6WI9: Human secretin receptor Gs complex
Human secretin receptor Gs complex. Determined by electron microscopy at 4.3 Å resolution. Released 12 Aug 2020.
- Method
- Electron microscopy
- Resolution
- 4.3 Å
- Organisms
- Homo sapiens, Lama glama
- Chains
- 6
- Atoms
- 8,550
- Mol. weight
- 161.08 kDa
- Released
- 12 Aug 2020
Explore 6WI9 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
6WI9 contains 34 α-helices and 51 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 7 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 13-38 | 26 | |
| β-strand | 40-45 | 6 | 1 |
| β-strand | 208-213 | 6 | 1 |
| β-strand | 218-223 | 6 | 1 |
| α-helix | 236-239 | 4 | |
| β-strand | 243-246 | 4 | 1 |
| α-helix | 265-277 | 13 | |
| β-strand | 286-289 | 4 | 1 |
| α-helix | 313-317 | 5 | |
| α-helix | 318-321 | 4 | |
| α-helix | 332-351 | 20 | |
| β-strand | 359-361 | 3 | 1 |
| α-helix | 372-389 | 18 | |
Chain B: 2 helices, 29 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-24 | 21 | |
| α-helix | 30-35 | 6 | |
| β-strand | 47-51 | 5 | 2 |
| β-strand | 58-63 | 6 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 79-83 | 5 | 3 |
| β-strand | 89-93 | 5 | 3 |
| β-strand | 100-105 | 6 | 4 |
| β-strand | 111-116 | 6 | 4 |
| β-strand | 121-125 | 5 | 4 |
| β-strand | 135-139 | 5 | 4 |
| β-strand | 146-151 | 6 | 5 |
| β-strand | 156-161 | 6 | 5 |
| β-strand | 167-170 | 4 | 5 |
| β-strand | 176-179 | 4 | 5 |
| β-strand | 187-192 | 6 | 6 |
| β-strand | 199-203 | 5 | 6 |
| β-strand | 208-211 | 4 | 6 |
| β-strand | 218-222 | 5 | 6 |
| β-strand | 229-234 | 6 | 7 |
| β-strand | 240-245 | 6 | 7 |
| β-strand | 250-254 | 5 | 7 |
| β-strand | 260-264 | 5 | 7 |
| β-strand | 275-278 | 4 | 8 |
| β-strand | 284-288 | 5 | 8 |
| β-strand | 294-298 | 5 | 8 |
| β-strand | 303-308 | 6 | 8 |
| β-strand | 315-317 | 3 | 2 |
| β-strand | 320 | 1 | 2 |
| β-strand | 327-331 | 5 | 2 |
| β-strand | 336-339 | 4 | 2 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 11-13 | 3 | |
| α-helix | 14-17 | 4 | |
| α-helix | 21-24 | 4 | |
| α-helix | 30-43 | 14 | |
Chain N: 2 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 9 |
| β-strand | 10-11 | 2 | 10 |
| β-strand | 17-25 | 9 | 9 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 11 |
| β-strand | 46-51 | 6 | 11 |
| β-strand | 59-60 | 2 | 11 |
| β-strand | 68-73 | 6 | 9 |
| β-strand | 78-84 | 7 | 9 |
| α-helix | 88-90 | 3 | |
| β-strand | 93-97 | 5 | 11 |
| β-strand | 118 | 1 | 11 |
| β-strand | 122-123 | 2 | 11 |
| β-strand | 124-125 | 2 | 10 |
Chain P: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-26 | 25 | |
Chain R: 18 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 31-45 | 15 | |
| α-helix | 46-49 | 4 | |
| β-strand | 69-70 | 2 | 12 |
| β-strand | 75-76 | 2 | 12 |
| β-strand | 85-87 | 3 | 13 |
| α-helix | 94-96 | 3 | |
| β-strand | 103-105 | 3 | 13 |
| α-helix | 132-164 | 33 | |
| α-helix | 173-199 | 27 | |
| α-helix | 213-245 | 33 | |
| α-helix | 254-264 | 11 | |
| α-helix | 266-279 | 14 | |
| α-helix | 296-324 | 29 | |
| α-helix | 337-351 | 15 | |
| α-helix | 353-358 | 6 | |
| α-helix | 363-365 | 3 | |
| α-helix | 367-369 | 3 | |
| α-helix | 370 | 1 | |
| α-helix | 371-375 | 5 | |
| α-helix | 377-379 | 3 | |
| α-helix | 380-389 | 10 | |
| α-helix | 394-407 | 14 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | A | protein | 394 | Homo sapiens | P63092 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 340 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| Nanobody35 | N | protein | 138 | Lama glama | |
| Secretin | P | protein | 27 | Homo sapiens | P09683 (AlphaFold model) |
| Secretin receptor | R | protein | 453 | Homo sapiens | P47872 |
Sequence of entity 1 (A), FASTA
>6WI9_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
Sequence of entity 2 (B), FASTA
>6WI9_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (G), FASTA
>6WI9_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 4 (N), FASTA
>6WI9_4 Nanobody35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA
Sequence of entity 5 (P), FASTA
>6WI9_5 Secretin (chains P)
HSDGTFTSELSRLREGARLQRLLQGLV
Sequence of entity 6 (R), FASTA
>6WI9_6 Secretin receptor (chains R)
DYKDDDDLEVLFQGPAHSTGALPRLCDVLQVLWEEQDQCLQELSREQTGDLGTEQPVPGC
EGMWDNISCWPSSVPGRMVEVECPRFLRMLTSRNGSLFRNCTQDGWSETFPRPNLACGVN
VNDSSNEKRHSYLLKLKVMYTVGYSSSLVMLLVALGILCAFRRLHCTRNYIHMHLFVSFI
LRALSNFIKDAVLFSSDDVTYCDAHRAGCKLVMVLFQYCIMANYSWLLVEGLYLHTLLAI
SFFSERKYLQGFVAFGWGSPAIFVALWAIARHFLEDVGCWDINANASIWWIIRGPVILSI
LINFILFINILRILMRKLRTQETRGNEVSHYKRLARSTLLLIPLFGIHYIVFAFSPEDAM
EIQLFFELALGSFQGLVVAVLYCFLNGEVQLEVQKKWQQWHLREFPLHPVASFSNSTKAS
HLEQSQGTCRTSIIPAGLEVLFQGPHHHHHHHH
Primary citation
Structure and dynamics of the active Gs-coupled human secretin receptor. Dong, M., Deganutti, G., Piper, S.J. et al. Nat Commun (2020) 11:4137-4137. DOI 10.1038/s41467-020-17791-4 · PubMed
Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
- 6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
- 8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
- 7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
- 8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
- 9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
- 6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
- 7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)
Browse structure collections
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