6ULG: FLCN-FNIP2-Rag-Ragulator complex

Cryo-EM structure of the FLCN-FNIP2-Rag-Ragulator complex. Determined by electron microscopy at 3.31 Å resolution. Released 20 Nov 2019.

Method: Electron microscopy
Resolution: 3.31 Å
Organism: Homo sapiens
Chains: 9
Atoms: 16,351
Mol. weight: 334 kDa
Ligands: MG, GNP, GDP
Released: 20 Nov 2019

Explore 6ULG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6ULG contains 76 α-helices and 78 β-strands across 9 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	4-12	9
α-helix	13-15	3
β-strand	19-25	7	5
β-strand	31-36	6	5
α-helix	47-50	4
α-helix	52-60	9
β-strand	68-75	8	5
β-strand	78-85	8	5
β-strand	88-95	8	5
α-helix	100-109	10
α-helix	111-117	7
α-helix	118-120	3

Chain B: 3 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	6
α-helix	4-12	9
β-strand	21-25	5	5
β-strand	31-36	6	5
α-helix	42-63	22
β-strand	69-76	8	5
β-strand	79-84	6	5
β-strand	90-94	5	5
α-helix	101-122	22

Chain C: 3 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	3-13	11
β-strand	20-24	5	7
β-strand	30-34	5	7
α-helix	39-41	3
α-helix	43-53	11
β-strand	64	1	8
β-strand	65-69	5	7
β-strand	72-77	6	7
β-strand	83-88	6	7

Chain D: 2 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-9	3
β-strand	15-22	8	7
β-strand	25-31	7	7
α-helix	37-51	15
β-strand	65-68	4	7
β-strand	69	1	8
β-strand	73-80	8	7
β-strand	83-90	8	7

Chain E: 7 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	50-64	15
α-helix	65-67	3
α-helix	79-95	17
α-helix	96-98	3
α-helix	108-110	3
α-helix	115-119	5
α-helix	126-143	18
β-strand	155	1	6

Chain F: 9 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	9-13	5	9
α-helix	22-27	6
α-helix	71-76	6
α-helix	77-81	5
β-strand	85-93	9	9
α-helix	99-116	18
β-strand	121-127	7	9
α-helix	137-151	15
β-strand	158-161	4	9
α-helix	168-179	12
α-helix	186-197	12
β-strand	202-207	6	10
β-strand	213-218	6	10
α-helix	227-243	17
β-strand	249-255	7	10
β-strand	260-265	6	10
β-strand	270-276	7	10
α-helix	283-297	15

Chain G: 11 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	63-66	4	11
β-strand	68	1	11
α-helix	75-82	8
α-helix	89-91	3
α-helix	94-96	3
β-strand	100-101	2	11
β-strand	113-116	4	11
α-helix	132-135	4
β-strand	139-145	7	11
α-helix	152-167	16
β-strand	172-178	7	11
α-helix	185-204	20
β-strand	213	1	11
β-strand	216-218	3	11
α-helix	224-234	11
α-helix	240-253	14
β-strand	258-263	6	10
β-strand	269-272	4	10
α-helix	276-277	2
α-helix	279-299	21
β-strand	315-320	6	10
β-strand	324-330	7	10
β-strand	335-342	8	10
α-helix	350-366	17

Chain L: 18 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	4-10	7	1
β-strand	16-22	7	1
α-helix	92-94	3
β-strand	95-99	5	2
β-strand	104-108	5	2
α-helix	115-129	15
β-strand	140-144	5	1
β-strand	150-159	10	1
β-strand	167-176	10	1
α-helix	179-183	5
α-helix	186-214	29
α-helix	224-226	3
α-helix	250-266	17
β-strand	271-272	2	3
α-helix	283-290	8
α-helix	349-356	8
α-helix	358-370	13
α-helix	372	1
β-strand	373-377	5	4
α-helix	381-391	11
α-helix	392-394	3
β-strand	402-403	2	4
β-strand	417-420	4	4
α-helix	428-431	4
β-strand	436-441	6	4
β-strand	465-468	4	4
α-helix	481-491	11
α-helix	497-523	27
α-helix	531-538	8
α-helix	544-553	10
α-helix	559-567	9

1 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Folliculin	L	protein	579	Homo sapiens	Q8NFG4 (AlphaFold model)
Ragulator complex protein LAMTOR3	A	protein	124	Homo sapiens	Q9UHA4 (AlphaFold model)
Ragulator complex protein LAMTOR2	B	protein	125	Homo sapiens	Q9Y2Q5 (AlphaFold model)
Ragulator complex protein LAMTOR5	C	protein	91	Homo sapiens	O43504 (AlphaFold model)
Ragulator complex protein LAMTOR4	D	protein	99	Homo sapiens	Q0VGL1
Ragulator complex protein LAMTOR1	E	protein	161	Homo sapiens	Q6IAA8
Ras-related GTP-binding protein A	F	protein	313	Homo sapiens	Q7L523
Ras-related GTP-binding protein C	G	protein	399	Homo sapiens	Q9HB90
Folliculin-interacting protein 2	N	protein	1114	Homo sapiens	Q9P278

Sequence of entity 1 (L), FASTA

>6ULG_1 Folliculin (chains L)
MNAIVALCHFCELHGPRTLFCTEVLHAPLPQGDGNEDSPGQGEQAEEEEGGIQMNSRMRA
HSPAEGASVESSSPGPKKSDMCEGCRSLAAGHPGYISHDKETSIKYVSHQHPSHPQLFSI
VRQACVRSLSCEVCPGREGPIFFGDEQHGFVFSHTFFIKDSLARGFQRWYSIITIMMDRI
YLINSWPFLLGKVRGIIDELQGKALKVFEAEQFGCPQRAQRMNTAFTPFLHQRNGNAARS
LTSLTSDDNLWACLHTSFAWLLKACGSRLTEKLLEGAPTEDTLVQMEKLADLEEESESWD
NSEAEEEEKAPVLPESTEGRELTQGPAESSSLSGCGSWQPRKLPVFKSLRHMRQVLGAPS
FRMLAWHVLMGNQVIWKSRDVDLVQSAFEVLRTMLPVGCVRIIPYSSQYEEAYRCNFLGL
SPHVQIPPHVLSSEFAVIVEVHAAARSTLHPVGCEDDQSLSKYEFVVTSGSPVAADRVGP
TILNKIEAALTNQNLSVDVVDQCLVCLKEEWMNKVKVLFKFTKVDSRPKEDTQKLLSILG
ASEEDNVKLLKFWMTGLSKTYKSHLMSTVRSPTASESRN

Sequence of entity 2 (A), FASTA

>6ULG_2 Ragulator complex protein LAMTOR3 (chains A)
MADDLKRFLYKKLPSVEGLHAIVVSDRDGVPVIKVANDNAPEHALRPGFLSTFALATDQG
SKLGLSKNKSIICYYNTYQVVQFNRLPLVVSFIASSSANTGLIVSLEKELAPLFEELRQV
VEVS

Sequence of entity 3 (B), FASTA

>6ULG_3 Ragulator complex protein LAMTOR2 (chains B)
MLRPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNG
NQAFNEDNLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLT
QVAAS

Sequence of entity 4 (C), FASTA

>6ULG_4 Ragulator complex protein LAMTOR5 (chains C)
MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAAKLTSDPT
DIPVVCLESDNGNIMIQKHDGITVAVHKMAS

Sequence of entity 5 (D), FASTA

>6ULG_5 Ragulator complex protein LAMTOR4 (chains D)
MTSALTQGLERIPDQLGYLVLSEGAVLASSGDLENDEQAASAISELVSTACGFRLHRGMN
VPFKRLSVVFGEHTLLVTVSGQRVFVVKRQNRGREPIDV

Sequence of entity 6 (E), FASTA

>6ULG_6 Ragulator complex protein LAMTOR1 (chains E)
MGCCYSSENEDSDQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAK
TASNIIDVSAADSQGMEQHEYMDRARQYSTRLAVLSSSLTHWKKLPPLPSLTSQPHQVLA
SEPIPFSDLQQVSRIAAYAYSALSQIRVDAKEELVVQFGIP

Sequence of entity 7 (F), FASTA

>6ULG_7 Ras-related GTP-binding protein A (chains F)
MPNTAMKKKVLLMGKSGSGKNSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNL
WDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDA
KIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECACFRTSIWDETLYKAWSSIVYQL
IPNVQQLEMNLRNFAQIIEADEVLLFERATFLVISHYQCKEQRDVHRFEKISNIIKQFKL
SCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKL
ERVDGPKHSLLMR

Sequence of entity 8 (G), FASTA

>6ULG_8 Ras-related GTP-binding protein C (chains G)
MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGVGAGAGGGCGPGGADSS
KPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKIYKDDISNSSFVNFQIWDFPGQ
MDFFDPTFDYEMIFRGTGALIYVIDAQDDYMEALTRLHITVSKAYKVNPDMNFEVFIHKV
DGLSDDHKIETQRDIHQRANDDLADAGLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQL
PTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSSPVDMQSYELCCDMIDVVIDVSCIYG
LKEDGSGSAYDKESMAIIKLNNTTVLYLKEVTKFLALVCILREESFERKGLIDYNFHCFR
KAIHEVFEVGVTSHRSCGHQTSASSLKALTHNGTPRNAI

Sequence of entity 9 (N), FASTA

>6ULG_9 Folliculin-interacting protein 2 (chains N)
MAPTLLQKLFNKRGSSGSSAAASAQGRAPKEGPAFSWSCSEFDLNEIRLIVYQDCDRRGR
QVLFDSKAVQKIEEVTAQKTEDVPIKISAKCCQGSSSVSSSSSSSISSHSSSGGSSHHAK
EQLPKYQYTRPASDVNMLGEMMFGSVAMSYKGSTLKIHYIRSPPQLMISKVFSARMGSFC
GSTNNLQDSFEYINQDPNLGKLNTNQNSLGPCRTGSNLAHSTPVDMPSRGQNEDRDSGIA
RSASLSSLLITPFPSPSSSTSSSSSYQRRWLRSQTTSLENGIIPRRSTDETFSLAEETCS
SNPAMVRRKKIAISIIFSLCEKEEAQRNFQDFFFSHFPLFESHMNRLKSAIEKAMISCRK
IAESSLRVQFYVSRLMEALGEFRGTIWNLYSVPRIAEPVWLTMMSGTLEKNQLCQRFLKE
FTLLIEQINKNQFFAALLTAVLTYHLAWVPTVMPVDHPPIKAFSEKRTSQSVNMLAKTHP
YNPLWAQLGDLYGAIGSPVRLTRTVVVGKQKDLVQRILYVLTYFLRCSELQENQLTWSGN
HGEGDQVLNGSKIITALEKGEVEESEYVVITVRNEPALVPPILPPTAAERHNPWPTGFPE
CPEGTDSRDLGLKPDKEANRRPEQGSEACSAGCLGPASDASWKPQNAFCGDEKNKEAPQD
GSSRLPSCEVLGAGMKMDQQAVCELLKVEMPTRLPDRSVAWPCPDRHLREKPSLEKVTFQ
IGSFASPESDFESRMKKMEERVKACGPSLEASEAADVAQDPQVSRSPFKPGFQENVCCPQ
NRLSEGDEGESDKGFAEDRGSRNDMAADIAGQLSHAADLGTASHGAGGTGGRRLEATRGL
YVKAAEGPVLEPVAPRCVQRGPGLVAGANIPCGDDNKKANFRTEGDIPRNESSDSALGDS
DDEACASAMLDLGHGGDRTGGSLEVELPLPRSQSISTQNVRNFGRSLLAGYCPTYMPDLV
LHGTGSDEKLKQCLVADLVHTVHHPVLDEPIAEAVCIIADTDKWSVQVATSQRKVTDNMK
LGQDVLVSSQVSSLLQSILQLYKLHLPADFCIMHLEDRLQEMYLKSKMLSEYLRGHTRVH
VKELGVVLGIESNDLPLLTAIASTHSPYVAQILL

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	1
GNP	Phosphoaminophosphonic acid-guanylate ester	C10 H17 N6 O13 P3	1
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1

Primary citation

Cryo-EM Structure of the Human FLCN-FNIP2-Rag-Ragulator Complex. Shen, K., Rogala, K.B., Chou, H.T. et al. Cell (2019) 179:1319-1329.e8. DOI 10.1016/j.cell.2019.10.036 · PubMed

Other PDB entries of the same protein (UniProt Q8NFG4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3V42 2.0 Å, Crystal structure of renal tumor suppressor protein, folliculin
8DHB 3.53 Å, Active FLCN GAP complex
6NZD 3.6 Å, Cryo-EM Structure of the Lysosomal Folliculin Complex (FLCN-FNIP2-RagA-RagC-Ragulator)

Browse structure collections

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