Biol (2019) 75:528-535. DOI 10.1107/S2059798319006880 · PubMed

Other PDB entries of the same protein (UniProt P69687), best resolution first:

6SAE 1.9 Å, Cryo-EM structure of TMV in water
6R7M 1.92 Å, Tobacco Mosaic Virus (TMV)
6SAG 2.0 Å, Cryo-EM structure of TMV with Ca2+ at low pH
6I5A 2.3 Å, Tobacco Mosaic Virus
1EI7 2.45 Å, Tmv coat protein refined from the 4-layer aggregate
8EAW 2.8 Å, An asymmetric disk assembly formed by tandem dimers of the tobacco mosaic viral capsid…
2TMV 2.9 Å, Visualization of protein-nucleic acid interactions in a virus. Refined structure of…
6X0Q 3.0 Å, A Circular Permutant of the Tobacco Mosaic Virus (TMV) mutant Q101H coordinated with heme
6X0R 3.0 Å, A Circular Permutant of the Tobacco Mosaic Virus (TMV) mutant Q101H
3KML 3.01 Å, Circular Permutant of the Tobacco Mosaic Virus
4GQH 3.06 Å, The Conformations and Interactions of the Four-Layer Aggregate Revealed by X-ray…
3J06 3.3 Å, CryoEM Helical Reconstruction of TMV

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