6VMC: T4H2 T cell receptor

T4H2 T cell receptor bound to HLA-A2 presenting gp100T2L peptide (ILDQVPFSV). Determined by X-ray diffraction at 2.85 Å resolution. Released 20 Jan 2021.

Method: X-ray diffraction
Resolution: 2.85 Å
Organism: Homo sapiens
Chains: 5
Atoms: 6,661
Mol. weight: 95.04 kDa
Released: 20 Jan 2021

Explore 6VMC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6VMC contains 19 α-helices and 76 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	3-12	10	12
α-helix	20	1
β-strand	21-28	8	12
β-strand	31-37	7	12
β-strand	46-47	2	12
α-helix	51-54	4
α-helix	57-84	28
β-strand	94-103	10	12
β-strand	109-118	10	12
β-strand	121-126	6	12
β-strand	133-135	3	12
α-helix	138-148	11
α-helix	152-158	7
α-helix	159-163	5
α-helix	164-174	11
α-helix	176-179	4
β-strand	183	1	13
β-strand	186-193	8	14
β-strand	198-208	11	14
β-strand	209	1	13
β-strand	213-219	7	15
β-strand	222-223	2	15
β-strand	228-230	3	14
α-helix	231-233	3
β-strand	234-235	2	14
β-strand	241-250	10	14
α-helix	254-256	3
β-strand	257-263	7	15
β-strand	270-272	3	15

Chain B: 1 helix, 12 β-strands

Element	Residues	Length	Sheet
β-strand	4	1	16
β-strand	7	1	17
β-strand	10-12	3	17
β-strand	22-31	10	17
β-strand	32	1	16
β-strand	37-42	6	18
β-strand	45-46	2	18
α-helix	47	1
β-strand	51-52	2	17
β-strand	56-57	2	17
β-strand	63-71	9	17
β-strand	79-84	6	18
β-strand	92-95	4	18

Chain C: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	4-8	5

Chain D: 1 helix, 19 β-strands

Element	Residues	Length	Sheet
β-strand	5-6	2	1
β-strand	12-15	4	2
β-strand	20-22	3	1
β-strand	25-26	2	1
β-strand	34-39	6	3
β-strand	46-51	6	3
β-strand	55-59	5	1
β-strand	62-67	6	1
β-strand	72-77	6	1
α-helix	82-84	3
β-strand	87-94	8	3
β-strand	99-102	4	3
β-strand	106-107	2	3
β-strand	108-111	4	2
β-strand	120-125	6	4
β-strand	126	1	5
β-strand	133-140	8	4
β-strand	154-156	3	4
β-strand	160-164	5	4
β-strand	169-178	10	4

Chain E: 6 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	6
β-strand	9-13	5	7
β-strand	18-20	3	8
β-strand	21-24	4	6
β-strand	30-36	7	7
β-strand	42-48	7	7
β-strand	55-56	2	7
β-strand	63-65	3	8
β-strand	72	1	6
β-strand	75-77	3	8
β-strand	86-93	8	7
β-strand	101-102	2	7
β-strand	106-111	6	7
α-helix	114-116	3
β-strand	118	1	9
β-strand	121-125	5	5
α-helix	126-128	3
α-helix	129-135	7
β-strand	137-147	11	5
β-strand	148	1	9
β-strand	152-158	7	10
β-strand	161-163	3	10
β-strand	167-169	3	5
α-helix	173	1
β-strand	174-175	2	5
β-strand	185-194	10	5
α-helix	195-198	4
β-strand	204-211	8	10
β-strand	214	1	11
α-helix	225-226	2
β-strand	228	1	11
β-strand	230-237	8	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
T4H2 T cell receptor alpha chain	D	protein	206	Homo sapiens
T4H2 T cell receptor beta chain	E	protein	241	Homo sapiens
MHC class I antigen, A-2 alpha chain	A	protein	275	Homo sapiens	A0A140T913 (AlphaFold model)
Beta-2-microglobulin	B	protein	100	Homo sapiens	P61769 (AlphaFold model)
Melanocyte protein PMEL	C	protein	9	Homo sapiens	P40967 (AlphaFold model)

Sequence of entity 1 (D), FASTA

>6VMC_1 T4H2 T cell receptor alpha chain (chains D)
MQKEVEQNSGPLSVPEGAIASLNCTYSDRGSQSFFWYRQYSGKSPELIMSIYSNGDKEDG
RFTAQLNKASQYVSLLIRDSQPSDSATYLCAVNALLGNQFYFGTGTSLTVIPNIQNPDPA
VYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNK
SDFACANAFNNSIIPEDTFFPSPESS

Sequence of entity 2 (E), FASTA

>6VMC_2 T4H2 T cell receptor beta chain (chains E)
MGITQSPKYLFRKEGQNVTLSCEQNLNHDAMYWYRQDPGQGLRLIYYSQIVNDFQKGDIA
EGYSVSREKKESFPLTVTSAQKNPTAFYLCASSMGGTYEQYFGPGTRLTVTEDLKNVFPP
EVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPAL
NDSRYALSSRLRVSATFWQDPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRA
D

Sequence of entity 3 (A), FASTA

>6VMC_3 MHC class I antigen, A-2 alpha chain (chains A)
GSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYW
DGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMYGCDVGSDWRFLRGYHQYAYDG
KDYIALKEDLRSWTAADMAAQTTKHKWEAAHVAEQLRAYLEGTCVEWLRRYLENGKETLQ
RTDAPKTHMTHHAVSDHEATLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGT
FQKWAAVVVPSGQEQRYTCHVQHEGLPKPLTLRWE

Sequence of entity 4 (B), FASTA

>6VMC_4 Beta-2-microglobulin (chains B)
MIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKD
WSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Sequence of entity 5 (C), FASTA

>6VMC_5 Melanocyte protein PMEL (chains C)
ILDQVPFSV

Primary citation

Structurally silent peptide anchor modifications allosterically modulate T cell recognition in a receptor-dependent manner. Smith, A.R., Alonso, J.A., Ayres, C.M. et al. Proc Natl Acad Sci U S A (2021) 118:e2018125118. DOI 10.1073/pnas.2018125118

Other PDB entries of the same protein (UniProt A0A140T913 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6O53 1.4 Å, Structure of HLA-A2:01 with peptide MM96
6O4Z 1.5 Å, Structure of HLA-A2:01 with peptide MM92
7QPJ 1.54 Å, Crystal structure of engineered TCR (756) complexed to HLA-A*02:01 presenting MAGE-A10…
6O51 1.55 Å, Structure of HLA-A2:01 with peptide MM90
6O4Y 1.58 Å, Structure of HLA-A2:01 with peptide MM91
7MJ7 1.6 Å, HLA-A*02:01 bound to Neuroblastoma Derived IGFBPL1 peptide
7P3D 1.67 Å, MHC I A02 Allele presenting YLQPRTFLL
7MJ9 1.75 Å, HLA-A*02:01 bound to Neuroblastoma Derived mutant IGFBPL1 peptide
7MJ8 1.79 Å, HLA-A*02:01 bound to Neuroblastoma Derived IGFBPL1 peptide
7SA2 1.85 Å, SARS-CoV-2 spike-derived peptide S1060-1068 (VVFLHVTYV) presented by HLA-A*02:01
2P5E 1.89 Å, Crystal Structures of High Affinity Human T-Cell Receptors Bound to pMHC Reveal Native…
7MKB 1.9 Å, Human leukocyte antigen A*0201 in complex with SARS-CoV-2 epitope YLQPRTFLL

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