6RMV: TRP channel peptide

The crystal structure of a TRP channel peptide bound to a G protein beta gamma heterodimer. Determined by X-ray diffraction at 1.94 Å resolution. Released 14 Oct 2020.

Method: X-ray diffraction
Resolution: 1.94 Å
Organism: Mus musculus
Chains: 3
Atoms: 3,313
Mol. weight: 47.29 kDa
Released: 14 Oct 2020

Explore 6RMV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6RMV contains 10 α-helices and 28 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	6-25	20
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	1
β-strand	58-63	6	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	89-94	6	2
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	121-125	5	3
β-strand	134-139	6	3
β-strand	146-153	8	4
β-strand	156-161	6	4
β-strand	166-170	5	4
β-strand	175-180	6	4
β-strand	187-192	6	5
β-strand	198-203	6	5
β-strand	208-212	5	5
β-strand	218-222	5	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	250-254	5	6
β-strand	259-264	6	6
α-helix	272	1
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	304-308	5	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-339	4	1

Chain B: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	10-23	14
α-helix	30-44	15
α-helix	45-47	3
α-helix	53-55	3
α-helix	62-64	3

Chain C: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	593-600	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	A	protein	340	Mus musculus	P62874 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	B	protein	74	Mus musculus	P63213 (AlphaFold model)
Transient receptor potential cation channel, subfamily M, member 3	C	protein	15	Mus musculus	Q2TV84 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6RMV_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains A)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (B), FASTA

>6RMV_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains B)
GSGMASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPAS
ENPFREKKFFSAIL

Sequence of entity 3 (C), FASTA

>6RMV_3 Transient receptor potential cation channel, subfamily M, member 3 (chains C)
KRPKALKLLGMEDDI

Primary citation

The structural basis for an on-off switch controlling G beta gamma-mediated inhibition of TRPM3 channels. Behrendt, M., Gruss, F., Enzeroth, R. et al. Proc Natl Acad Sci U S A (2020) 117:29090-29100. DOI 10.1073/pnas.2001177117 · PubMed

Other PDB entries of the same protein (UniProt P62874 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8IHB 2.85 Å, Cryo-EM structure of HCA2-Gi complex with GSK256073
22EM 2.86 Å, Gi bound kappa-opioid receptor in complex with beta01
6K41 2.9 Å, cryo-EM structure of alpha2BAR-GoA complex
7Y15 2.9 Å, Cryo-EM structure of apo-state MrgD-Gi complex
8IHF 2.97 Å, Cryo-EM structure of HCA2-Gi complex with MK6892
8IHH 3.06 Å, Cryo-EM structure of HCA2-Gi complex with LUF6283
8IHJ 3.07 Å, Cryo-EM structure of HCA3-Gi complex with acifran
7Y12 3.1 Å, Cryo-EM structure of MrgD-Gi complex with beta-alanine
8IHI 3.11 Å, Cryo-EM structure of HCA2-Gi complex with acifran
6K42 4.1 Å, cryo-EM structure of alpha2BAR-Gi1 complex

Browse structure collections

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