6SQC: PDB entry 6SQC

Crystal structure of complex between nuclear coactivator binding domain of CBP and [1040-1086]ACTR containing alpha-methylated Leu1055 and Leu1076. Determined by X-ray diffraction at 2.28 Å resolution. Released 30 Sept 2020.

Method: X-ray diffraction
Resolution: 2.28 Å
Organisms: Escherichia coli (strain K12), Homo sapiens
Chains: 2
Atoms: 3,759
Mol. weight: 51.96 kDa
Ligands: ZN
Released: 30 Sept 2020

Explore 6SQC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6SQC contains 32 α-helices and 24 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 29 helices, 24 β-strands

Element	Residues	Length	Sheet
α-helix	1690-1692	3
β-strand	1696-1699	4	1
α-helix	1706-1720	15
β-strand	1724-1727	4	1
α-helix	1732-1739	8
α-helix	1740-1742	3
β-strand	1748-1752	5	1
α-helix	1753-1755	3
α-helix	1756-1761	6
β-strand	1765	1	2
α-helix	1766-1767	2
β-strand	1768	1	3
α-helix	1772-1775	4
β-strand	1778	1	4
α-helix	1780-1785	6
β-strand	1787-1788	2	3
β-strand	1791-1792	2	3
β-strand	1795-1800	6	1
β-strand	1803-1807	5	5
β-strand	1817	1	6
α-helix	1821-1829	9
β-strand	1834-1836	3	5
α-helix	1843-1851	9
β-strand	1856-1861	6	7
β-strand	1864-1871	8	7
α-helix	1875-1889	15
α-helix	1899-1907	9
β-strand	1911-1916	6	5
α-helix	1918-1920	3
α-helix	1921-1926	6
β-strand	1931-1934	4	5
α-helix	1935-1937	3
β-strand	1938-1939	2	6
β-strand	1942-1943	2	6
α-helix	1944	1
β-strand	1947-1948	2	8
β-strand	1949-1955	7	1
β-strand	1956	1	2
α-helix	1962-1968	7
α-helix	1969-1973	5
α-helix	1976-1985	10
β-strand	1990-1991	2	1
β-strand	1993	1	4
α-helix	1994-1999	6
α-helix	2004-2015	12
β-strand	2017-2018	2	8
α-helix	2019-2020	2
α-helix	2025-2040	16
α-helix	2046-2055	10
α-helix	2064-2065	2
α-helix	2066-2074	9
α-helix	2080-2092	13
α-helix	2094-2110	17

Chain B: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	1044-1058	15
α-helix	1062-1070	9
α-helix	1073-1080	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Maltose/maltodextrin-binding periplasmic protein,CREB-binding protein	A	protein	424	Escherichia coli (strain K12), Homo sapiens	P0AEX9 (AlphaFold model), Q92793 (AlphaFold model)
Nuclear receptor coactivator 3	B	protein	47	Homo sapiens	Q9Y6Q9 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6SQC_1 Maltose/maltodextrin-binding periplasmic protein,CREB-binding protein (chains A)
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDI
IFWAHDRFGGYAQSGLLAEITPAAAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNK
DLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIK
DVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSA
VNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDA
ALAAAQTNAAAMSISPSALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAAFIKQRTAKY
VANQ

Sequence of entity 2 (B), FASTA

>6SQC_2 Nuclear receptor coactivator 3 (chains B)
EGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPK

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	2

Water and common crystallization additives (EDO) are not listed.

Primary citation

Conformational editing of intrinsically disordered protein by alpha-methylation. Bauer, V., Schmidtgall, B., Gogl, G. et al. Chem Sci (2020) 12:1080-1089. DOI 10.1039/d0sc04482b · PubMed

Other PDB entries of the same protein (UniProt P0AEX9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8C8F 1.15 Å, Crystal structure of the E. coli maltodextrin-binding protein
4EXK 1.28 Å, A chimera protein containing MBP fused to the C-terminal domain of the uncharacterized…
3Q27 1.3 Å, Cyrstal structure of human alpha-synuclein (32-57) fused to maltose binding protein (MBP)
7KD4 1.31 Å, Structure of the C-terminal domain of the Menangle virus phosphoprotein (residues 329…
5M13 1.37 Å, Synthetic nanobody in complex with MBP
5HZ7 1.43 Å, High-resolution crystal structure of the minor DNA-binding pilin ComP from Neisseria…
5H7Q 1.45 Å, Crystal structure of human MNDA PYD domain with MBP tag
6XDS 1.47 Å, Crystal structure of MBP-TREM2 Ig domain fusion with fragment,…
4IRL 1.47 Å, X-ray structure of the CARD domain of zebrafish GBP-NLRP1 like protein
8SVY 1.47 Å, MBP-Mcl1 in complex with ligand 10
3MP6 1.48 Å, Complex Structure of Sgf29 and dimethylated H3K4
9CLC 1.48 Å, Crystal structure of maltose binding protein (Apo), mutant Trp10 to 4-Cyanotryptophan

Browse structure collections

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