6WNA: Next generation monomeric IgG4 Fc

Next generation monomeric IgG4 Fc. Determined by X-ray diffraction at 2.4 Å resolution. Released 15 Sept 2021.

Method: X-ray diffraction
Resolution: 2.4 Å
Organism: Homo sapiens
Chains: 3
Atoms: 4,728
Mol. weight: 66.6 kDa
Released: 15 Sept 2021

Explore 6WNA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6WNA contains 21 α-helices and 50 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	6-14	9	1
β-strand	24-30	7	1
β-strand	33-39	7	1
β-strand	46-47	2	1
α-helix	49-53	5
α-helix	60-82	23
β-strand	89-99	11	1
β-strand	103-112	10	1
β-strand	115-121	7	1
β-strand	126-128	3	1
α-helix	132-143	12
α-helix	147-153	7
α-helix	154-158	5
α-helix	159-169	11
α-helix	171-175	5
β-strand	178	1	2
α-helix	179-180	2
β-strand	181-188	8	3
β-strand	193-203	11	3
β-strand	204	1	2
β-strand	208-214	7	4
β-strand	217-218	2	4
β-strand	223-228	6	3
β-strand	234-243	10	3
α-helix	247-249	3
β-strand	250-256	7	4
β-strand	263-266	4	4

Chain B: 1 helix, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	5
α-helix	4-5	2
β-strand	6-11	6	6
β-strand	21-30	10	6
β-strand	31	1	5
β-strand	36-41	6	7
β-strand	44-45	2	7
β-strand	50-51	2	6
β-strand	55-56	2	6
β-strand	62-70	9	6
β-strand	78-83	6	7
β-strand	91-94	4	7

Chain H: 11 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	239-243	5	8
α-helix	244-246	3
α-helix	247-251	5
β-strand	258-264	7	8
β-strand	274-279	6	9
β-strand	282-284	3	9
β-strand	288-289	2	8
α-helix	290-292	3
β-strand	293-294	2	10
β-strand	300-301	2	10
β-strand	302-307	6	8
α-helix	310-314	5
β-strand	319-324	6	9
α-helix	331	1
β-strand	332-336	5	9
α-helix	338-340	3
β-strand	344	1	11
α-helix	345-346	2
β-strand	347-351	5	12
α-helix	352-354	3
β-strand	362-372	11	12
β-strand	373	1	11
β-strand	378-383	6	13
β-strand	386-388	3	13
β-strand	391-393	3	12
α-helix	394-396	3
β-strand	397-398	2	12
β-strand	404-413	10	12
α-helix	414-418	5
β-strand	423-428	6	13
α-helix	433-435	3
β-strand	436-441	6	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
IgG receptor FcRn large subunit p51	A	protein	264	Homo sapiens	P55899 (AlphaFold model)
Beta-2-microglobulin	B	protein	99	Homo sapiens	P61769 (AlphaFold model)
Immunoglobulin heavy constant gamma 4	H	protein	207	Homo sapiens	P01861 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6WNA_1 IgG receptor FcRn large subunit p51 (chains A)
HLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGAWVWENQVSWYWEK
ETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGPDNTSVPTAKFALNGEEFMNFDLK
QGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLERGRGNLEWKEPPSMR
LKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVK
SGDEHHYCCIVQHAGLAQPLRVEL

Sequence of entity 2 (B), FASTA

>6WNA_2 Beta-2-microglobulin (chains B)
IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDW
SFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Sequence of entity 3 (H), FASTA

>6WNA_3 Immunoglobulin heavy constant gamma 4 (chains H)
PSVFLFPPKPKDTLYITREPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFN
STYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTFPPEQEE
MTKNQVSLRCLVKGFYPSDIAVEWESNGQPENNYKTTKPVLDSDGSFRLESRLTVDKSRW
QEGNVFSCSVMHEALHNHYTQKSLSLS

Primary citation

In vivo pharmacokinetic enhancement of monomeric Fc and monovalent bispecific designs through structural guidance. Shan, L., Dyk, N.V., Haskins, N. et al. Commun Biol (2021) 4:1048-1048. DOI 10.1038/s42003-021-02565-5 · PubMed

Other PDB entries of the same protein (UniProt P55899 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6C98 1.85 Å, Crystal structure of FcRn bound to UCB-84
6QIO 1.95 Å, Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the…
6C97 2.0 Å, Crystal structure of FcRn at pH3
6C99 2.0 Å, Crystal structure of FcRn bound to UCB-303
9TF0 2.26 Å, Structure of echovirus 18 in complex with neonatal Fc receptor
6NHA 2.38 Å, Crystal structure of SYNT001, a human FcRn blocking monoclonal antibody
6LA6 2.39 Å, Cryo-EM structure of echovirus 11 complexed with its uncoating receptor FcRn at pH 7.4
4K71 2.4 Å, Crystal structure of a high affinity Human Serum Albumin variant bound to the Neonatal…
9MI6 2.41 Å, Crystal structure of human FcRn in complex with nipocalimab Fab fragment
6QIP 2.45 Å, Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the…
6WOL 2.49 Å, Next generation monomeric IgG4 Fc bound to neonatal Fc receptor
5WHK 2.5 Å, Crystal structure of Fab fragment of antibody DX-2507 bound to FcRn-B2M

Browse structure collections

About this viewer

MolViewer shows 6WNA directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.