4K71: High affinity Human Serum Albumin variant

Crystal structure of a high affinity Human Serum Albumin variant bound to the Neonatal Fc Receptor. Determined by X-ray diffraction at 2.4 Å resolution. Released 23 Oct 2013.

Method: X-ray diffraction
Resolution: 2.4 Å
Organism: Homo sapiens
Chains: 6
Atoms: 15,259
Mol. weight: 219.08 kDa
Released: 23 Oct 2013

Explore 4K71 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4K71 contains 99 α-helices and 60 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 38 helices, 0 β-strands

Element	Residues	Length
α-helix	6-14	9
α-helix	16-30	15
α-helix	36-55	20
α-helix	66-74	9
α-helix	81-84	4
α-helix	87-91	5
α-helix	97-104	8
α-helix	120-129	10
α-helix	131-145	15
α-helix	151-168	18
α-helix	174-206	33
α-helix	208-222	15
α-helix	228-246	19
α-helix	250-266	17
α-helix	268-270	3
α-helix	278-280	3
α-helix	283-292	10
α-helix	293-299	7
α-helix	302-304	3
α-helix	305-306	2
α-helix	307-311	5
α-helix	315-321	7
α-helix	323-337	15
α-helix	343-360	18
α-helix	366-370	5
α-helix	373-376	4
α-helix	377-414	38
α-helix	420-437	18
α-helix	442-466	25
α-helix	471-478	8
α-helix	484-490	7
α-helix	498-502	5
α-helix	504-506	3
α-helix	511-513	3
α-helix	518-535	18
α-helix	541-560	20
α-helix	564-570	7
α-helix	572-582	11

Chain B: 10 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	6-14	9	1
α-helix	17-18	2
β-strand	24-30	7	1
β-strand	33-39	7	1
β-strand	46-47	2	1
α-helix	49-53	5
α-helix	60-82	23
β-strand	89-98	10	1
β-strand	104-112	9	1
β-strand	115-121	7	1
β-strand	126-128	3	1
α-helix	132-143	12
α-helix	147-153	7
α-helix	154-158	5
α-helix	159-169	11
α-helix	171-174	4
β-strand	178	1	2
α-helix	179-180	2
β-strand	181-190	10	3
β-strand	193-203	11	3
β-strand	204	1	2
β-strand	208-214	7	4
β-strand	217-220	4	4
β-strand	223-228	6	3
β-strand	234-243	10	3
α-helix	247-249	3
β-strand	250-256	7	4
β-strand	263-265	3	4
β-strand	267	1	3

Chain C: 1 helix, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	5
α-helix	4-5	2
β-strand	6-11	6	6
β-strand	21-30	10	6
β-strand	31	1	5
β-strand	36-41	6	7
β-strand	44-45	2	7
β-strand	50-51	2	6
β-strand	55-56	2	6
β-strand	62-70	9	6
β-strand	78-83	6	7
β-strand	91-94	4	7

Chain D: 38 helices, 0 β-strands

Element	Residues	Length
α-helix	6-14	9
α-helix	16-30	15
α-helix	36-55	20
α-helix	66-74	9
α-helix	81-84	4
α-helix	87-91	5
α-helix	94	1
α-helix	97-104	8
α-helix	120-129	10
α-helix	131-145	15
α-helix	151-168	18
α-helix	174-201	28
α-helix	202-206	5
α-helix	208-222	15
α-helix	228-246	19
α-helix	250-266	17
α-helix	268-271	4
α-helix	278-280	3
α-helix	283-292	10
α-helix	293-299	7
α-helix	306	1
α-helix	307-311	5
α-helix	315-321	7
α-helix	323-337	15
α-helix	343-361	19
α-helix	366-369	4
α-helix	374-376	3
α-helix	377-414	38
α-helix	420-438	19
α-helix	442-466	25
α-helix	471-478	8
α-helix	484-490	7
α-helix	499-502	4
α-helix	511-513	3
α-helix	518-535	18
α-helix	541-558	18
α-helix	567-570	4
α-helix	572-582	11

Chain E: 10 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	6-14	9	8
α-helix	17-18	2
β-strand	24-30	7	8
β-strand	33-39	7	8
β-strand	46-47	2	8
α-helix	49-53	5
α-helix	60-82	23
β-strand	89-98	10	8
β-strand	104-112	9	8
β-strand	115-121	7	8
β-strand	126-128	3	8
α-helix	132-143	12
α-helix	147-153	7
α-helix	154-158	5
α-helix	159-169	11
α-helix	171-174	4
β-strand	178	1	9
α-helix	179-180	2
β-strand	181-190	10	10
β-strand	193-203	11	10
β-strand	204	1	9
β-strand	209-214	6	11
β-strand	217-220	4	11
β-strand	223-228	6	10
β-strand	234-243	10	10
α-helix	247-249	3
β-strand	250-255	6	11
β-strand	263-265	3	11
β-strand	267	1	10

Chain F: 2 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	12
α-helix	4-5	2
β-strand	6-11	6	13
β-strand	21-30	10	13
β-strand	31	1	12
β-strand	36-41	6	14
β-strand	44-45	2	14
α-helix	46	1
β-strand	50-51	2	13
β-strand	55-56	2	13
β-strand	62-70	9	13
β-strand	78-83	6	14
β-strand	91-94	4	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Serum albumin	A, D	protein	585	Homo sapiens	P02768 (AlphaFold model)
IgG receptor FcRn large subunit p51	B, E	protein	274	Homo sapiens	P55899 (AlphaFold model)
Beta-2-microglobulin	C, F	protein	99	Homo sapiens	P61769 (AlphaFold model)

Sequence of entity 1 (A, D), FASTA

>4K71_1 Serum albumin (chains A, D)
DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAE
NCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEV
DVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLP
KLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTK
VHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPA
DLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKC
CAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQMSA
PTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTES
LVNRRPCFSALEVDETYVPKEFNAGTFTFHADICTLSEKERQIKKQTALVELVKHKPKAT
KEQLKAAMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

Sequence of entity 2 (B, E), FASTA

>4K71_2 IgG receptor FcRn large subunit p51 (chains B, E)
AESHLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGAWVWENQVSWY
WEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGPDNTSVPTAKFALNGEEFMNF
DLKQGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLERGRGNLEWKEPP
SMRLKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSL
TVKSGDEHHYCCIVQHAGLAQPLRVELESPAKSS

Sequence of entity 3 (C, F), FASTA

>4K71_3 Beta-2-microglobulin (chains C, F)
IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDW
SFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Primary citation

Crystal structure of an HSA/FcRn complex reveals recycling by competitive mimicry of HSA ligands at a pH-dependent hydrophobic interface. Schmidt, M.M., Townson, S.A., Andreucci, A.J. et al. Structure (2013) 21:1966-1978. DOI 10.1016/j.str.2013.08.022 · PubMed

Other PDB entries of the same protein (UniProt P02768 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9IK6 1.69 Å, Human Serum Albumin crystallized with T4, pH 6.5
6YG9 1.89 Å, Crystal structure of human serum albumin (HSA) in complex with gn-07.
1N5U 1.9 Å, X-ray study of human serum albumin complexed with heme
8RCO 1.9 Å, Structure of Human Serum Albumin in complex with Aristolochic Acid II at 1.9 A resolution
8RCP 1.9 Å, Structure of Human Serum Albumin in complex with Myristic Acid
8RGK 1.9 Å, Structure of Human Serum Albumin in complex with Aristolochic Acid at 1.9 A resolution
8RGL 1.9 Å, Structure of Human Serum Albumin in complex with Aristolochic Acid I at 1.9 A resolution…
9EOD 1.9 Å, Human serum albumin with adamantene carboxylic acid
9CSG 1.91 Å, Human Serum Albumin Bound to Cerastecin Compound 5e
6QIO 1.95 Å, Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the…
9IK7 1.97 Å, Human Serum Albumin crystallized with T4, pH 7.5
7VR0 1.98 Å, Human Serum Albumin

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