6UUS: PDB entry 6UUS

CryoEM Structure of the active Adrenomedullin 2 receptor G protein complex with adrenomedullin peptide. Determined by electron microscopy at 2.4 Å resolution. Released 1 Apr 2020.

Method: Electron microscopy
Resolution: 2.4 Å
Organisms: Homo sapiens, Lama glama
Chains: 7
Atoms: 9,308
Mol. weight: 186.43 kDa
Released: 1 Apr 2020

Explore 6UUS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6UUS contains 39 α-helices and 53 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	17-37	21
β-strand	40-46	7	1
β-strand	207-214	8	1
β-strand	217-224	8	1
α-helix	234-238	5
β-strand	243-248	6	1
α-helix	265-277	13
β-strand	286-291	6	1
α-helix	313-315	3
α-helix	326-327	2
α-helix	332-349	18
β-strand	359-362	4	1
α-helix	372-390	19

Chain B: 1 helix, 28 β-strands

Element	Residues	Length	Sheet
α-helix	8-24	17
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	218-223	6	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	262-264	3	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain E: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	37-47	11
α-helix	59-76	18
α-helix	87-98	12
α-helix	108-110	3
α-helix	120-142	23

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	15-23	9
α-helix	30-43	14
α-helix	56-58	3

Chain N: 1 helix, 13 β-strands

Element	Residues	Length	Sheet
β-strand	5-7	3	9
β-strand	10-11	2	10
β-strand	17-23	7	9
β-strand	34-39	6	10
β-strand	45-51	7	10
β-strand	58-60	3	10
β-strand	65	1	11
β-strand	68	1	11
β-strand	69-73	5	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-98	7	10
β-strand	118	1	10
β-strand	122-125	4	10

Chain P: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	22-32	11
α-helix	42-43	2

Chain R: 20 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	36-51	16
α-helix	65-67	3
β-strand	68-69	2	12
β-strand	74-75	2	12
β-strand	82-83	2	13
β-strand	87	1	14
β-strand	100	1	14
β-strand	104-105	2	13
α-helix	125-128	4
α-helix	134-165	32
α-helix	172-194	23
α-helix	195-199	5
α-helix	202-207	6
α-helix	210-237	28
α-helix	238-242	5
α-helix	245-246	2
α-helix	253-256	4
α-helix	257-261	5
α-helix	264-277	14
α-helix	289-291	3
α-helix	292-319	28
α-helix	330-345	16
α-helix	365-374	10
α-helix	376-381	6
α-helix	382-386	5
α-helix	389-398	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	138	Lama glama
ADM	P	protein	53	Homo sapiens	P35318 (AlphaFold model)
Calcitonin gene-related peptide type 1 receptor	R	protein	490	Homo sapiens	Q16602
Receptor activity-modifying protein 3	E	protein	149	Homo sapiens	O60896

Sequence of entity 1 (A), FASTA

>6UUS_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>6UUS_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>6UUS_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>6UUS_4 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 5 (P), FASTA

>6UUS_5 ADM (chains P)
YRQSMNNFQGLRSFGCRFGTCTVQKLAHQIYQFTDKDKDNVAPRSKISPQGYX

Sequence of entity 6 (R), FASTA

>6UUS_6 Calcitonin gene-related peptide type 1 receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAELEESPEDSIQLGVTRNKIMTAQYECYQ
KIMQDPIQQAEGVYCNRTWDGWLCWNDVAAGTESMQLCPDYFQDFDPSEKVTKICDQDGN
WFRHPASNRTWTNYTQCNVNTHEKVKTALNLFYLTIIGHGLSIASLLISLGIFFYFKSLS
CQRITLHKNLFFSFVCNSVVTIIHLTAVANNQALVATNPVSCKVSQFIHLYLMGCNYFWM
LCEGIYLHTLIVVAVFAEKQHLMWYYFLGWGFPLIPACIHAIARSLYYNDNCWISSDTHL
LYIIHGPICAALLVNLFFLLNIVRVLITKLKVTHQAESNLYMKAVRATLILVPLLGIEFV
LIPWRPEGKIAEEVYDYIMHILMHFQGLLVSTIFCFFNGEVQAILRRNWNQYKIQFGNSF
SNSEALRSASYTVSTISDGPGYSHDCPSEHLNGKSIHDIENVLLKPENLYNPAGLEVLFQ
GPHHHHHHHH

Sequence of entity 7 (E), FASTA

>6UUS_7 Receptor activity-modifying protein 3 (chains E)
MKTIIALSYIFCLVFADYKDDDDKRAGGCNETGMLERLPLCGKAFADMMGKVDVWKWCNL
SEFIVYYESFTNCTEMEANVVGCYWPNPLAQGFITGIHRQFFSNCTVDRVHLEDPPDEVL
IPLIVIPVVLTVAMAGLVVWRSKRTDTLL

Primary citation

Structure and Dynamics of Adrenomedullin Receptors AM1and AM2Reveal Key Mechanisms in the Control of Receptor Phenotype by Receptor Activity-Modifying Proteins. Liang, Y.L., Belousoff, M.J., Fletcher, M.M. et al. Acs Pharmacol Transl Sci (2020) 3:263-284. DOI 10.1021/acsptsci.9b00080 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

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